Buket CİCİOĞLU ARIDOĞAN, Rabia Can SARINOĞLU

Probiyotiklerin alerji üzerine etkisinin araştırılması

Amaç: Bu çalışmada, insan bağırsak florasından izole edilerek tanımlanan Lactobacillus spp. ve Enterococcus spp. suşlarından hazırladığımız probiyotik karışım ve standart probiyotik suş Lactobacillus GG (LGG)’nin alerjik immün cevaplar üzerine etkilerinin hayvan modelinde araştırılması amaçlanmıştır. Gereç ve Yöntem: Probiyotik nitelikleri tanımlanan üç Enterococcus faecalis, sekiz Lactobacillus plantarum ve iki Lactobacillus rhamnosus suşu, probiyotik karışıma alınmıştır. Çalışma gruplarındaki Balb-c fareler probiyotik karışım ve standart suş LGG, kontrol gruplarındaki fareler ise skim milk ile 28 gün boyunca beslenmiştir. Çalışma grupları ve pozitif kontrol grubundaki fareler 14. ve 21. günlerde ovalbumin (OVA)’nın intraperitoneal enjeksiyonu ile immünize edilmiştir. Negatif kontrol grubuna fosfat tamponlu su enjekte edilmiştir. Fare serumlarında OVA spesifik IgE düzeyleri ELISA yöntemi ile çalışılmıştır.Bulgular: En dikkat çekici sonuçlarımız; hayvan deneyinde OVA ile immünizasyon yapılan pozitif kontrol grubunda negatif kontrol grubu ile karşılaştırıldığında OVA spesifik IgE düzeylerinin anlamlı olarak yüksek bulunması (p

Investigation of the effects of probiotics on allergy

Objectives: To investigate the effects of a probiotic mixture that was isolated from human gut flora, and a standard probiotic strain Lactobacillus GG (LGG) on allergic immune responses in an animal model.Materials and Methods: Three Enterococcus faecalis, 8 Lactobacillus plantarum, and 2 Lactobacillus rhamnosus strains were included in the mixture. Balb-c mice in the study groups were given the probiotic mixture, and standard strain LGG, and animals in the control groups were given skimmed milk for 28 days. The mice in the study groups and the positive control group were immunized with an intraperitoneal injection of ovalbumin (OVA) on days 14 and 21. An enzyme-linked immunosorbent assay was used to study the OVA-specific IgE levels in the mice serums.Results: The most remarkable results were that OVA-specific IgE levels were significantly higher (P

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