Deniz DİNÇEL, Hale ŞAMLI, Ümit İNCE, Asuman ÖZGÖZ, Murat ŞAMLI, Ahmet ŞAHİN

FGFR1 SER777 Mutasyonu ve Mesane Kanseri

Amaç: ERK 1/2 (ekstraselüler sinyalle düzenlenen kinaz 1, 2) ve p38α’nın, FGFR1 Ser 777’yi fosforile etmek yoluyla anti-tümörojenik etki göstermesi muhtemeldir. Ser777 fosforilasyonunun ise FGFR1 aktivasyonunu inhibe ettiği bilinmektedir. Dolayısıyla Ser777’nin mutasyona uğramasının FGFR1’i aktive ederek; karsinogenez ile bunu devam ettirebilecek mekanizmaları harekete geçirebileceği düşünülmüştür. Bu kapsamda gerçekleştiren çalışmada; mesane kanseri ile FGFR1 Ser777 mutasyonu arasındaki ilişkinin tespiti amaçlanmıştır. Yöntem: Çalışmada; mesane kanseri tümör dokusu (n=62) ve kontrol grubu örneklerinde (n=30) DNA izolasyonunun ardından, FGFR1 Ser777 mutasyon analizi, DNA dizi analizi yöntemi ile gerçekleştirilmiştir. Bulgular: Bildiğimiz kadarıyla ilk defa çalışılan söz konusu mutasyon, incelenen hasta ve kontrol grubu örneklerinde tespit edilememiştir. Sonuç: Elde edilen bulgular, Ser777’nin mesane kanserinde mutasyona uğramadığını düşündürmüştür. Literatürde konuyla ilgili başka çalışma bulunmadığı göz önüne alındığında, çalışma sonuçlarının literatüre önemli katkı sağlayabileceği düşünülmektedir. Dahası, diğer kanser türlerinde de FGRF1 Ser777 mutasyonunun çalışılmasının farklı bakış açıları ortaya koyabileceğini düşünmekteyiz.

Anahtar Kelimeler:

Dizi analizi, FGFR1, Ser777, Mesane Kanseri

FGFR1 SER777 Mutation and Bladder Cancer

Objective: The ERK 1/2 (extracellular signal-regulated kinase 1, 2) and p38α probably have an anti-tumorigenic effect by phosphorylating FGFR1 Ser 777. The Ser777 phosphorylation is known to inhibit FGFR1 activation. Therefore, the Ser777 mutation was suggested to trigger the carcinogenesis, and the other continued mechanisms by activating the FGFR were suggested. We aimed to determine the relationship between FGFR1 Ser777 mutation and bladder cancer were aimed to be determined in this context. Methods: After the DNA isolation of bladder cancer tumor tissues (n = 62) and control group samples (n = 30), the DNA sequence analysis was performed for the FGFR1 Ser777 mutation in the study carried out for this purpose. Results: The mutation which has been studied for the first time to the best of our knowledge was not detected in the patient and control group samples in the current study. Conclusion: As a conclusion the data suggested that Ser777 did not mutate in bladder cancer. Considering that there are no other studies on the subject in the literature, it is thought that the results of the study might make a significant contribution to the literature. Moreover, in our opinion, studying the FGRF1 Ser777 mutation in the other types of the cancer will reveal different points of view.

Keywords:

Sequence analysis, FGFR1, Bladder cancer, Ser777,

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