Elif AYAZOGLU DEMIR, Selim DEMIR, Nihal TURKMEN, Ibrahim TURAN

Rosa pimpinellifolia Ekstraktının İnsan Tümör Hücrelerinin Çoğalmasına Etkisi

Bu çalışmanın amacı, R. pimpinellifolia'nın antioksidan özelliklerini ve insan kolon (WiDr), karaciğer (HepG2) ve akciğer (A549) kanser hücreleri üzerindeki sitotoksik etkisini normal bir fibroblast (BJ) hücre hattına kıyasla değerlendirmektir. Ekstraktın antioksidan özellikleri spektrofotometrik yöntemler kullanılarak belirlenirken, sitotoksik etkisi ise MTT testi kullanılarak değerlendirildi. Ekstraktın toplam fenolik içeriği, toplam flavonoid içeriği ve indirgeme gücü değerleri g örnek başına sırasıyla 16.4±0.4 mg gallik asit eşdeğeri, 5.2±0.2 mg kuersetin eşdeğeri ve 34.3±2.4 mg troloks eşdeğeri olarak hesaplandı. Ekstrakt BJ hücrelerine kıyasla üç kanser hücresi hattı üzerinde doza bağımlı bir şekilde seçici sitotoksik etki sergilemesine rağmen, en yüksek seçicilik indeks değeri (2.7 kat) WiDr hücre hattı için elde edildi. Sonuç olarak, bu çalışma R. pimpinellifolia'nın kanser hücreleri üzerindeki sitotoksik etkisini gösteren ilk çalışmadır. Bu sitotoksik etkinin moleküler mekanizmasını göstermek ve in vivo çalışmaların yapılması gelecekteki klinik çalışmalar için yol gösterici olabilir.

The Effect of Rosa pimpinellifolia Extract on the Proliferation of Human Tumor Cells

The aim of this study was to evaluate the antioxidant properties of R. pimpinellifolia and its cytotoxic effect against human colon (WiDr), liver (HepG2) and lung (A549) cancer cells compared with a normal fibroblast (BJ) cell line. The antioxidant properties of extract was determined using spectrophotometric methods, while the cytotoxic effect of extract was evaluated using MTT assay. The total phenolic content, total flavonoid content and reducing power values of extract were calculated as 16.4±0.4 mg gallic acid equivalent, 5.2±0.2 mg quercetin equivalent and 34.3±2.4 mg trolox equivalent per g sample, respectively. Although the extract exhibits selective cytotoxic effect on three cancer cell lines in a dose-dependent manner compared to BJ cells, the highest selectivity index value (2.7-fold) was obtained for the WiDr cell line. In conclusion, this is the first study to demonstrate the cytotoxic effect of R. pimpinellifolia on cancer cells. To demonstrate the molecular mechanism of this cytotoxic effect and to carry out in vivo studies may be a guide for future clinical studies.

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