Defne GÜMÜŞ, Fatma KALAYCI YÜKSEK, Mine ANĞ KÜÇÜKER, Gül İpek GÜNDOĞAN

Farklı Hormonların ve Hücre Hatlarının Bir Üropatojen E. Coli Suşunun Üremesi Üzerine Etkileri

GİRİŞ ve AMAÇ: Bir infeksiyon sürecinde patojenlerin önemli biyolojik özelliklerinin konak faktörleri tarafından etkilendiği bilinmektedir. Bu çalışmada, çeşitli memeli hormonlarının farklı hücre hatları varlığında bir üropatojen E. coli (UPEC) suşunun üremesi üzerine olası etkileri araştırılmıştır. YÖNTEM ve GEREÇLER: Hücre hatlarının (HEK293, A549, L929, Ishikawa) kültürü yapılmıştır. Fizyolojik seviyelerine göre her bir hormonun iki farklı konsantrasyonu (20 ve 200µIU insulin; 0,0017 ve 0,04µg/mL norepinefrin; 0,1 ve 0,4ng/mL östradiol; 2 ve 20ng/mL progesteron; 6 ve 60pg/mL melatonin) deneylerde kullanılmıştır. Bakteri ve hormonlar her bir hücre kültürüne inoküle edilmiştir. Üremeler, üç saatlik inkübasyon sonucunda 600nm’de absorbans ölçümü ile belirlenmiştir. BULGULAR: UPEC suşunun üremesi yüksek düzey östradiol, yüksek düzey norepinefrin ve her iki düzey melatonin varlığında anlamlı derecede azalmıştır (p

The Alterations on Growth of A Uropathogenic E. Coli with The Effects of Both Different Hormones and Cell Lines

INTRODUCTION: It is well known that, biologicallyimportant processes of pathogens can be affected from hostfactors during infectious processes. We aimed to investigatethe possible effects of various mammalian hormones indifferent cell lines on growth of a uropathogenic E. coli(UPEC) strain.METHODS: Cell monolayers (HEK293, A549, L929,Ishikawa) were cultivated. Two different concentrations ofeach hormone were analyzed (20 and 200µIU insulin; 0.0017and 0.04µg/mL norepinephrine; 0.1 and 0.4ng/mL estradiol; 2and 20ng/mL progesterone; 6 and 60pg/mL melatonin)according to their physiological levels. Bacterium andhormones were inoculated onto each cell cultures. After threehours incubation growths were determined by optical densitymeasurement at 600 nm.RESULTS: Growth of UPEC has been decreased significantlyin the presence of high level estradiol, high levelnorepinephrine and all levels melatonin (p< 0.05). Besides,HEK293 and Ishikawa cell lines were found to be the mosteffective on growth of pathogen in the presence of differenthormones. The coexistence of Ishikawa and all types ofhormones except progesterone were shown to be enhanced thegrowth of UPEC (p< 0.05). The coexistence ofHEK293+insulin and HEK293+estradiol reduced the growthof UPEC; whereas HEK293+melatonin andHEK293+progesteron enhanced the growth significantly (p

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