Cem OZIC, Muhammed YAYLA, Pınar AKSU KILICLE, Nevra AYDEMIR CELEP, Ahmet HARMANKAYA, Levent SAHIN, Erdem TOKTAY, Serdar YIGIT

Ejder Meyvesi Ekstraktının Sıçanlarda Deneysel Mezenter Arteriyel İskemi-Reperfüzyon Üzerine Etkisi

Ejder meyvesi ekstraktının (EME), sıçan bağırsağında iskemi ve reperfüzyon hasarına karşı gelişen oksidatif stres hasarında koruyucu bir ajan olabileceğini varsaydık. Çalışmada kullanılan sıçanlar her grupta 6 hayvan olacak şekilde rastgele 8 gruba ayrıldı. SAĞLIKLI grubu, iskemiyle indüklenmedi ve EME verilmedi (Grup 1). EME1000 grubu, iskemiyle indüklenmedi; ancak EME 1000 mg/kg verildi (Grup 2). Grup 3, 4, 5, 6, 7 ve 8’de iskemi 1 saat süreyle indüklendi. Daha sonra 45 dakika reperfüzyona izin vermek için klempler çıkarıldı (Grup 6, 7 ve 8). Sıçanlara İskemi (I) ve İskemi/Reperfüzyon (I/R) uygulamasından 30 dakika önce 500 mg/kg ve 1000 mg/kg dozlarda EME verildi. Deney sonunda bağırsak dokularında histopatolojik, biyokimyasal ve moleküler analizler yapıldı. Glutatyon peroksidaz, süperoksit dismutaz, glutatyon seviyeleri I + EME500, I + EME1000, I/R + EME500 ve I/R + EME1000 gruplarında I ve I/R gruplarına göre önemli ölçüde artarken, tümör nekroz faktörü-α, Kaspaz 3 ve malondialdehit seviyelerinde anlamlı azalma oldu (P

The Effect of Dragon Fruit Extract on Experimental Mesentery Arterial Ischemia-Reperfusion in Rats

We hypothesized that dragon fruit extract (DFE) might be a protective agent in oxidative stress damage that develops against ischemia and reperfusion injury in the rat intestine. The rats used in the study were randomly divided into 8 groups, with 6 animals in each group. The HEALTHY group was not induced by ischemia and not given DFE (Group 1). The DFE1000 group was not induced by ischemia; however, DFE was given 1000 mg/kg (Group 2). Ischemia was induced for 1 h in groups 3, 4, 5, 6, 7 and 8. The clamps were then removed to allow reperfusion for 45 min (Groups 6, 7 and 8). DFE was given to rats at doses of 500mg/kg and 1000 mg/kg 30 min before Ischemia (I) and Ischemia/Reperfusion (I/R) administration. At the end of the experiment, histopathological, biochemical and molecular analyses were performed on the intestinal tissues. While glutathione peroxidase, superoxide dismutase, glutathione levels increased significantly in the I+DFE500, I+DFE1000, I/R+DFE500 and I/ R+DFE1000 groups compared to the I and I/R groups, there was a significant decrease in tumor necrosis factor-α, Caspase 3 and malondialdehyde (P

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