Mücahit GÜNAYDIN, Süha TÜRKMEN, Yunus KARACA, Özgür TATLI, Furkan YILDIRIM, Buket ALTİNKAYNAK AKCAN, Abdülkadir GÜNDÜZ

The Diagnostic Value of Protein Carbonyl Levels in Acute Carbon Monoxide Intoxication

Objective: Carbon monoxide (CO) is the main cause of intoxication-related mortality and morbidity in developed countries. It is responsible for more than half of fatal intoxications in many countries. The purpose of this study was to determine the diagnostic value of protein carbonyl (PC), a good marker of oxidative stress, in association with oxidative stress resulting from hypoxia emerging in patients with acute CO intoxication. Methods: Thirty-four patients diagnosed with acute CO intoxication at the Emergency Department and 38 healthy volunteers were included in the study. Patients’ PC levels at time of admission and after treatment were compared with those of a control group. Results: No statistically significant difference was observed among PC levels at time of admission in the patient and control groups (p =0.305, patient group 0.025 ± 0.01, control group 0.026 ± 0.01). A significant decrease was determined in post-treatment PC levels in the patient group compared to those at time of admission (p = 0.006, admission 0.025 ± 0.01, post-treatment 0.017 ± 0.008). No significant correlation was determined between patients’ carboxyhemoglobin (CO-Hb) levels and PC levels at time of admission (Correlation coefficient = -0.006, p= 0.971). Conclusion: We think that PC is not suitable for use as a biomarker in the acute period in patients with CO intoxication.

Keywords:

Carbon monoxide poisoning, Oxidative stress, Protein carbonyl,

PDF

___

1. Hampson NB, Scott KL, Zmaeff JL. Carboxyhemoglobin measurement by hospitals: implications for the diagnosis of carbonmonoxide poisoning. J Emerg Med 2006;31:13-6
2. Dubrey SW, Chebab O, Ghonim S. Carbon monoxide poisoning: an ancient and frequent cause of accidental death. Br J Hosp Med (lond). 2015;76(3):159-62
3. Chiew AL, Buckley NA. Carbon monoxide poisoning in the 21st century. Critical Care 2014;18:221
4. Hampson NB, Hauff NM. Carboxyhemoglobin levels in carbon monoxide poisoning: do they correlate with the clinical picture? Am J Emerg Med 2008;26:665-9
5. Ozcan O, Erdal H, Cakırca G, et al. Oxidative stres and its impact on intracellular lipids, protein and DNA. J Clin İnvest 2015;6(3):331-6
6. Schacter E. Protein oxidative damage. Methods Enzymol. 2000;319:428-36
7. Kayalı R, Cakatay U. Protein oksidasyonunun ana mekanizmaları. Cerrahpasa J Med 2004;35:83-9
8. Dalle-Done I, Giustarini D, Colombo R, et al. Protein carbonylation in human diseases. Trends Mol Med. 2003; 9(4):169-76
9. Jamel MJ, Pereira Lde P, Mello NB, et al. Blood carbonyl protein measurement as a specific oxidative stres biomarker after intestinal reperfusion in rats. Acta Cir Bras. 2010;25(1):59-62
10. Maneewong K, Mekrungruangwong T, Luangaram S, et al. Combinatorial Determination of Ischemia Modified Albumin and Protein Carbonyl in the Diagnosis of Non ST-Elevation Myocardial Infarction. Ind J Clin Biochem. 2011;26(4):389-95
11. Pantke U, Volk T, Schumetzler M, et al. Oxidized proteins as a marker of oxidative stres during coronary heart surgery. Free Radic Biol Med. 1999;27(9-10):1080-6
12. Kavakli HS, Erel O, Delice O, et al. Oxidative stres increases in carbon monoxide poisoning patients. Hum Exp Toxicol. 2011;30(2):160-4
13. Wang P, Zeng T, Zhang CL, et al. Lipid peroxidation was involved in the memory impairment of carbonmonoxide-induced delayed neuron damage. Neurochem Res. 2009;34:1293-8
14. Garrabou G, Inoriza JM, Moren C, et al. Mitochondrial injury in human acute carbon monoxide poisoning: The effect of oxygen treatment. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2011;29(1):32-51
15. Alonso JR, Cardellach F, L´opez S, et al. Carbon monoxide specifically inhibits cytochrome c oxidase of human mitochondrial respiratory chain. Pharmacol Toxicol. 2003;93:142–6
16. Cardellach F, Alonso JR, L´opez S, et al. Effect of smoking cessation on mitochondrial respiratory chain function. J Toxicol Clin Toxicol. 2003;41:223–8
17. Mir´o O, Alonso JR, Jarreta D, et al. Smoking disturbs mitochondrial respiratory chain function and enhances lipid peroxidation on human circulating lymphocytes. Carcinogenesis. 1999;20:1331–6
18. Alonso JR, Cardellach F, Casademont J, et al. Reversible inhibition of mitochondrial complex IV activity in PBMC following acute smoking. Eur Respir J. 2004;23:214–8

ISSN: 1309-3878
Yayın Aralığı: Yılda 3 Sayı
Başlangıç: 2009
Yayıncı: Düzce Üniversitesi Tıp Fakültesi Aile Hekimliği AD adına Yrd.Doç.Dr.Cemil Işık Sönmez