İsmail COŞKUNER, Meltem TÜRKAY, HÜSEYİN YILDIZ, EMİN SİLAY, Hamide SAYAR, Zeynep BAYAT

The Protective Effect of Thiopental Againts Renal Ischemia/ Reperfusion Damage in Rats

Çalışmada ratlarda farklı dozlarda uygulanan tiyopentalin renal I/R hasarına karşı koruyucu optimal dozunun saptanması amaçlandı. Bu çalışma 42 ratta yapıldı. Abdominal orta hat insizyonu sonrası sol renal arter okluzyonu yapıldı. Altmış dakika işemi ve reperfüzyondan sonra sol nefrektomi yapıldı. Altışar rattan oluşan gruplarda (sham grup dışında) cerrahi prosedür uygulandı. Kontrol grubunda sol böbreklere 60 dk işemi ve reperfüzyon uygulandı. Sham ve kontrol grubu dışındaki ratlara reperfüzyondan 15 dk önce tiyopental (3, 10, 30, 60, 80 mg/kg) intra-peritoneal olarak uygulandı. Reperfüzyon sonunda IL-1?, IL-6, TNF-? seviyeleri; SOD aktivitesi; MDA ve NO düzeyleri ölçüldü. Gruplarda IL-6 ve TNF-? seviyelerinde azalma olmadı. Yalnızca 3 mg/kg tiyopental uygulanan grupta IL-1? seviyesi azaldı. MDA düzeylerinde düşme yalnızca 80 mg/kg tiyopental uygulanan grupta görüldü. 30 mg/kg tiyopental uygulanan ratlarda SOD düzeylerinde anlamlı yükseklik saptandı. 3 mg/kg tiyopental uygulanan ratlarda glutatyon peroksidaz (GPx) düzeylerinde anlamlı yükseklik saptandı. Nitrik oksit seviyesindeki düşme 10, 30 ve 60 mg/kg tiyopental uygulanan gruplarda görüldü. Çalışma sonuçlarına göre, renal işemi reperfüzyon hasarında her bir biyokimyasal belirteci tiyopentalin farklı dozları etkiledi.

Ratlarda Böbrek İşemi/Reperfüzyon Hasarına Karşı Tiyopentalin Koruyucu Etkisi

The aim of this study was to determine optimal protective thiopental dose in renal ischemia/reperfusion (I/R) damage. This study was carried out on 42 rats. After abdominal midline incision, left renal artery was occluded. After 60 min of ischemia and reperfusion, a left nephrectomy was performed. Surgical intervention was made for the six rat groups (excluding sham group). In control group, 60 minutes of ischemia and reperfusion were applied to left kidneys. Thiopental (3, 10, 30, 60, 80 mg/kg) was applied intra-peritoneally to the rats except sham and control groups 15 minutes before reperfusion. After reperfusion, IL-1?, IL-6, TNF-? levels; SOD activity; MDA and NO levels were measured. There was no decrease in IL-6 or TNF-? levels in groups at all doses. IL-1? levels only decreased in the group receiving 3 mg/kg thiopental. Only the group receiving 80 mg/kg observed decrease in MDA levels. The rats receiving 30 mg/kg showed significant increase in SOD levels. The rats receiving 3 mg/kg detected significant increase in glutathione peroxidase (GPx) levels. The groups receiving 10, 30, and 60 mg/kg thiopental showed decrease in nitric oxide (NO) levels. According to study results, different doses of thiopental affected each biochemical indicator in renal I/R damage.

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