Mehmet NİYAZ, Hüseyin ÖZDEN, Gökhan KARACA, Çağatay E. DAPHAN, Huri BULUT, Muhammed GÖMEÇ

Topikal Mezenkimal Kök Hücre Uygulamasının İskemi Reperfüzyon Yaralanmasına Bağlı Kolonik Anastomoz Üzerine İyileştirici Etkileri

Bağırsak iskemi reperfüzyon hasarı (İRH) zorlu bir sorundur ve kolon anastomozunun iyileşmesini olumsuz etkiler. Deneysel çalışmamız kolon anastomozunun iyileşmesinde mezenkimal kök hücre (MKH) uygulamasının rolünü araştırmayı amaçlamıştır. Toplam 33 sıçan, Kontrol, İRH ve MKH tedavi grupları olarak gruplandı. MKH’leri elde etmek için üç sıçan ayrıldı. Tüm gruplara kolonik rezeksiyon ve anastomoz işlemi uygulandı. Anastomotik hat, MKH tedavi grubundaki sıçanlarda kolonik anastomozdan sonra MKH emdirilmiş spongostan ile sarıldı. Tüm sıçanlar ameliyat sonrası yedinci günde sakrifiye edildi ve inceleme için anastomoz hat örneklendi. Doku hidroksiprolin (HP) seviyeleri ve anastomoz patlama basınçları istatistiksel olarak karşılaştırıldı. MKH tedavi grubu sıçanlarda anastomoz patlama basınçları önemli ölçüde yüksek bulundu. En düşük anastomoz patlama basıncı İRH grubu sıçanlarda tespit edildi. Anastomotik bölgelerin hidroksiprolin içeriği, İRH grubu sıçanlara kıyasla MKH tedavi grubundaki sıçanlarda önemli ölçüde daha yüksek bulundu. Çalışmamız, deneysel bir modelde İRH’nin kolon anastomozunun iyileşme süreci üzerindeki zararlı etkilerinin MKH’lerin tedavisi ile hafifletilebileceğini göstermiştir.

The Healing Effects of The Topical Mesenchymal Stem Cells Application on Colonic Anastomosis Subjected to Ischemia Reperfusion Injury

Intestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis. Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic anastomosis. A total of 33 rats were grouped as Control, IRI and MSC treatment groups. Three rats were reserved for obtaining MSCs. Colonic resection and anastomosis procedure was performed in all groups. Anastomotic line was wrapped with MSCs impregnated spongostan after colonic anastomosis in the rats of the MSC treatment group. All rats were sacrificed and anastomotic line were sampled for examination on the post operative seventh day. Tissue hydroxyproline (HP) levels and anastomotic bursting pressures were statistically compared. Anastomotic bursting pressures were found to be significantly high in MSC treatment group rats. The lowest anastomotic bursting pressure was detected in IRI group rats. Hydroxyproline content of the anastomotic sites were also found to be significantly higher in the rats of the MSC treatment group when compared with the IRI group rats. Our study showed that the detrimental effects of IRI on the healing process of colonic anastomosis in an experimental model may be alleviated with the treatment of MSCs.

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