Yasin ÖZTÜRK, Sadettin TANYILDIZI, Fatih Ahmet KORKAK, Burcu GUL BAYKALIR, Zeliha KESKİN, Betul DAGOGLU HARK, Gurdal DAGOGLU

Xanthium strumarium’da Atraktilozid Seviyeleri ve Xanthium strumarium Verilen Sıçanların Serumunda Atraktilozid Konsantrasyonları

Atraktilozid (ATR) karaciğer ve böbrekte ciddi hasarlar yapabilen bir toksindir. Buna karşın bileşiğin kandaki dağılımı ve kinetiği hakkındaki bilgiler yetersizdir. Bu çalışmada gas kromatografi-kütle spektrofotometre (GC-MS) ile Xanthium strumarium’daki atraktilozid seviyelerini, serum konsantrasyonunu ve zamana bağlı bazı farmakokinetik parametrelerini belirledik. Sıçanlara (80 mg/kg ATR) X. strumarium tohum ekstresinin mide sondası ile uygulanmasından sonra 0, 4, 6, 8, 12, 24, 36, 48, 60, 72, 96, ve 120. saatlerde kan alındı. Serum ve bitki örneklerinin ekstraksiyonu, hidrolizi ve türevlendirilmesi yapıldıktan sonra GC-MS cihazı ile analizi yapıldı. X. strumarium tohumlarında ATR seviyesi Ağustos’ta 3.043 mg/g, Eylül’de 3.502 mg/g ve Ekim’de 3.800 mg/g saptanmıştır. Bitkinin diğer kısımlarında ATR saptanmamıştır. ATR’nin Cmax değeri 10.77 µg/mL, Tmax 48 saat ve t1/2 6.07 saat olarak hesaplandı. Kandaki ATR seviyesinin dağılımının bilinmesi zehirlenmelerde semptomların başlangıcını, toksik etkilerin seyrini ve sağaltım planını belirlemeye yardımcı olacaktır. Ayrıca, bu çalışmadan elde edilen bulgular, ATR zehirlenmesi ile ilgili antidot çalışmalarına katkı sağlayacaktır.

Atractyloside Levels in Xanthium strumarium and Atractyloside Concentrations in the Serum of Rats Given Xanthium strumarium

Atractyloside (ATR) may cause severe liver and kidney damage. However, there is a lack of information about levels of ATR in parts of Xanthium strumarium and the concentrations and kinetics of ATR in the serum. In this study, we determined atractyloside levels in Xanthium strumarium parts, its serum concentrations and some kinetic parameters concerning time with the gas chromatography-mass selective (GCMS) method. After administering (80 mg/kg ATR) X. strumarium seed extract to rats through gastric gavage, blood samples were collected at the 0th, 4th, 6th, 8th, 12th, 24th, 36th, 48th, 60th, 72nd, 96th, and 120th h. After extraction, hydrolysis and derivatization of the plant and serum samples were analyzed by the GC-MS instrument. The ATR level in the X. strumarium seeds was 3.043 mg/g in August, 3.502 mg/g in September and 3.800 mg/g in October. ATR was not detected in other parts of the plant. After ATR administration to rats, the Cmax value of ATR was calculated as 10.77 µg/mL at Tmax of 48 h and t1/2 of 6.07 h. A thorough understanding of ATR circulation in the blood will aid in determining the course of its toxic effects in the bloodstream, the onset of symptoms and the general management plan for ATR poisoning. Moreover, the results obtained from this study will contribute to the antidote studies for ATR poisoning.

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