MUSTAFA SOLAK, Bilal GÜMÜŞ, Tevhide FISTIK

Cytogenetic analysis in infertile men

ISSN: 1302-4612
Yayın Aralığı: Yılda 4 Sayı
Başlangıç: 1999

Anahtar Kelimeler:

Sitogenetik analiz, Kısırlık, erkek, Kromozom anomalileri

In this report, we present cytogenetic studies in primary infertile men. There are many ethiologic factors in male infertility. Chromosomal abnormalities are involved in these factors. Conventional cytogenetic and molecular cytogenetic analyses of infertile male provide us important information. Primary infertile 148 cases who have applied to infertility out patient department participated in this study. The history of each case was taken and physical exalirnation was done. Hormonal profiles scrotal USG and spermiogram was made all cases and testicular biopsy was performed to the ones who has indications. Afterwards, 28 primarily infertile cases who have oligo/azospermia, normal hormonal profile and minimal FSH and although a medical treatment exist cases still having spermiogram parameters unchanged; cytogenetic analysis were done. The mean age of cases was 34 and the mean of their sperm levels was 8.02 million per ml. Chromosome analysis was performed on phytohaemaglutinin stimulated lymphocytes from cases according to standard cytogenetic technique. Metaphase spreads were stained using GTG and C banding techniques. In order to clarify a derivative chromosome Fluorescence in situ hybridisation (FISH) was applied to metaphase preparation. At the end of cytogenetic studies; we have observed normal (46,XY) karyotype in 24 of 28 (85.7 %) cases; in three cases (10.5 %) 47, XXY and in one case (3.5 %) 46 ,X, i (Yp karyotype. So, totally 4 cases (14.3%) have chromosomal disorders. In our opinion; the rate of chromosomal disorders in infertile males is important. Conventional and molecular cytogenetic studies can uncover chromosomal disorders in infertile men.

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