Rao Zahid ABBAS, Zurisha RANI, Asghar ABBAS, Zohaib SAEED, Tauseef ur REHMAN, Riaz HUSSAIN, Khalid MEHMOOD, Atif REHMAN, Kashif HUSSAIN

Butirik Asitin Etlik Piliçlerde In Vitro ve In Vivo Antikoksidiyal Etkinlikleri ve Kan ve Serum Kimyası Üzerine Etkisi

Bu çalışmada, bütirik asitin antikoksidiyal aktivitesinin in vitro ve in vivo yöntemlerle değerlendirilmesi amaçlandı. Bütirik asitin, koksidian ookistlerin sporülasyonu ve hasarı üzerine etkisini incelemek için in vitro sporülasyon inhibisyon testi yapıldı. Çeşitli konsantrasyonlarda bütirik asit uygulanması sporülasyon inhibisyonuna neden olurken, doza bağlı bir şekilde koksidian ookistlere zarar verdi. In vivo denemeler için 72 adet bir günlük etlik civciv, her grupta eşit (n=12) civciv olacak şekilde rastgele 6 gruba, yani A, B, C, D, E ve F’ye ayrıldı. Bir haftalık uyum aşamasından sonra grup A, B ve C’ye sırasıyla %1.2, %1 ve %0.8 bütirik asit verilir iken, grup D pozitif kontrol (enfekte ve ilaçlı), grup E negatif kontrol (enfekte ve ilaçsız) ve grup F normal kontrol (enfekte olmayan) grubu olarak değerlendirildi. Aynı gün, tedavi edilen tüm gruplar, Eimeria tenella’ya ait 50.000 sporlu ookist ile oral yolla enfekte edildi. Sonuçlar, bütirik asit uygulamasının piliçlerin kilo alımı ve FCR gibi performans ve lezyon ve ookist skoru ve gram başına ookist miktarı gibi antikoksidiyal parametreler üzerine olumlu etkilere yol açtığını ortaya koydu. Bütirik asit ayrıca broyler piliçlerin hematolojik değerlerini ve serum kimyasını da iyileştirdi.

In Vitro and In Vivo Anticoccidial Effects of Butyric Acid and Its Impact on Blood and Serum Chemistry of Broiler Chickens

Present study was aimed to evaluate the anticoccidial activity of butyric acid by both in vitro and in vivo methods. In vitro trial was conducted by sporulation inhibition assay to examine the influence of butyric acid on sporulation and damage of coccidian oocysts. Administration of various concentrations of butyric acid induced sporulation inhibition and also damaged coccidian oocysts in dose dependent manner. For in vivo trials, 72 (day-old) broiler chicks were randomly divided into 6 groups i.e., A, B, C, D, E and F having equal chicks in each group (n=12). After one week of acclimatization, three doses of butyric acid 1.2%, 1% and 0.8% were given to group A, B and C, respectively while group D was named as positive control (infected medicated), group E was named as negative control (infected and non-medicated) and group F served as normal control (non-infected) group. On the same day, all treated groups were orally infected with 50.000 sporulated oocysts of Eimeria tenella. Results revealed that administration of butyric acid induced positive effect on chicken’s performance such as weight gain, FCR and anticoccidial parameters like lesion and oocysts score, oocyst per gram. Butyric acid also improved hematological values and serum chemistry of broiler chicken.

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