Siyah Alaca ve Doğu Anadolu Kırmızısı Irkına Ait Sığırların Mycoplasma bovis ile Enfekte Sütlerinden Köken Alan Eksozomlardaki Yangı Ile Ilişkili miRNA’ların Ekpresyon Profili

Mikoplazma bovis, sığırlarda mastitis, artrit ve pnömoni gibi çeşitli klinik hastalıklarla ilişkili önemli bir ajandır. Bu çalışmada, Türkiye'de yetiştirilen Siyah Alaca (SA) ve Doğu Anadolu Kırmızısı (DAK) sığırlarına ait Mycoplasma bovis ile enfekte subklinik mastitli ve normal sütlerde inflamasyon ile ilişkili miRNA adaylarının belirlenmesi amaçlandı. Mastitli ve normal sığırlardan elde edilen sütteki miRNA'ların ekspresyon seviyeleri qRT-PCR ile analiz edildi. MiR-21, miR-146a, miR-155, miR-222, miR-383, miR-200a, miR-205, miR-122, miR-182'nin ekspresyon düzeylerinin her iki sığıra ait mastitli sütte arttığı gözlendi. Bununla birlikte, miR-21 ve miR222'nin Holştayn sığırının mastitli sütünde önemli ölçüde arttığı, miR-146a ve miR-383'ün ise DAK sığırının mastitli sütünde önemli ölçüde arttığı belirlendi. Sonuç olarak, subklinik mastitli sütte ekspresyon düzeyi artan miRNA adayları Holştayn ve DAK sığırlarında belirlendi. Araştırmadan elde edilen bulgular, subklinik mastitis sütünde ekspresyon düzeyi artan miRNA'ların Mycoplasma bovis'in neden olduğu subklinik masitit tanısında biyobelirteç olarak kullanılabileceğini göstermiştir.

Expression Profiles of Inflammation-related MicroRNAs in Mycoplasma bovis Infected Milk of Holstein-Friesian and Doğu Anadolu Kırmızısı Cows

Mycoplasma bovis is an important pathogen associated with severalclinical diseases in cattle, such as mastitis, arthritis, and pneumonia.TableIn this study, we aimed to identify miRNA candidate biomarkersassociated with inflammation in Mycoplasma bovis -infected milksamples and normal milk samples of Holstein-Friesian (HF) and DoğuAnadolu Kırmızısı (DAK) cows in Turkey. The expression levels ofmiRNAs in milk from mastitis-infected cows and uninfected cows wereanalyzed using a qRT-PCR. The results revealed that miR-21, miR146a, miR-155, miR-222, miR-383, miR-200a, miR-205, miR-122, andmiR-182 were upregulated in mastitis milk. Among the miRNAcandidate biomarkers, miR-21 and miR-222 were significantlyupregulated only in mastitis milk samples from HF cows, and miR146a and miR-383 were significantly upregulated only in mastitismilk samples from DAK cows. These results shed light on miRNAcandidate biomarkers in milk from HF and DAK cows with subclinicalmastitis. The upregulated miRNAs detected in the present study couldbe used as biomarkers in the diagnosis of subclinical mastitis causedby Mycoplasma bovis.

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