Demir KİRAN, OLGU ENİS TOK, AHMET ÖZER ŞEHİRLİ, Ali Murat GÖKÇE, FERİHA ERCAN

Prenatal sıçan mesanesi üzerine elektromanyetik dalganın etkilerinin morfolojik ve biyokimyasal olarak incelenmesi

Amaçlar: Bu çalışmanın amacı, dijital mobil telefonlarından gelenve en yüksek spesifik absorbsiyon oranı (Specific AbsorbtionRate-SAR) değeri 1,79W/kg olan dijital selüler sistem (DSS)1800-MHz radyasyonunun erkek sıçan mesanesi üzerine etkileriniaraştırmaktır.Gereç ve Yöntem: Sıçanlara mobil telefondan stand-by(Stand-by Fetal grup) ya da kesintisiz iletim modunda yayılanelektromagnetik dalga (EMD Fetal grup), günde 2 saat, embriyonik14. günden doğuma kadar ya da doğumdan sonraki 60. güne kadar(Stand-by ve EMD grupları) uygulanmıştır. Deney gruplarındakitüm hayvanların mesaneleri postnatal 60. günde eter anestezisialtında alınmış ve ışık ve elektron mikroskopik değerlendirmeleriçin hazırlanmıştır. Ürotelyumun bariyer fonkisyonu zonulaokludens 1 ve E-kaderin immunhistokimyası ve transmisyonelektron mikroskobik inceleme için rutenyum kırmızısı boyamasıile değerlendirilmiştir. Oksidatif hasar biyokimyasal teknikler iledeğerlendirilmiştir.Bulgular: Elektromagnetik dalga grubunda, ürotelyumdökülmeleri, mast hücre sayısında artış, sıkı bağlantılarda hasarve interselüler alanda genişlemeler gözlenmiştir. Son olarak,kontrol grubu ile kıyaslandığında, tüm gruplarda malondialdehitseviyesinde yükselme ve glutatyon seviyesinde düşüş gözlenmiştir.Sonuç: Elektromagnetik dalga, maruz kalınan yoğunluk vesüreye paralel olarak, gelişim sürecindeki mesane üzerine olumsuzetki yapmaktadır. Bu değişiklikler mesanede enflamatuvarhastalıklara sebep olabilir.

Anahtar Kelimeler:

Cep telefonu, Elektromanyetik dalga, Mesane, ZO-1, E-kaderin

The morphological and biochemical investigation of prenatal electromagnetic wave effects on urinary bladder in rats

Objectives: The aim of the study was to investigate the effects ofdigital cellular system (DCS) 1800-MHz radiation of a commondigital mobile phone which has the highest specific absorption ratevalue of 1.79 W/kg on the urinary bladders of male rats.Materials and Methods: Rats exposed to electromagneticwave (EMW) emmited by mobile phones on stand-by (Stand-byFetal Group) or discontinious transmission mode (EMW Fetalgroup), 2 hours per day, from embryonic day 14 until parturitionor until postnatal day 60 (Stand-by Fetal Group). The urinarybladders of the animals in experimental groups were removed atpostnatal day 60 and prepared for light and electron microscopicalinvestigation. The barrier function of urothelium was evaluatedusing zonula occludens 1 and E-cadherin immunohistochemistryand ruthenium red staining for transmission electron microscopy.Oxidative damage were evaluated by biochemical techniques.Results: EMW wave group showed desquamation of urothelialcells, increased number of mast cells, degeneration of tight junctions anddilatation of intercellular space. Finally, increase in the malondialdehydeand decrease in the glutathione levels were observed in all experimentalgroups when compared to Those in the control group.Conclusion: Exposure intensity and time length correlate withadverse effects in developing period of the urinary bladder. Thesechanges may lead to urinary bladder inflammatory disorders.

Keywords:

