Hong JI, Jingru GUO, Chunyang NIU, Bin XU, Ziyi SHAO, Xu ZHANG, Xuelong ZHAN, Jianfa WANG, Ying LIU, Juxiong LIU

Farklı Derecelerde Soğuk Stresinin Domuzlarda FIAF Ekspresyonuna Etkileri

Soğuk stresi, soğuk bölgelerde hayvancılığın gelişmesini kısıtlayan ana stres faktörüdür. Anjiopoietin benzeri protein 4 (ANGPTL4) olarak da bilinenFasting-induced adipose faktör (FIAF), yağların metabolizmasında önemli bir düzenleyici rol oynar. FIAF’ın fonksiyonları arasında, kandaki trigliseritlerive serbest yağ asitlerini uzaklaştırmak için lipoprotein lipazı (LPL) inhibe etmek, yağ birikimini azaltmak ve yağ dokunun bozulmasını teşvik etmekbulunur. Bu çalışma, farklı derecelerde soğuk uyarımın, besili domuzlarda karaciğerin FIAF ve yağ metabolizması üzerine etkilerini araştırmak içintasarlanmıştır. Ergin ve besili domuzlar rastgele farklı gruplara ayrıldı ve 2 saat boyunca -10±2°C, -5±2°C, 0±2°C, 5±2°C ve 21±2°C sıcaklıklara maruzbırakıldı. Serum, karaciğer, boyun yağ dokusu, abdominal subkutanöz yağ doku ve mezenterik yağ doku toplandı ve FIAF ekspresyon analizi, kantitatifreal-time PCR (qRT-PCR), Western Blot ve Enzim-işaretli immünosorbent test (ELISA) ile gerçekleştirildi. Sonuçlar, soğuk stres yoğunluğundaki kademeliartışın, karaciğer, boyun yağ dokusu, karın yağ dokusu ve mezenterik yağ dokusunda FIAF’ın mRNA ve protein ekspresyon seviyelerinde kademeli birartışa neden olduğunu ve ayrıca kanda FIAF konsantrasyonunun da kademeli olarak arttığını gösterdi. FIAF’ın soğuk stresine yanıt olarak enerji ve yağmetabolizmasında rol oynadığını ve soğuk stresi ile indüklenen kandaki serbest yağ asitleri tarafından Peroksizom proliferatör-aktive reseptör (PPAR)aktivasyonu ile düzenlenebilir olduğunu gösterdi.

Eff ects of Diff erent Degrees of Cold Stress on FIAF Expression in Pigs

AbstractCold stress is the main stressor restricting the development of animal husbandry in cold regions. Fasting-induced adipose factor (FIAF), also known as angiopoietin-like protein 4 (ANGPTL4), plays an important regulatory role in the metabolism of lipids. Its functions include inhibiting lipoprotein lipase (LPL) to eliminate triglycerides and free fatty acids in blood, reducing fat deposition and promoting adipose tissue degradation. This experiment was designed to investigate the eff ects of diff erent degrees of cold stimulation on fat metabolism in finishing pigs. Growing and fattening pigs were randomly divided into diff erent groups and exposed to temperatures of -10±2°C, -5±2°C, 0±2°C, 5±2°C and 21±2°C for 2 h. Serum, liver, neck, abdominal subcutaneous and mesenteric adipose tissues were collected and analyzed by Real-Time quantitative PCR (qRT-PCR), western blotting and enzyme-linked immunosorbent assay (ELISA) to examine FIAF expression. The results showed that a gradual increase in cold stress intensity resulted in a gradual increase in FIAF mRNA and protein expression levels in liver, neck, abdomen and mesenteric adipose tissues and FIAF concentration also gradually increased in the blood. It indicated that FIAF is involved in energy and fat metabolism in response to cold stress and may be regulated by the activation of peroxisome proliferator-activated receptor (PPAR) by free fatty acids in the blood induced by cold stress.

