Murat KAPLAN, Bahattin Taylan KOÇ, Kemal PEKMEZ, Abdurrahman Anıl ÇAĞIRGAN, Fatih ARSLAN

Türkiye’de Orf virusunun güncellenmiş moleküler karakterizasyonu

Orf virusu Türkiye'de koyun ve keçilerde endemiktir ve ilkbaharda artan oranlarda tespit edilmektedir. ORFV'nun ülkedeki bu önemli prevalans oranına rağmen, Türkiye'de bugüne kadar birkaç moleküler karakterizasyon çalışması yapılmış ve hepsi sadece B2L gen bölgesine odaklanmıştır. Bu çalışmanın amacı saha çalışmalarında izole edilen ORFV’nun farklı gen bölgelerinin moleküler karakterizasyonlarının belirlenmesidir. Çalışmada, 2018-2020 yıllarında Orf şüpheli yedi adet koyundan real-time PCR ile izole edilen pozitif ORFV izolatlarına ait farklı gen bölgelerinin (B2L, F1L ve VIR) kısmi genom dizilemesi yapıldı. Biyoenformatik çalışmalar ile moleküler karakterizasyonları belirlenerek GenBank'ta bulunan izolatlarla ilişkileri değerlendirildi. Moleküler ve in-siliko çalışmalardan sonra, her bir gen bölgesinin değerlendirilmesinde dikkate değer bir çeşitlilik tespit edildi. Bu sonuçlar, farklı varyantların ülkenin farklı bölgelerinde dolaşıma girmiş olma olasılığının yüksek olduğunu göstermiştir. Çalışma bir bütün olarak değerlendirildiğinde, moleküler çalışmalarda B2L gen bölgesinin yanı sıra F1L gen bölgesinin de dikkate alınması gerektiği düşünülmektedir. Hastalıkla ilgili korunma ve kontrol stratejisinin en iyi verimini sağlamak amacıyla ORFV'nun moleküler verileri güncellenmeli ve takip edilmelidir.

Anahtar Kelimeler:

Amino asit, filogeni, moleküler, orf virus, Türkiye

Updated molecular characterization of Orf virus in Türkiye

Orf (ORFV) virus is endemic in sheep and goats in Türkiye and detected with increasing incidence in Spring. Despite this significant prevalence rate of ORFV, a few molecular characterization studies have been conducted in Türkiye and all of them have focused on only B2L gene region so far. The aim of this study was to determine the molecular characterization of different gene regions of ORFV isolated from field studies. In the study, partial genome sequencing of different gene regions (B2L, F1L and VIR) of positive ORFV isolates detected by real-time PCR from seven sheep with suspected Orf in 2018-2020 were performed. Molecular characterizations were determined by bioinformatics studies. After the molecular and in-silico processes, we found remarkable diversification in the evaluation of each gene region. This result showed that different variants are more likely to have circulated in different parts of the Türkiye. When the study is evaluated as a whole, it is thought that the F1L gene region should be considered in addition to the B2L gene region in molecular studies. Molecular data of ORFV should be updated and followed to provide the best efficiency of the prevention and control strategy.

Keywords:

Amino acid, molecular, orf virus, phylogeny, Türkiye,

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