VİLDAN KARPUZ, Aslı GÖKTÜRK, Meral KOYUTURK

The effects of sperm morphology and motility on the outcomes of intracytoplasmic sperm injection

Amaç: İntrasitoplazmik sperm enjeksiyonu (ICSI) tedavi sonuçları, sperm morfoloji ve motilite sınıflandırmaları ile karşılaştırılarak değerlendirildi; bu çerçevede Kruger kesin kriterlerinin veya motilitenin, bu sonuçları tahminde yardımcı olup olamıyacağı araştırıldı. Yöntem: Kliniğe başvuran infertil çiftlerden, 242 si seçilerek ICSI tedavisi uygulandı. Çalışma grubundaki kadın partnerlerde Metafiz II oosit sayısı en az 5 ve üzerinde, erkek partnerlerde ise yanlızca semen sıvısında sperm bulunması gereği öngörüldü. Semen analizi ve motilite değerlendirmesi Dünya Sağlık Örgütü (WHO) kriterlerine göre gerçekleştirildi; sperm morfolojisi ise “Kruger’in kesin kriterleri”ne göre değerlendirildi. Bulgular: Sperm morfoloji ve motilite yüzdelerine göre oluşturulan gruplar içerisinde ICSI sonuçlarını değerlendirme parametrelerinden, fertilizasyon ve gebelik oranları arasında anlamlı bir fark saptanmadı. Sonuç: Sperm motilite ve morfolojisi in vitro fertilizasyon (IVF) sonuçlarının değerlendirilmesinde en iyi parametreler olarak kabul edilmektedir. Oysaki bizim bulgularımız ICSI sonuçlarının IVF için değerli olan bu parametrelerden bağımsız olduğunu göstermektedir.

Sperm morfolojisi ve motilitesinin intrasitoplazmik sperm enjeksiyon sonuçları üzerine etkileri

Objective: The outcomes of ICSI (Intracytoplasmic Sperm Injection) treatments were evaluated and compared with sperm morphology and motility classifications in order to determine whether strict criteria or motility could aid in predicting the ICSI outcomes. Materials and methods: The infertile couples admitted to the clinic, 242 of them were selected and ICSI treatment was performed. In study group, female partners required having at least 5 oocytes at metaphase II and for male partners only the presence of spermatozoa cells in the semen fluid was necessary. Semen analysis and motility was performed according to WHO (World Health Organisation) criteria and sperm morphology was assessed according to Kruger’s criteria. Results: There was no significant difference for the ICSI outcome assessment parameters indicated that fertilization and pregnancy rates between in the groups based on the percentages of sperms morphology and motility. Conclusion: Sperm morphology and motility were accepted as best parameters to evaluate the outcomes of IVF (In Vitro Fertilization). However, our results showed that ICSI outcomes were independent from these valuable parameters for IVF.

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