Fevziye Burcu ŞİRİN, Sevinç İNAN, Gökhan CESUR, Mine GEÇGELEN CESUR, Mehmet DİNÇER BİLGİN, Ergun Cem KÖKEN, Afra ALKAN, Tuna ÖNAL

Düşük Şiddetli Darbeli Ultrasonun Ortodontik Diş Hareketi Üzerine Etkilerinin Histolojik ve Biyokimyasal Olarak İncelenmesi

Amaç: Çalışmamızın amacı düşük şiddetli darbeli ultrasonun ratlarda ortodontik diş hareketi üzerine etkilerini araştırmaktır. Gereç ve Yöntem: Bu çalışmada Adnan Menderes Üniversitesi Hayvan Laboratuvarı'ndan 12 haftalık 40 yetişkin erkek Wistar albino rat kullanılmıştır. Ratlar her bir grupta on tane olmak üzere dört gruba ayrılmıştır. Grup 1, tedavi edilmemiş kontrol grubuydu. Grup 2'de dişleri hareket ettirmek için ortodontik spring kullanılmıştır. Grup 3 ve 4'te, ortodontik tedaviye ek olarak 14 gün boyunca 16 J/cm2 veya 48 J/cm2'de düşük şiddetli darbeli ultrason uygulanmıştır. 14. günde diş hareket miktarı ölçülmüştür. Serum kemik alkalen fosfataz (BALP) ve Ctelopeptid tip I kollajen (CTX-I) düzeyleri biyokimyasal olarak analiz edilmiştir. Histolojik olarak osteoklast, osteoblast ve inflamatuar hücre sayısı, kılcal damar yoğunluğu ve yeni kemik oluşumu belirlenmiştir. Nükleer faktör-kappa B ligand (RANKL), osteoprotegerin (OPG), vasküler endotelyal büyüme faktörü (VEGF) ve transforme edici büyüme faktörü-β (TGF-β) immünohistokimyasal boyama kullanılarak değerlendirilmiştir. Bulgular: Grup 4'teki BALP ve CTX-I düzeyleri kontrol grubuna göre anlamlı olarak daha yüksekti (p

Histological and Biochemical Investigation of the Effects of Low Intensity Pulsed Ultrasound on Orthodontic Tooth Movement

Objective: The goal of our study is to assess the effects of low intensity pulsed ultrasound on orthodontic tooth movement in rats. Methods: For this study, 40 adult male Wistar albino rats (12-weeks old age) were used from the Animal Laboratory at Adnan Menderes University. Rats were divided into four groups each of ten. Group 1 was the untreated as a control. In group 2, an orthodontic spring was used to move teeth. For groups 3 and 4, orthodontic treatment was combined with low intensity pulsed ultrasound at 16 J/cm2 or 48 J/cm2 for 14 days, respectively. Tooth movement was measured at the last day of treatment. Serum bone alkaline phosphatase (BALP) and C-telopeptide type I collagen (CTX-I) levels were analyzed biochemically. The number of osteoclasts, osteoblasts and inflammatory cells, capillary density and new bone formation was determined histologically. Receptor activator of nuclear factorkappa B ligand (RANKL), osteoprotegerin (OPG), vascular endothelial growth factor (VEGF) and transforming growth factor-β (TGF-β) were assessed using immunohistochemical staining. Results: BALP and CTX-I levels in group 4 were significantly higher compared to control (p

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ISSN: 1309-3878
Yayın Aralığı: 3
Başlangıç: 2009
Yayıncı: Düzce Üniversitesi Tıp Fakültesi Aile Hekimliği AD adına Yrd.Doç.Dr.Cemil Işık Sönmez