Özlem ÖZDEN AKKAYA, Tayfun DİKMEN, Shah NAWAZ

Sığır Yağ Dokusundan İzole Edilen Mezenkimal Kök Hücrelerden Farklılaştırılan Nörosferlerde Sox2, ß-III Tubulin ve Nestin Ekspresyonlarının İmmunfloresan Boyama Yöntemi ile İncelenmesi

Kök hücre tedavisi, pek çok tedavisi olmayan hastalıkta olduğu gibi çoğu nörodejeneratif hastalık ve sendromda da daha iyi tedavi protokollerinin geliştirilmesi için önemli bir çalışma alanı haline gelmiştir. Kök hücrelerin klinik kullanımı pet hayvanları ve atlarda her ne kadar yaygınlaşmaya başlasa da sığırlarda kök hücre kullanımı hala oldukça sınırlıdır. Bu çalışmada sığır yağ dokusu kaynaklı mezenkimal kök hücreler izole edilmiş ve bu hücrelerden nörosfereldesi gerçekleştirilmiştir. Elde edilen nörosferlerin nöronspresifik belirteçler olan ß-III Tubulin, Nestin ve Sox2 ekspresyonları immunfloresan boyama tekniği ile değerlendirilmiştir. Çalışmadan elde edilecek sonuçlar sığır yağ dokusu kaynaklı mezenkimal kök hücrelerin nörojenik potansiyelinin daha iyi anlaşılması açısından önemli olmakla birlikte bu sonuçlar sığırlarda nörojenik defektlerde kullanılabilecek potansiyel tedavi yaklaşımlarının geliştirilmesi hususunda da yol gösterici olacaktır.

Anahtar Kelimeler:

Nestin, Nörosfer, Sığır, Sox2, Yağ Dokusu

Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining

Stem cell therapy can be an advantageous tool for non treatable diseases and has become a theme research for a better solution to many neurodegenerative cases. Despite of being very common in pet animals and equines in the field of veterinary medicine; stem cell based practises in bovines yet remain very limited. In the following study, Bovine adipose derived stem cells (BASCs) were isolated and differentiated into neurospheres. The potential expressions of neuron-specific markers β-III Tubulin, Nestin and Sox2 were demonstrated by immunofluorescence staining on these neurospheres. The outcomes of the study may provide a better understanding to the neurogenic potential of BASCs and can be a footstep for developing new therapeutical approaches for neurodegenerative diseases and syndroms in bovines.

Keywords:

Adipose, Bovine, Nestin, Neurosphere, Sox2,

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