Burak COBAN, Cengiz SAĞLAM, Nursel ESER, İlknur BABAHAN

Tiyosemikarbazon İçeren Vis-Dioksim Ligandının Ni II Kompleksinin DNA Etkileşimlerinin İncelenmesi

Vis-dioksim ligantları biyolojik aktiviteleri açısından çok önemlidir ve metal kompleksleri yoğun bir şekilde çalışılmıştır. Ayrıca birçok tiyosemikarbazon türevi de sentezlenmiş ve potansiyel anti-tümör ajanları olarak rapor edilmiştir. DNA’ya bağlanma çalışmalarında kullanılan çeşitli metal kompleksleri arasında Nikel II kompleksleri de yer almaktadır. Bütün bu özellikleri visdioksim, tiyosemikarbazon grubu ve Ni II bir arada bulunduran bileşikler literatürde DNA’ya bağlanması açısından daha evvel hiç değerlendirilmemiştir. Tiyosemikarbazon içeren vis-dioksim ligandının Ni II kompleksinin DNA ile etkileşimleri UV titrasyon yöntemi, flouresans, ve agaroz jel elektroforez yöntemleri ile ilk defa çalışılmıştır

Anahtar Kelimeler:

DNA, İnterkalasyon, Nikel II kompleksi, Tiyosemikarbazon, Vis-dioksim

Studying DNA interactions of Ni II complexes of thiosemicarbazone containing vic-dioxime ligands

Vic-dioxime ligands and their metal complexes are very important for their biological activities and thoroughly studied. In addition, several thiosemicarbazone derivatives were synthesized and their potentials as anti-tumor agents were reported. As well as this, Nickel II complexes, among the other metal complexes, have an important proportion in DNA interaction studies. The compounds which have all these features vic-dioxime, thiosemicarbazone group and Ni II are not evaluated for their interactions with DNA.DNA interactions of Ni II complex of thiosemicarbazone containing vic-dioxime ligand will be studied by using UV titration, floresans, and agaroz gel electrophoresis methods for the first time.

Keywords:

Nickel II complex, Thiosemicarbazone, vic-dioxime, DNA, Intercalation,

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