Elif AYAZOĞLU DEMİR, Serap Özer YAMAN, İbrahim TURAN, Yüksel ALİYAZICIOĞLU, Sıla Özlem ŞENER, Rezzan ALİYAZICIOPĞLU, Selim DEMİR

Dorycnium pentaphyllum'un Fitokimyasal Analizi ve Serviks Kanseri Hücreleri Üzerindeki Antiproliferatif Etkisi

Bu çalışmanın amacı, D. pentaphyllum'un fitokimyasal analizini gerçekleştirmek ve bitkinin insan serviks kanseri (HeLa) hücreleri üzerindeki sitotoksik aktivitesini olası mekanizmalarla birlikte araştırmaktır. Ekstraktın toplam fenolik içerik (TPC) ve fitokimyasal analizi, sırasıyla spektrofotometrik yöntem ve RP-HPLC ile değerlendirildi. Ekstraktın sitotoksik etkisi MTT metodu kullanılarak belirlendi. Ekstraktın sitotoksik etkisinin mekanizması ise apoptoz, hücre döngüsü ve mitokondriyal membran potansiyeli (MMP) açısından florometrik yöntemler kullanılarak araştırıldı. Ekstraktın TPC değeri g örnek başına 68.9±2.3 mg gallik asit eşdeğeri olarak hesaplandı ve kuersetin ekstrakt içindeki ana fenolik bileşik olarak belirlendi. Ekstrakt normal fibroblast hücrelerine kıyasla, HeLa hücrelerinde seçici bir sitotoksik etki (6.5 kat) gösterdi. Ekstraktın sitotoksik etkisinin, hücre döngüsünü S fazında durdurmasından ve azalmış MMP nedeniyle artmış apoptozdan kaynaklandığı bulundu. Ekstraktın hücre sinyalizasyonu üzerindeki detaylı etkilerinin belirlenmesi için daha ileri çalışmalar gerekmektedir.

Phytochemical Analysis of Dorycnium pentaphyllum and Its Antiproliferative Effect on Cervix Cancer Cells

The purpose of this study was to investigate the phytochemicalanalysis of D. pentaphyllum and its cytotoxic activity on humancervical cancer (HeLa) cells with possible mechanisms. Total phenoliccontents (TPC) and phytochemical analysis of the extract wereevaluated using spectrophotometric method and RP-HPLC,respectively. The cytotoxic effect of the extract was evaluated usingthe MTT assay. The mechanism of the cytotoxic effect of the extractwas also evaluated in terms of apoptosis, cell cycle, and mitochondrialmembrane potential (MMP) using the fluorometric methods. The TPCof the extract was calculated as 68.9±2.3 mg gallic acid equivalent perg sample and quercetin was determined as the major phenolic in theextract. The extract showed a selective cytotoxic effect (6.5-fold) onHeLa cells compared to normal fibroblast cells. The cytotoxic effect ofthe extract was found to be due to the arresting of the cell cycle in theS phase and increasing apoptosis through decreased MMP. Furtherstudies are needed for the comprehensive effects of the extract on cellsignaling.

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