Mandalardan Postmortem Elde Edilen Spermalarda Dondurmanın DNA Hasarı ve Bazı Spermatolojik Parametreler Üzerine Etkisi

Bu çalışmada, kesim sonrası manda testislerinden elde edilen epididimal spermada dondurma işleminin DNA hasarı ve bazı spermatolojik parametreler üzerindeki etkisinin araştırılması amaçlanmıştır. 50 adet erkek Anadolu mandasının (3 ve üzeri) testisleri mezbahanelerden kesim sonrası temin edildikten sonra elde edilen epididimal sperma progresif motilite (%), canlılık (%), anormal spermatozoa oranı (%) ve spermatozoon DNA hasarı (%) yönünden analiz edildi. Taze ve çözdürülmüş sperma arasında progresif motilite, canlılık, orta kısım, kuyruk ve toplam anormallik oranlarında önemli farklılıklar buldu (p<0,001). Taze ve çözdürülmüş spermada arasındaki DNA hasarı sonuçlarının anlamlı derecede farklı olduğu belirlendi (p<0,0001). Taze ve çözdürülmüş spermadaki progresif motilite oranları yapılan çalışmalarla uyumluydu. Çalışma sonucunda elde edilen veriler ışığında postmortem olarak elde edilen epididimal manda spermasının biyoteknolojik yöntemlerde kullanılabileceği düşünülmektedir. Bu araştırma, Anadolu manda popülasyonunun artırılmasına yönelik daha sonraki çalışmalara katkı sağlayacaktır. Spermatozoonlarda dondurma işlemi sonucunda şekillenen DNA hasarının belirlenmesi ve en aza indirilmesi için daha kapsamlı çalışmalar yapılmalıdır.

Anahtar Kelimeler:

Anadolu mandası, DNA hasarı, Epididimal, Sperma dondurma

Effect of Cryopreservation on DNA Damage and Various Sperm Parameters in the Post-Mortem Obtained Buffalo Sperm

This study aimed to investigate the effects of freezing on DNA damage and some sperm parameters in epididymal sperm obtained from the buffalo testicles after slaughtering. Epididymal semen examination of 50 male Anatolian buffaloes (three years old or older) was performed after being obtained from slaughterhouses. Sperm progressive motility (%), viability (%), abnormal sperm (%), and sperm DNA damage (%) were analyzed. The study found significant differences (p<0.001) between fresh and thawed semen on the progressive motility, viability, and mid-piece, tail, and total abnormality rates. It was determined that the difference between the DNA damage values in fresh and thawed semen was significant (p<0.0001). Progressive motility rates of fresh and thawed semen were consistent with the studies conducted. It is thought that epididymal buffalo semen obtained post-mortem can be used in biotechnological methods. This research will contribute to further studies to increase the Anatolian buffalo population. More comprehensive studies should be performed to determine the impact of fertility due to the semen DNA damage and minimize the DNA damage in the freezing-thawing semen samples.

Keywords:

Anatolian buffalo, DNA damage, Epididymal, Semen freezing,

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