Mohammad Hossein RAZİ JALALİ, Sara LARKİ, Rahim PEYGHAN, Maryam BASTAMİ

İran'ın Güneybatısında Yer Alan Cyprinus Carpio ve Ctenopharyngodon İdella'daki Dactylogyrus Türlerinin Morfolojik ve Moleküler Tespiti

Çeşitlendirilmiş Dactylogyrus cinsinin solungaç monojenleri, Cyprinidae ailesinin tatlı su balıklarına ev sahipliğine özgüdür. Çeşitli Dactylogyrus türlerinin doğru tespiti çok zaman alıcı olduğundan ve çok deneyimli personel gerektirdiğinden, genomik DNA teknolojisi son zamanlarda Dactylogyrus spp. Bu çalışmada, Güneybatı İran'ın Ahvaz'ın farklı çiftliklerinden ve yerel pazarlarından 23 Cyprinus carpio ve 19 Ctenopharyngodon idella toplandı. Her balığın solungaç kemerleri, ışık mikroskobu altında dikkatle incelenir. Dactylogyrus türlerinin morfolojik olarak tanımlanması morfolojik olarak belirlenmiştir. benzer türler moleküler çalışma için etanol içinde tutuldu. Moleküler analiz, parazitin ribozomal RNA geninin ITS-1 bölgesinin amplifikasyonu kullanılarak yapıldı. Daha sonra amplifiye PCR ürünleri dizildi. Hizalanmış nükleotit sekansları, tanımlanan türlerin filogenetik ağacını yürütmek için analiz edilir. Sonuçlar, ortak sazandan izole edilen D. extensus ve çim sazan balıklarından alınan D. lamellatus da dahil olmak üzere iki soyun ortaya çıktığını göstermiştir. Filogenetik analiz, Khuzestan bölgesinden tespit edilen D. extensus ve D. lamellatus izolatlarının bazı İran (Guilan), Çin ve Çek Cumhuriyeti izolatlarıyla kümelendiğini gösterdi. Guilan Eyaletinden balıkların ithal edilmesi, yerli Cyprinid balıklarına monojenik enfeksiyonların ortaya çıkmasına neden olabilir.

Anahtar Kelimeler:

Sazan, Çim Sazan, Balık, PCR

Morphologic and Molecular Detection of Dactylogyrus Species in Cyprinus Carpio and Ctenopharyngodon Idella in Southwestern Iran

Monogeneans gill parasites of the diversified Dactylogyrus genus are host-specific to freshwater fish of the Cyprinidae family. Accurate detection of various Dactylogyrus species is very time-consuming and requires highly experienced personnel; to overcome this problem, the genomic DNA technology has been recently used to detect Dactylogyrus spp. In this study, 23 Cyprinus carpio and 19 Ctenopharyngodon idella isolates were collected from different farms and local markets of Ahvaz, southwest of Iran. The gill arches of each fish were carefully examined under a light microscope. Dactylogyrus species was identified morphologically. Identical species were kept in ethanol for molecular study. Molecular analysis was performed using amplification of the ITS-1 region of the ribosomal RNA gene of the parasite. Then, the amplified PCR products were sequenced. The aligned nucleotide sequences were analyzed to construct the phylogenetic tree of the identified species. The results revealed two lineages including D. extensus isolated from common carp and D. lamellatus taken from grass carp fish. Phylogenetic analysis showed that the detected D. extensus and D. lamellatus isolates from Khuzestan region were clustered with some Iranian (Guilan), Chinese and Czech isolates. Importing fingerlings from Guilan Province could lead to introducing monogenean infections to native cyprinid fish.

Keywords:

Dactylogyrus Common Carp, Grass carp, Fish, PCR,

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