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1. Scharf I, Wertheimer K, Xin JL, Gilad T, Goldenberg I, Subach A: Context-dependent effects of cold stress on behavioral, physiological, and life-history traits of the red flour beetle. Insect Sci, 26 (1): 142-153, 2019. DOI: 10.1111/1744-7917.12497
2. Cline DL, Short LI, Forster MAM, Gray SL: Adipose tissue expression of PACAP, VIP, and their receptors in response to cold stress. J Mol Neurosci, 68 (3): 427-438, 2019. DOI: 10.1007/s12031-018-1099-x
3. Luo L, Liu M: Adipose tissue in control of metabolism. J Endocrinol, 231 (3): R77-R99, 2016. DOI: 10.1530/JOE-16-0211
4. Harwood Jr HJ: The adipocyte as an endocrine organ in the regulation of metabolic homeostasis. Neuropharmacology, 63 (1): 57-75, 2012. DOI: 10.1016/j.neuropharm.2011.12.010
5. Yang J, Li X, Xu D: Research progress on the involvement of ANGPTL4 and loss-of-function variants in lipid metabolism and coronary heart disease: Is the „prime time“ of ANGPTL4-targeted therapy for coronary heart disease approaching? Cardiovasc Drugs Ther, 2020. DOI: 10.1007/ s10557-020-07001-0
6. Kersten S, Lichtenstein L, Steenbergen E, Mudde K, Hendriks HFJ, Hesselink MK, Schrauwen P, Müller M: Caloric restriction and exercise increase plasma ANGPTL4 levels in humans via elevated free fatty acids. Arterioscler Thromb Vasc Biol, 29 (6): 969-974, 2009. DOI: 10.1161/ ATVBAHA.108.182147
7. Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods, 25 (4): 402-408, 2001. DOI: 10.1006/meth.2001.1262
8. Li SZ, Yang YY, Yang HM, Ni J: Study on comparative-physiology of some endocrinal activities on cold exposure and cold acclimation in rats and chicks. Chin J Appl Physiol, 24 (1): 23, 2008. DOI: 10.13459/j.cnki. cjap.2008.01.002
9. Fu SP, Ren BY, Liu Y, Li SN, Li Y, Wang MP, Yang ZQ, Wei LB, Li ZQ, Liu JX, Wang W, Guo WB, Hu ZM: Adiponectin and its receptors mRNA levels in swine adipose tissue in the condition of cold stress. Chin Anim Husb Vet Med, 39 (3): 28-32, 2012.
10. Kersten S, Mandard S, Tan NS, Escher P, Metzger D, Chambon P, Gonzalez FJ, Desvergne B, Wahli W: Characterization of the fastinginduced adipose factor FIAF, a novel peroxisome proliferator-activated receptor target gene. J Biol Chem, 275 (37): 28488-28493, 2000. DOI: 10.1074/jbc.M004029200
11. Dijk W, Kersten S: Regulation of lipid metabolism by angiopoietinlike proteins. Curr Opin Lipidol, 27 (3): 249-256, 2016. DOI: 10.1097/ MOL.0000000000000290
12. Desai U, Lee EC, Chung K, Gao CH, Gay J, Key B, Hansen G, Machajewski D, Platt KA, Sands AT, Schneider M, Sligtenhorst IV, Suwanichkul A, Vogel P, Wilganowski N, Wingert J, Zambrowicz BP, Landes G, Powell DR: Lipid-lowering effects of anti-angiopoietin-like 4 antibody recapitulate the lipid phenotype found in angiopoietin-like 4 knockout mice. Proc Natl Acad Sci U S A, 104 (28): 11766-11771, 2007. DOI: 10.1073/pnas.0705041104
13. Zhang LP: Study on the relationship of gut microbiota with body fat and ANGPTL4 gene in different breeds of pigs. Sichuan Agricultural University, 2007.
14. Gettys TW, Harkness PJ, Watson PM: The beta 3-adrenergic receptor inhibits insulin-stimulated leptin secretion from isolated rat adipocytes. Endocrinology, 137 (9): 4054-4057, 1996. DOI: 10.1210/endo.137.9.8756584
15. Delporte ML, Funahashi T, Takahashi M, Matsuzawa Y, Brichard SM: Pre- and post-translational negative effect of beta-adrenoceptor agonists on adiponectin secretion: In vitro and in vivo studies. Biochem J, 367 (3): 677-685, 2002. DOI: 10.1042/BJ20020610
16. Kvetnansky R, Ukropec J, Laukova M, Manz B, Pacak K, Vargovic P: Stress stimulates production of catecholamines in rat adipocytes. Cell Mol Neurobiol, 32 (5): 801-813, 2012. DOI: 10.1007/s10571-012-9822-6
17. Liu Y, Ren WZ, Li SN, Li WH, Fu SP, Wu YK, Wang K, Hu ZM: PPARγ2 gene expression of swine adipose tissue liver heart and kidney in cold stress. Chin J Vet Sci, 32 (4): 592-597, 2012. DOI: 10.16303/j.cnki.1005- 4545.2012.04.022