Elif KERVANCIOGLU DEMIRCI, MEHMET ERTAN KERVANCIOĞLU, Gulnaz Nural BEKIROGLU, ŞULE ÇETİNEL

Uzun süreli inkübasyonun ve sıcaklığın oosit aktivatörü fosfolipaz C-zeta aktivitesine etkisi

Amaç: Kapasite olmuş spermin fertilizasyon kapasitesininzamanla üstsel olarak düştüğü bilinmesine rağmen bunun nedenihala araştırılmaktadır. Çalışmada amaç; uzamış inkübasyonunve sıcaklığın sperm fertilizasyon kapasitesi üzerinde etkisinihareketlilik ve oosit aktivasyon faktörü olarak kabul edilenfosfolipaz C-zeta (PLCZ) ile değerlendirmektir.Gereç ve Yöntem: On altı infertil erkek hastadan, ciddioligoasthenospermisi olmayan 11’inin semenlerine dansitegradyan yıkaması uygulandı. Ardından örnekler bölünerek 3 günoda sıcaklığında veya 37°C’de kültüre edildi. Örnekler hareketlilik,PLCZ boyanma yüzdesi ve yoğunluğu açısından günlük olarakdeğerlendirildi.Bulgular: Hem oda sıcaklığı, hem de vücut sıcaklığında herüç parametrede de zamanla azalma gözlendi. Özellikle günler arasıhareketlilikteki değişim ile PLCZ boyanan sperm yüzdesindekideğişim arasında güçlü bir korelasyon bulundu, ancak bukorelasyon zamanla azalmakta idi.Sonuç: İnkübasyon süresinin uzamasıyla, PLCZ boyanmaparametreleri ve hareketlilik arasında korelasyon görülmektedir.PLCZ boyanmasının semen analizi ile rutinde kullanımı, özellikleaçıklanamayan infertilite ve fertilizasyon başarısızlığı vakalarınınspermlerinin fertilizasyon kapasitesinin belirlenmesinde yararsağlayabilecek ve yardımcı üreme teknileri (YÜT) sikluslarındakibaşarıyı artırabilecektir.

Anahtar Kelimeler:

Sıcaklık, Fosfolipaz C-zeta, Fertilizasyon kapasitesi, Sperm, Oosit aktivasyonu, Uzun süreli inkübasyon

The effect of prolonged incubation and temperature on oocyte activator phospholipase C-zeta activity of sperm

Objective: Fertilization capacity of capacitated sperm decreasesexponentially over time, but the reason is still under investigation.The aim of the study was to analyze the effect of prolongedincubation and temperature on sperm fertilization capacity withmotility and staining parameters of phospholipase C-zeta (PLCZ),which is considered to be the oocyte activation factor.Materials and Methods: Density gradient washingwas applied to semen of 11 infertile patients without severeoligoasthenospermia out of 16 patients. The samples were dividedand cultured either at room temperature or at 37°C for 3 days. Thespermatozoons were evaluated for motility, PLCZ staining andintensity daily.Results: All parameters decreased both at room and bodytemperature with increased incubation time. There was a strongcorrelation between the change in motility and in the percentageof PLCZ stained sperms, but this correlation decreased withincubation time.Conclusion: Prolonged incubation results show the correlationbetween PLCZ staining parameters and motility. Routine use ofPLCZ staining together with semen analysis, will be useful topredict fertilization capacity of the sperm especially for unexplainedinfertility and fertilization failure cases, and also can increase thesuccess of assisted reproductive technologies (ART) cycles.

Keywords:

Temperature, Phospholipase C zeta, Fertilization capacity, Sperm, Oocyte activaton, Prolonged incubation,

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