F64A TARAFINDAN ALZHEİMER BENZERİ DEMANS OLUŞTURULAN RATLARDA BETAİN ve PİPERİNİN ETKİSİ
AMAÇ: Demans hastalarının büyük çoğunluğu (yaklaşık %60- 70) Alzheimer hastalığı (AH)’ndan muzdariptir. AH’nın ayırt edici patolojik belirtileri senil plaklar (SP'ler), nörofibriler yumaklar (NFTS), ve nörodejenerasyondur. Bu çalışmada; nörolojik anomalileri indükleyen Acetylcholine Mustard Aziridin İon (AF64A)'nın neden olduğu hasar ve bu zararın antioksidan piperin ve betain’nin tedavi edici etkisinin belirlenmesi amaçlanmıştır. GEREÇ VE YÖNTEM: Bu çalışmada; 24 Sprague-Dawley erkek sıçan kullanılmış ve 4 grup oluşturulmuştur: Sağlıklı sıçanlardan oluşan grup 1 (kontrol, n=6); AF64A ile deneysel demans oluşturulan grup 2 (n=6), betain tedavisi yapılan grup 3 (AF64A betain, n=6) ve piperin tedavisi yapılan grup 4 (AF64A piperin, n=6). Hipokampus dokusunda mitojenle aktieşmiş protein kinaz-1(MAPK-1) mRNA düzeyi, karaciğer ve kan serum örneklerinde Malondialdehit (MDA) düzeyleri ile karaciğer ve eritrosit örneklerinde redükte glutatyon (GSH) düzeyleri araştırılmıştır. İlaveten, morris su labirent testi kullanılarak davranışsal yönden kaynaklanan farklılıklar süre açısından belirlenmiştir. BULGULAR: Karaciğer ve eritrositlerdeki en yüksek GSH düzeyleri piperin uygulanan grup 4’de belirlenmiştir (p
THE EFFECT OF BETAINE AND PIPERINE ON RATS WITH CREATED ALZHEIMER- LIKE DEMENTIA BY AF64A
OBJECTIVE: The great majority of dementia patients (about 60- 70%) suer from Alzheimer disease (AD). The distinctive pathological signs of AD are senile plaques (SPs), neurofibrillary tangles (NFTS), synaptic loss and neurodegeneration. In this study; it is aimed to determine the damage caused by Acetylcholine Mustard Aziridin Ion (AF64A), which induces neurological anomalies, and the therapeutic eect of antioxidant piperine and betaine. MATERIAL AND METHODS: In this study; 24 Sprague-Dawley male rats were used and 4 groups were formed: Group 1 consisting of healthy rats (control, n = 6); Group 2 (n = 6) with experimental dementia induced by AF64A, group 3 (AF64A betaine, n = 6) treated with betaine and 4 (AF64A piperine, n = 6) treated with piperine. The mRNA levels of mitogen activated protein kinase-1 (MAPK-1) in hippocampus tissue, Malondialdehyde (MDA) levels in liver and blood serum samples and reduced glutathione (GSH) levels in liver and erythrocyte samples were investigated. In addition, behavioral dierences were determined in terms of duration using the morris water maze test. RESULTS: The highest GSH levels in liver and erythrocytes were determined in piperine-treated group 4 (p
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- ISSN: 1302-4612
- Yayın Aralığı: Yılda 4 Sayı
- Başlangıç: 1999
Sayıdaki Diğer Makaleler
İNSİDENTAL ADRENAL LEZYONLARIN KLİNİK, HORMONAL VE HİSTOLOJİK DEĞERLENDİRİLMESİ: TEK MERKEZ SONUÇLAR
Şafak AKIN, Selçuk DAĞDELEN, Okan Bülent YILDIZ, Alper GÜRLEK, Tomris ERBAŞ, Miyase BAYRAKTAR
F64A TARAFINDAN ALZHEİMER BENZERİ DEMANS OLUŞTURULAN RATLARDA BETAİN ve PİPERİNİN ETKİSİ