SİBEL HASIRCIOĞLU, MEHMET KALE, ABUZER ACAR

Investigation of pestivirus ınfections in aborted sheep and goats in Burdur region

Bu çalışmada, koyun ve keçilerde abort olgularının önemli bir nedeni olarak bildirilen pestivirus varlığı abort yapmış koyun ve keçilerde serolojik ve virolojik yönden araştırıldı. Bu amaçla abort yapmUş 735 adet koyun, 35 adet keçiden serum ve lökosit örnekleri ve 48 adet abort / ölü doğmuş kuzudan doku örnekleri toplandı. Bu örnekler pestivirus antijen ve antikor varlığı yönünden ELISA ile incelendi. 735 adet abort yapmış koyunun 475 (%64.6) adedi ve 35 adet abort yapmış keçinin 2 (%5.7)’si pestivirus antikorları yönünden pozitif tespit edildi. Ayrıca, abort yapan koyunlardan toplanan lökosit örneklerinin 5 (%0.7)’inde ve bu hayvanlara ait atık fetus doku örneklerinde pestivirus antijen varlığı belirlenirken, abort yapmış keçilerin hiçbirinde antijen varlığı belirlenmedi. Sonuç olarak, Burdur bölgesinde abort yapan koyunlarda ve yavrularına ait doku örneklerinde spesifik antijen varlığı ve yüksek seroprevalans tespitinin, pestivirus enfeksiyonunun bu bölgede yaygUn ve koyunlarda gözlenen abort olgularında önemli bir nedeni olduğunu ortaya koymaktadır.

Anahtar Kelimeler:

Pestivirus, Burdur, koyun, akyuvarlar, keçi, koyunlar, hastalık yaygınlığı, yavru atma, ELISA

Burdur bölgesinde abort yapmış koyun ve keçilerde pestivirus enfeksiyonunun araştırılması

In this study, the presence of pestivirus reported as an important cause of abort cases in sheep and goats was investigated serologically and virologically in aborted sheep and goats. For this purpose, serum and leukocyte samples from 735 aborted sheep and 35 aborted goats, tissue samples (lung, spleen, brain) from 48 aborted or stillborn lambs were collected and these samples were tested by ELISA for the presence of pestivirus antigen and antibody. 475 (64.6%) of 735 aborted sheep and 2 (5.7%) of 35 aborted goats were seropositive for the presence of antibody to pestivirus. Also, the presence of pestivirus antigen was detected in only 5 (0.7%) of leukocyte samples collected from aborted sheep and in only 5 (10.4%) of tissue samples of aborted lambs belonging to them, whereas not detected in any of aborted goats. In conclusion, the results of this study that was done the detection of specific antigen and the detection of high seroprevalence in aborted sheep and aborted lambs belonging to them have brought up that infections of pestivirus are very widespread in Burdur province and that pestivirus is an important cause of abort cases in sheep in this province.

Keywords:

Pestivirus, Burdur, sheep, leukocytes, goats, ewes, disease prevalence, abortion, ELISA,

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