Samet TEKİN, Yusuf DAĞ, Merve BOLAT, Emin ŞENGÜL

Ratlarda Bisphenol A ile İndüklenen Nefrotoksisitede Kidney İnjury Molecule -1 Üzerine p-Kumarik Asitin Etkileri

BisfenolA(BPA); polikarbonat plastiklerin, epoksi reçinelerin ve diğer birçok polimer malzemelerin sentezinde yaygın olarak kullanılan endüstriyel bir kimyasaldır. Seri üretimleri ve yaygın kullanım alanları nedeniyle çevrede bol miktarda bulunmaktadır. BPA, birçok doku ve organda hasara yol açmaktadır. Bu çalışmada BPA ile indüklenen nefrotoksisitede kidney injury molecule-1(KİM-1) seviyesi üzerine p-kumarik asit(PCA)’in etkileri araştırıldı. Çalışmada erkek erişkin 40 adet rat kullanıldı. Her grupta 8 ratın bulunduğu 5 deney grubu oluşturuldu. Kontrol grubuna 14 gün boyunca intragastrik(i.g.) 1ml serum fizyolojik verildi. BPA(100) grubuna 14 gün boyunca 100 mg/kg dozunda i.g. BPA verildi. PCA(50)+BPA(100) ve PCA(100)+BPA(100) gruplarına 14 gün boyunca 100mg/kg BPA ile birlikte sırasıyla 50 ve 100 mg/kg dozunda i.g. PCA verildi. PCA(100) grubuna ise 14 gün boyunca 100 mg/kg dozunda PCA verildi. Deneyin 15. günü, ratlar anestezi altına alındı ve sonrasında böbrek dokuları toplandı. Böbrek dokularında KİM-1 düzeyleri analiz edildi. Sonuçlar gruplar arasında değerlendirildi. BPA(100) grubunda KİM-1 düzeylerinin kontrole göre önemli düzeyde arttığı belirlendi. PCA’nın düşük dozunun KİM-1 düzeyindeki artışı anlamlı düzeyde önleyemedi. PCA(100)+BPA(100) ve PCA(100) gruplarında KİM-1 seviyelerinin kontrol grubuna benzediği belirlendi. Bu çalışmanın sonucunda, ratlarda BPA ile indüklenen nefrotoksisitede PCA’nın özellikle yüksek dozunun koruyucu etkili olduğu ve KİM-1 düzeylerindeki artışı önemli düzeyde önlediği belirlendi.

Anahtar Kelimeler:

Bisfenol A, KİM-1, Nefrotoksisite, p-kumarik asit, Rat

Effects of p-Coumaric Acid on Kidney Injury Molecule-1 in Bisphenol A-induced Nephrotoxicity in Rats

Bisphenol A(BPA); It is an industrial chemical widely used in the synthesis of polycarbonate plastics, epoxy resins and many other polymer materials. It is abundant in the environment due to its mass production and widespread use. BPA causes damage to many tissues and organs. In this study, the effects of p-coumaric acid(PCA) on kidney injury molecule-1(KIM-1) level in BPA-induced nephrotoxicity were investigated. Forty male adult rats were used in the study. 5 experimental groups with 8 rats in each group were formed. The control group was given 1 ml of saline intragastric (i.g.) for 14 days. BPA(100) group was given 100 mg/kg i.g. PCA(50)+BPA(100) and PCA(100)+BPA(100) groups were given 100mg/kg BPA together with i.g PCA at a dose of 50 and 100mg/kg, respectively, for 14 days. PCA(100) group was given PCA at a dose of 100 mg/kg for 14 days. On the 15th day of the experiment, the rats were anesthetized and then kidney tissues were collected. KIM-1 levels in kidney tissues were analyzed. Results were evaluated among groups. It was determined that, KIM-1 levels were significantly increased in the BPA(100) group compared to the control group. The low dose of PCA could not significantly prevent the increase in KIM-1 level. It was determined that KIM-1 levels in PCA(100)+BPA(100) and PCA(100) groups were similar to the controlgroup. As a result of this study, it was determined that, especially high dose of PCA, had a protective effect in BPA-induced nephrotoxicity in rats and significantly prevented the increase in KIM-1 levels.

Keywords:

Bisphenol A, KİM-1, Nephrotoxicity, p-Coumaric acid, Rat,

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