Cellular phone, Electromagnetic wave, Urinary bladder, ZO-1, E-cadherin,

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Kundi M. Mobile phone use and cancer. Occup Environ Med 2004; 61:560-70. doi: 10.1136/oem.2003.007724
Czyz J, Guan K, Zeng Q, et al. High frequency electromagnetic fields (GSM signals) affect gene expression levels in tumor suppressor p53-deficient embryonic stem cells. Bioelectromagnetics 2004; 25:296-307. doi: 10.1002/ bem.10199
Deepinder F, Makker K, Agarwal, A. Cell phones and male infertility: dissecting the relationship. Reprod Biomed Online 2007;15:266-70. doi: http://dx.doi.org/10.1016/S1472- 6483(10)60338-0
Blank, M. Do electromagnetic fields interact with electrons in the Na, K-ATPase? Bioelectromagnetics 2005;26:677-83. doi: 10.1002/bem.20167
Blank M, Soo L. Optimal frequencies for magnetic acceleration of cytochrome oxidase and Na, K-ATPase reactions. Bioelectrochemistry 2001;53:171-4. doi: 10.1016/ S0302-4598(00)00128-8
Aitken RJ, Bennetts LE, Sawyer D, et al. Impact of radio frequency electromagnetic radiation on DNA integrity in the male germline. Int J Androl 2005; 28:171-9. doi: 10.1111/j.1365-2605.2005.00531.x
Yakymenko I, Tsybulin O, Sidorik E, et al. Oxidative mechanisms of biological activity of low-intensity radiofrequency radiation. Electromagn Biol Med 2015; 7:1- 17. doi: 10.3109/15368378.2015.1043557
Ahlbom A, Day N, Feychting M, et al. A pooled analysis of magnetic fields and childhood leukaemia. Br J Cancer 2000; 83:692-8. doi:10.1054/bjoc.2000.1376
Hardell L, Carlberg M, Soderqvist F,et al. Long-term use of cellular phones and brain tumours: increased risk associated with use for > or =10 years. Occup Environ Med 2007; 64:626-32. doi: 10.1136/oem.2006.029751
IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Non-ionizing radition, Part 2: Radiofrequency electromagnetic fields. IARC Monogr Eval Carcinog Risks Hum 2013; 102(Pt 2):1-460.
Oktem F, Ozguner F, Mollaoglu H, et al. Oxidative damage in the kidney induced by 900-MHz-emitted mobile phone: protection by melatonin. Arch Med Res 2005; 36:350-5. doi: 10.1016/j.arcmed.2005.03.021
Koca O, Gokce AM, Akyuz M, et al. A new problem in inflammatory bladder diseases: use of mobile phones! Int Braz J Urol 2014;. 40:520-25. doi: 10.1590/S1677-5538. IBJU.2014.04.11.
Gillenwater, JY, Wein AJ. Summary of the National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases Workshop on interstitial cystitis, National Institutes of Health. J Urol 1988;.140:203-6.
Lewis SA. Everything you wanted to know about the bladder epithelium but were afraid to ask. Am J Physiol Renal Physiol 2000; 278:867-74.
Hauser PJ, Dozmorov MG, Bane BL, et al. Abnormal expression of differentiation related proteins and proteoglycan core proteins in the urothelium of patients with interstitial cystitis. J Urol 2008; 179:764-9. doi:10.1016/j. juro.2007.09.022.
Iavazzo C, Athanasiou S, Pitsouni E, et al. Hyaluronic acid: an effective alternative treatment of interstitial cystitis, recurrent urinary tract infections, and hemorrhagic cystitis? Eur Urol 2007; 51:1534-40. doi:10.1016/j.eururo.2007.03.020.
Brooks AC, Whelan CJ, Purcell WM. Reactive oxygen species generation and histamine release by activated mast cells: modulation by nitric oxide synthase inhibition. Br J Pharmacol 1999; 128:585-90. doi:10.1038/sj.bjp.0702838.
Sant GR, Kempuraj D, Marchand JE, et al. The mast cell in interstitial cystitis: role in pathophysiology and pathogenesis. Urology 2007; 69 (4 Suppl):34-40. doi:10.1016/j. urology.2006.08.1109.
Koca O, Gökçe AM, Öztürk MI, et al. Effects of intensive cell phone (Philips Genic 900) use on the rat kidney tissue. Urol J 2013;10:886-91. doi:10.1016/S1569-9056(13)60309-7.
Bedir R, Tumkaya L, Şehitoğlu I, et al. The effect of exposure of rats during prenatal period to radiation spreading from mobile phones on renal development. Ren Fail 2015; 37:305- 9. doi:10.3109/0886022X.2014.985995.
Qiu LB, Ding GR, Li KC, et al. The role of protein kinase C in the opening of blood-brain barrier induced by electromagnetic pulse. Toxicology 2010; 273:29-34. doi:10.1016/j.tox.2010.04.013.
Lui WY, Cheng CY. Regulation of cell junction dynamics by cytokines in the testis: a molecular and biochemical perspective. Cytokine Growth Factor Rev 2007; 18:299-311. doi: 10.1016/j.cytogfr.2007.04.009.
Wang XW, Ding GR, Shi CH, et al. Effect of electromagnetic pulse exposure on permeability of blood-testicle barrier in mice. Biomed Environ Sci 2008;21:218-21. doi:10.1016/ S0895-3988(08)60032-X.
Blackman CF, Elder JA, Weil CM, et al. Induction of calciumion efflux from brain tissue by radio-frequency radiation: Effects of modulation frequency and field strength. Radio Science 1979; 14:93-8. doi:10.1029/RS014i06Sp00093.
Shie JH, Kuo HC. Higher levels of cell apoptosis and abnormal E-cadherin expression in the urothelium are associated with inflammation in patients with interstitial cystitis/painful bladder syndrome. BJU Int 2011;108(2 Pt 2):E136-41. doi:10.1111/j.1464-410X.2010.09911.x.
Liu HT, Shie JH, Chen SH, et al. Differences in mast cell infiltration, E-cadherin, and zonula occludens-1 expression between patients with overactive bladder and interstitial cystitis/bladder pain syndrome. Urology 2012;80:225.e13. doi:10.1016/j.urology.2012.01.047.