Kazeöz Lenfadenitisli Koyunlardan İzole Edilen Corynebacterium pseudotuberculosis Suşlarının in-Vitro Antibiyotik Duyarlılıkları

Koyun ve keçilerde Corynebacterium pseudotuberculosis tarafından oluşturulan kazeöz lenfadenitis (KLA), tüm dünyada yaygın olarak görülmekte ve önemli ekonomik kayıplara neden olmaktadır. Bu çalışmada, KLA’li koyunlardan izole edilen C. pseudotuberculosis suşlarının çeşitli antibiyotiklere in vitro duyarlılıklarının belirlenmesi amaçlandı. Çalışmada KLA lezyonları gösteren toplam 67 adet koyunun akciğerlerinden izole edilen 16 adet C. pseudotuberculosis suşu test edildi. Disk difüzyon yöntemiyle yapılan değerlendirmede; tetrasiklin, oksitetrasiklin, streptomisin, enrofloksasin, kloksasilin, ampisilin/sulbaktam, amoksisilin/klavulanik asit, penisilin/novobiosin, trimetoprim/sulfametaksazol ve neomisin/basitrasin/tetrasiklin diskleri kullanıldı. Suşların 14 (%87.5) adeti enrofloksasine, 13 (%81.2) adeti neomisin/basitrasin/tetrasikline, 12 (%75.0) adeti penisilin/novobiosine, 11 (%68.7) adeti oksitetrasikline, 11 (%68.7) adeti amoksisilin/klavulanik asite, 10 (%62.5) adeti kloksasiline, 9 (%56.2) adeti tetrasikline, 5 (%31.2) adeti ampisilin/sulbaktama ve 3 (%18.7) adeti ise trimetoprim/sulfametaksazole duyarlı bulundu. İzolatlardan hiçbiri streptomisine duyarlı bulunmadı.

Anahtar Kelimeler:

Antibiyotik duyarlılık testi, Corynebacterium pseudotuberculosis, Kazeöz lenfadenitis

In Vitro Antimicrobial Susceptibility of Corynebacterium pseudotuberculosis Isolated from Sheep with Caseous Lymphadenitis

Caseous lymphadenitis (CLA), caused by Corynebacterium pseudotuberculosis, is a highly prevalent disease of sheep and goats resulting in significant economic losses in the world. In this study, it was aimed to determine the in vitro antimicrobial susceptibility of C. pseudotuberculosis strains isolated from sheep with CLA to various antibiotics. A total of 16 C. pseudotuberculosis strains isolated from lung samples of 67 sheep with CLA lesions were tested. Tetracycline, oxytetracycline, streptomycin, enrofloxacin, cloxacillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, penicillin/novobiocin, trimethoprim/sulfamethoxazole and neomycin/bacitracin/tetracycline discs were tested by disc diffusion method. A total of 14 (87.5%) C. pseudotuberculosis strains were susceptible to enrofloxacin, 13 (81.2%) strains to neomycin/bacitracin/tetracycline, 12 (75.0%) strains to penicillin/novobiocin, 11 (68.7%) strains to oxytetracycline, 11 (68.7%) strains to amoxicillin/clavulanic acid, 10 (62.5%) strains to cloxacillin, 9 (56.2%) strains to tetracycline, 5 (31.2%) strains to ampicillin/sulbactam and 3 (18.7%) strains to trimethoprim/sulfamethoxazole. None of the isolates were susceptible to streptomycin.

Keywords:

Antimicrobial susceptibility testing, Caseous lymphadenitis, Corynebacterium pseudotuberculosis,

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