METİN GÜRÇAY, TURHAN TURAN, Ayşe PARMAKSIZ

Türkiye’de kültürü yapılan Gökkuşağı alabalıklarında (oncorhynchus mykiss walbaum, 1792) infeksiyöz pankreatik nekrozis virus varlığının tespiti üzerine bir araştırma

Bu çalışmada, Türkiye’nin farklı bölgelerindeki gökkuşağı alabalık çiftliklerinde infeksiyöz pankreatik nekroz virusunun (IPNV) varlığı araştırılmıştır. Bu amaçla, Aralık 2010 ile Mart 2011 tarihleri arasında, 30 alabalık çiftliğindeki anaçlardan 47 seminal sıvı, 62 ovarial sıvı alınmış ve 134 yavru alabalıktan oluşan toplam 243 izolasyon materyali temin edilmiştir. Virus izolasyonu için izolasyon materyallerinin BF-2 (Bluegill fry-2) hücresinde iki pasajı yapılmıştır. Sitopatik effekt (SPE) gösteren hücre kültür süpernatantları, IPNV identifikasyonu için antijen enzyme linked immunosorbent assay (Ag-ELİSA) metodu ile analize tabi tutulmuştur. Bütün izolasyon materyallerine, hücre kültürü izolasyonu testiyle karşılaştırmak amacıyla Ters transkriptaz-polimeraz zincir reaksiyonu (RT-PZR) testi de uygulanmıştır. Toplam 243 izolasyon materyalinin 26’sından (%10.7) IPNV izole edilmiştir. İzolasyon materyali alınan 11 ilin 7’sinde (%63.6) ve toplam 30 çitliğin 17’sinde (%56.6) IPNV varlığı tespit edildi. Ayrıca, virus izolasyon yöntemine göre daha ucuz ve daha hızlı bir metot olan RT-PZR testinin, IPNV’nun direkt dokudan tanısında alternatif bir metot olarak kullanılabileceği belirlenmiştir.

Anahtar Kelimeler:

seminal plazma, ELISA, Türkiye, sucul kültür, sucul hayvanlar, sucul organizmalar, sitopatojenite, teşhis, tanı teknikleri, kültür balığı, balık hastalıkları, yumurtalıklar, gökkuşağı alabalığı, viral hastalıklar

A study on the presence of infectious pancreatic necrosis virus infections in farmed rainbow trout (oncorhynchus mykiss walbaum, 1792) in Turkey

In this study, the presence of infectious pancreatic necrosis virus (IPNV) was investigated in farmed rainbow trout in different regions of Turkey. For this purpose, total of 243 isolation materials (47 seminal fluids, 62 ovarian fluids and 134 fingerling) were collected from 30 farms in the period of December and March 2010-2011. Isolation materials were passaged twice in BF-2 (Bluegill fry-2) cell cultures for virus isolation. The cell culture supernatants showed cytopathic effect (CPE) were tested by antigen-capture enzyme linked immunosorbent assay (Ag-ELISA) for IPNV identification. All isolation materials were tested also by reverse transcriptase polymerase cahin reaction (RT-PCR) in order to compare with the test of the virüs isolation. IPNV was detected from 26 (10.7%) of total 243 isolation materials obtained from 17 (56.6%) of 30 farms in 7 (63.6%) of 11 provinces. In addition, that RT-PCR test can be used as an alternative method in the IPNV-diagnosis by directly tissue-testing due to less expensive and faster than the virus isolation method was determined.

Keywords:

seminal plasma, ELISA, Turkey, aquaculture, aquatic animals, aquatic organisms, cytopathogenicity, diagnosis, diagnostic techniques, fish culture, fish diseases, ovaries, rainbow trout, viral diseases,

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