Tahir KAHRAMAN, Ramazan ÜSTÜN, Fahri BAYIROĞLU, Recep ASLAN

Gıda Kısıtlamasının Ratlarda Doku Lipid Peroksidasyonu ve Glutatyon Düzeylerine Etkilerinin Araştırılması

Gıda kısıtlamasının metabolik hızı azaltarak, lipid peroksidasyonu düşürdüğü öne sürülmektedir. Oksijen metabolizmasının bu ara indirgenme ürünlerinin düzeyleri, hücresel savunma mekanizmasını oluşturan antioksidan sistemler ile kontrol edilmektedir. Kısıtlı beslemenin antioksidan savunma sistemini destekleyip güçlendirdiği de bildirilmektedir. Araştırmada 8 hafta süreyle % 50 gıda kısıtlaması uygulanan 6 haftalık erkek Wistar Albino ratların karaciğer, beyin, testis, dalak, kalp ve böbrek dokularında; lipid peroksidasyon ürünü malondialdehid (MDA) ve glutatyon (GSH) düzeylerindeki değişimler incelenmiştir. Gıda kısıtlaması sonrası deneme grubu, doku MDA düzeyinde artış gözlenmesine karşın bu durum istatistiksel açıdan önemli bulunmamıştır (p>0.05). Deneme grubu GSH düzeylerinde, karaciğer, beyin ve böbreklerde bir miktar azalma olmasına karşın testis dokusunda hafif artış, dalak ve kalp dokusunda herhangi bir değişim gözlenmemiştir. Kontrol ve deneme grubu GSH düzeyleri, istatistiksel açıdan önemli bulunmamıştır (p>0.05). MDA düzeylerinde hafif artışın gözlenmesi, buna karşın GSH düzeylerinde hafif azalmanın tespit edilmesini diyet kısıtlaması sırasında protein alımının yetersizliği ve homeostazisin sağlanması için adaptasyon amacıyla gerçekleştiği düşünülmektedir

Anahtar Kelimeler:

Tissue

The Effect of the Food Restriction on Lipid Peroxidation and Glutathione Levels in Rat Tissues

Food restriction is known to decrease lipid peroxidation by causing reduced metabolic rate. During the oxygen metabolism in the body, lipid peroxidation end-products are generated. It is suggested that food restriction causes the enhancement of the antioxidant system. In this study, the alterations of malondialdehid which is one of the end-products of the lipid peroxidation and glutathione levels in the tissues liver, brain, testis, spleen, heart and kidney of the rats which exposed to the food restriction by 50 % during 8 weeks were investigasted. After the restriction period, even though the tissue MDA levels of the experimental group was found to be increased, this was not statistically important (p>0.05). As for the GSH levels of the study group, it tended to decrease in the liver, brain and kidneys but slightly increased in the testis tissue while there were no change in the spleen and heart tissues in respect of GSH levels. In spite of these alterations, it was not found statistical importance between the study and the control groups (p>0.05). It can be concluded that some adaptational changes such as slight increase in MDA levels and decrease in GSH levels started to take place in response of decreased protein and energy intake. But we think that the period of restriction was not enough to observe the drastical changes in these mentioned parameters in tissue levels

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