MURAT SIRRI AKOSMAN, Vural ÖZDEMİR, HASAN HÜSEYİN DEMİREL, Johnnie Bremholm ANDERSEN

Unbiased estimate of the Epithelial cell number and Epithelial cell nuclear volume in the bulbourethral (Cowper) glands of Holstein bulls - A stereological study

Glandula bulbourethralis’in fertilitedeki önemli rolünden dolayı bu eklenti bezinin sağlıklı hayvanlarda morfolojik ve histomorfolojik yapısının sayısal değerleri önemlidir. Bu çalışmada, Holştayn ırkı boğalardan alınan gl. bulbourethralis’lerin hacim, toplam epitel hücre sayısı ve epitel hücre çekirdeklerinin hacimleri ilk kez stereolojik metotlar kullanılarak hesaplandı. Epitel hücre sayımı için smooth fractionator tekniği kullanıldı. Epitel hücrelerin çekirdek hacimleri vertikal kesitler ile saptandı. Epitel hücrelerinin sayısı 322x107,(CE:0,1) ve epitel hücre çekirdek hacmi 59.1 μm3 (SD=3.7) olarak hesaplandı. Elde edilen bulguların CE ve SD değerleri normal sınırlar içinde olduğu görüldü.

Anahtar Kelimeler:

erkek üreme organları, boğa, süt sığırı, hayvan anatomisi, doku bilimi, Alacalı Sığır, erkek döl verimi, sığır, morfoloji

Holştayn boğalarda bulbourethral (Cowper) bezin epitel hücre sayısı ve epitel hücre çekirdeği hacminin hesaplanması - stereolojik çalışma

The bulbourethral gland (or Cowper’s gland) plays an important role for fertility. It is, therefore, essential to have quantitative data about the morphological and histomorphological structure of this gland in nonpathological conditions. In the present study, Holstein bull’s bulbourethral glands were collected, and the volume of the glands, total epithelial cell number and epithelial cell’s nuclear volume was estimated for the first time by stereological methods. The smooth fractionator technique for epithelial cell counting was used. Epithelial cell’s nuclear volume was estimated on vertical sections. The mean number of the epithelial cells (coefficient of error; CE) and the mean epithelial cell’s nuclear volume were found 322x107 (0.1) and 59.1 μm3 (SD=3.7) respectively. Finally, the present studies stereological findings are in the acceptable range.

Keywords:

male genitalia, bulls, dairy cattle, animal anatomy, histology, Holstein-Friesian, male fertility, cattle, morphology,

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