Firoj A. TAMBOLI, Vinod D. RANGARI, Suresh G. KILLEDAR, Sagar U. JADHAV, Trupti S. GHATAGE, Vishwajeet P. KORE

Comparative phytochemical evaluation of natural and micropropagated plants of Bacopa monnieri (L.)

Bacopa monnieri Linn. an important medicinal plant of Scrophulariaceae family and is distributed in the wet and marshy lands throughout India, Nepal, Sri Lanka, China, Taiwan, and Hawaii. It is endangered species so it is necessary to preserve germplasm. The objective of this research work is the comparative phytochemical evaluation of natural and micro-propagated plants of Bacopa monnieri (BM). It shows the presence of various phytoconstituents namely, alkaloids, saponins, phenolics, and flavonoids. These phytoconstituents are responsible for anti-oxidant and various other activity of the plant. The protocols for tissue culture has been developed for regeneration of the BM plant from small part (nodal segment). The regenerated plant having the similar potential in terms of phytoconstituents. The protocols of Murashige and Skoog medium can be used for the clonal regeneration of plant on the large scale through which it can be protected and preserved for the long duration in small space. From the comparative phytochemical investigation by HPTLC, the phytoconstituents which occur in the micro propagated plant are similar to that of the natural plant of B. monnieri.

PDF

___

[1] Dey KK. An Introduction to Plant Tissue Culture, New central book agency (P) Ltd., Calcutta, India 1997, pp. 35-44.

[2] Narayanswamy S. Plant Cell and Tissue Culture, Tata McGrawHill Education, New Delhi, India 1994, pp. 22-51.

[3] Chatterji N, Rastogi RP, Dhar ML. Chemical examination of Bacopa monnieria Wettst: Part 1 – Isolation of chemical constituents. Indian J Chem. 1963; 1: 212-215.

[4] Asolkar LV, Kakkar KK, Chakre OS. Second Supplement to Glossary of Indian Medicinal Plants with active Principles (Part I), CSIR, New Delhi, India 1992, pp. 112-113.

[5] Nadkarni KM. The Indian Materia Medica I, Popular Prakashan, Mumbai, India 1982, pp. 624-625.

[6] Sala AV. Indian Medicinal Plants, Orient Longman, Kottakkal, India 1994, pp. 235-236.

[7] Mishra SK, Tiwari KN, Shivna PL, Mishra AK. Micropropagation and Comparative Phytochemical, Antioxidant Study of Bacopa monnieri (L.) Pennell. Res J Pharm Biol Chem Sci. 2015; 6(6): 902-912.

[8] Ambasta SP. Wealth of India Raw Materials, Vol. 2:B. Council of scientific and Industrial Research, New Delhi, India 1988, pp. 23-24.

[9] Jain SK, DeFilipps RA. Medicinal Plants of India, Vol. 2. Reference Publication Inc., Michigan, USA 1991, pp. 556-557.

[10] Soundararajan T, Karrunakaran CM. Micropropagation of Bacopa monnieri through protoplast. Asian J Biotechnol. 2011; 3(2): 135-152.

[11] Heble MR. Production of secondary metabolites through tissue culture and its prospects for commercial use. In: Islam AS. editor. Plant Tissue Culture, Oxford and IBH Publishing Company Pvt. Ltd., New Delhi. 1997, pp. 161-168

[12] George J, Ravishankar GA. Harnessing high value metabolites from plant cells. Ind J Pharm Educ. 1996; 30: 120-130.

[13] Fowler HW. Extraction. In: Carter SJ. editor. Cooper and Gunn’s Tutorial Pharmacy, 6th ed. CBS Publishers and Distributors, New Delhi. 1986, pp. 251-261.

[14] Yelne MB, Borkar GB, Sharma PC. In vitro propagation of Brahmi – Bacopa monnieri (L.) Pennell. Bull Medico – Ethno – Bot Res 1997; 18(3 – 4): 45-50.

[15] Stahl E. Thin Layer Chromatography, second ed., SpringerVerlag Berlin Heidelberg, Germany 1969, pp. 904-905.

[16] Srivastava VK, Srivastava KK. Introduction to Chromatography, S. Chand and Company Ltd., Delhi, India 1991, pp. 8-32.

[17] Sethi PD. High-performance Thin Layer Chromatography, CBS Publisher and distributors, New Delhi, India 1996, pp. 4-28.

[18] Narayana DBA, Agarwal S, Luthra SK, Srinivas NS. A HPTLC method for the quantitative analysis of vasicine in Adhatoda vasica. Indian Drugs. 1995; 32: 583-586.

[19] Chauhan SK, Singh BP, Agrawal S. Determination of Embelin from Embelia ribes by high performance thin layer chromatography. Indian Drugs. 1999; 36: 41-43.