Yağmur Nil DEMİREL, Mürüvvet DÜZ, İlkay DOĞAN, Zeki GÜRLER

The Relation between Biochemical Parameters, Milk Amyloid A, Somatic Cell Count, and Some Pathogens in Buffalo Milks

Being resistant to hard environmental conditions and diseases makesBuffalo to have its valuable milk. Microbial contamination may occurdue to undesirable conditions such as mastitis, environmentalcontamination, and stress. If microorganisms are not removed fromthe milk, it causes many production disadvantages includinginadequacy of production, failure of fermentation and shortening ofthe shelf life. This study was conducted to determine the relationshipbetween somatic cells count (SCC), the presence of some pathogens,and milk amyloid A (MAA) in the buffalo milk. In addition, oxidativestress in buffalo milk was evaluated. For this purpose, 70 sampleswere collected and Enterobacteriaceae, coliform microorganisms,Escherichia coli, Salmonella spp. analyses were performed.Biochemical parameters [Malondialdehyde (MDA), glutathione(GSH), nitric oxide (NO), catalase, superoxide dismutase (SOD), andtotal antioxidant capacity (TAC)] and serological parameter (MAA)were measured. The SCC was not statistically different according toEnterobacteriaceae, coliform microorganisms, E. coli groups. WhileMDA, NO, SOD, and TAC values were not statistically differentaccording to the SCC groups, GSH and catalase values werestatistically different. MAA values were statistically significantcompared to the SCC groups. Moreover, there was a positivecorrelation between MAA and MDA/SCC. Detection of MAA mayprevent the mixing of healthy and mastitic milk. Therefore, morereliable buffalo milk products will be presented to consumption.

Manda Sütlerindeki Somatik Hücre Sayısının Bazı Patojenler, Biyokimyasal Parametreler ve Süt Amiloid A ile İlişkisi

Mandaların olumsuz çevre koşullarına, hastalıklara karşı dirençli yapısı sütünü de değerli kılmaktadır. Manda sütlerine mastitis, çevresel bulaşma, stres gibi istenmeyen bazı durumlara bağlı olarak mikrobiyel bulaşma olabilir. Bu mikroorganizmaların sütten uzaklaştırılamaması sonucunda yetersiz üretim, fermantasyonun gerçekleşmemesi, ürünlerin raf ömrünün kısalması gibi üretim sorunlarına neden olmaktadır. Bu nedenle, manda sütlerindeki somatik hücre sayısının bazı patojenlerin varlığı ve süt amiloid A ile ilişkisinin belirlenmesi amaçlanmıştır. Buna ilaveten manda sütlerinde meydana gelen oksidatif stres değerlendirilmiştir. Bu amaç doğrultusunda, toplanan manda sütlerinde Enterobacteriaceae, koliform mikroorganizmalar, Escherichia coli ve Salmonella spp. analizleri yapılmıştır. Biyokimyasal parametrelerden malondialdehit (MDA), glutatyon (GSH), nitrik oksit (NOx), katalaz (KAT), süperoksit dismutaz (SOD), total antioksidan seviye (TAS) ölçülmüştür. Serolojik olarak ise süt amiloid A (SAA) seviyesi belirlenmiştir. Bu analiz sonuçlarına göre Enterobacteriaceae,koliform mikroorganizma, E. coli gruplarına göre SHS ortalamaları istatistiksel olarak anlamlı farklılık göstermemiştir. MDA, NOx, SOD, TAS değerleri SHS gruplarına göre istatistiksel farklılık göstermezken; GSH ve KAT değerlerinin SHS gruplarına göre istatistiksel olarak farklı olduğu tespit edilmiştir. SAA değeri SHS gruplarına göre istatistiksel olarak anlamlı farklılık göstermiştir. Buna ilaveten SAA ile MDA ve SHS değeri arasında istatistiksel olarak anlamlı, düşük düzeyde, pozitif yönde bir korelasyon tespit edilmiştir. Manda sütlerinde süt amiloid A’nın tespiti ile, hastalıklı hayvan sütlerinin sağlıklı sütlerle karışmasına engel olunarak daha güvenilir manda süt ve süt ürünleri tüketime sunulma olanağı kazandırır.

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