Kanola Yağı İçeren Yemlerle Beslenen Avrupa Deniz Levreği (Dicentrarchus labrax L.)’nin Sindirim Kanalı Boşaltım Süresinin Belirlenmesi

Bu çalışmada Avrupa deniz levreği (Dicentrarchus labrax) yemlerindekullanılan, balık yağına ikame olarak, üç farklı oranda (0, 50 ve 100)kanola yağı (Kanola, K) içeren yemlerle (20.4 g) 10 hafta boyuncavücut ağırlıklarının %2’si olacak şekilde beslenmiştir. Beslemedenemesinden sonra, her tanka (500 L) 20 adet levrek (78.6 ± 3.58gram) üç tekerrürlü olarak stoklanmıştır. Sindirim kanalınınboşaltım süresinin belirlenmesi için, örneklemeden önce bireyler 72saat aç bırakılarak sindirim sisteminin tamamen boşaltılmasısağlanmıştır. Örnekleme başlamadan önce tüm balıklar tek öğün “adlibitum” olarak beslenmiştir. Her deneme grubundan 0., 8., 16., 24.,32. ve 48. saatlerde tesadüfi olarak 9 adet balık örneklenmiş vesindirim kanalında mide, ön bağırsak ve son bağırsak olmak üzere üçfarklı bölgede kalan yemler tespit edilmiştir. Mide, ön bağırsak vebağırsaklardaki en kısa boşaltım sürelerinin sırasıyla 32.2; 60.9 ve50.4 saat ile K0 grubunda (%0 kanola yağı) olduğu bulunmuştur, enuzun sürenin ise sırasıyla 41.9; 101.3 ve 70.2 saat ile K100 (%100kanola yağı) grubunda olduğu belirlenmiştir (P

Determination of Gastric Evacuation Time for European Sea Bass (Dicentrarchus labrax L.) with Feeds Containing Canola Oil

In this study, European sea bass (Dicentrarchus labrax) (20.4) was fed 2%BW for 10 weeks with three different diets containing 0, 50 and 100 of canola oil (Canola, K), as replacement to fish oil. For this purpose, 20 sea bass (78.6± 3.58 grams) (500 L) were stocked in each tank in triplicate. For the determination of the GET, the individual was left fasted for 72 hours before sampling to ensure complete digestion of the digestive tract. Apart from these fasting periods, the individuals were fed "ad libitum". Nine fish were randomly sampled at 0., 8., 16., 24., 32. and 48. hours from each experimental group and the digestive tract were examined in three different sections: the stomach, the anterior and the posterior intestine. The shortest gastric evacuation time (GET) in the stomach, anterior and posterior intestines were found as 32.2, 60.9 and 50.4 h in K0 (0%K) group, while longest as 41.9, 101.3 and 70.2 h in 100-group, respectively. Final data showed that the increase of the ratio of canola used as a substitute to fish oil in the feed, GET was linearly extended in the whole digestive system and this will limit the use of this plant oil source in feed formulations at high rates.

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