TİMUR GÜLHAN, BANUR BOYNUKARA, MUSTAFA ALİŞARLI

Typing of verotoxigenic Escherichia coli strains isolated from animal and human sources

Bu çalışmada Vero hücre kültürü tekniği ile verotoksijenik olduğu bilinen hayvan kaynaklı 58, insan kaynaklı 15, toplam 73 E. coli suşunun Verotoksin tipleri (VT1, VT2) araştırıldı. Tiplendirme Duopath verotoksin GLISA test kiti kullanılarak yapıldı. Bu suşların 31’i (%42.5) VT1, 20’si (%27.4) VT2, 16’sP (%21.9) VT1+VT2 olmak üzere 67 tanesi (%91.8) tiplendirildi. İnsan kaynaklı 1, hayvan kaynaklı 5, toplam 6 suş (%8.2) tiplendirilemedi. İnsan ve gıda kaynaklı E. coli suşlarının verotoksin tiplerinin belirlenmesinde kullanPlan bu tekniğin, hayvan orijinli verotoksin tiplerinin belirlenmesinde de pratik bir test olarak kullanabileceği görüldü.

Anahtar Kelimeler:

hayvanlar, soy, insan, izolasyon, saptama, bakteriyel toksinler, tipler, verotoksinler, Escherichia coli

Hayvan ve İnsanlardan izole edilen verotoksijenik Escherichia coli suşlarının tiplendirilmesi

In this study, the verotoxin types of 73 E. coli strains, 58 animal origin and 15 human origin, were examined by Vero cell culture technique. Typing was performed using Duopath verotoxin GLISA test tool. Of them 67 (91.8%) strains were typed; 31 (42.5%) were VT1, 20 (27.4%) were VT2 and 16 (21.9%) were VT1+VT2. 1 animal origin and 5 human origin totally 6 (8.2%) strains could not be typed. It was seen that this technique used in determining the verotoxin types of human and food origin E. coli strains of can also be used as practical test in determining the verotoxin types of animal origin E. coli strains.

Keywords:

animals, strains, man, isolation, detection, bacterial toxins, types, verotoxins, Escherichia coli,

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