Yaşar BOZKURT, Uğur FIRAT, Murat ATAR, Ahmet Ali SANCAKTUTAR, Necmettin PEMBEGUL, Haluk SÖYLEMEZ, Hatice YÜKSEL, Harun ALP, Mehmet Nuri BODAKÇI, Namık Kemal HATİPOĞLU, Sadık BÜYÜKBAŞ

The protective effect of ellagic acid against renal ischemia-reperfusion injury in male rats

Bu çalışmanın amacı ratlarda renal iskemi-reperfüzyon (I/R) hasarına karşı elajik asitin (EA) olası koruyucu etkisini değerlendirmektir. Yirmi dört erkek Wistar rat; Sham, I/R ve I/R+EA olmak üzere üç gruba ayrıldı. Sham grubuna laparotomi yapıldı ve iskemi uygulanmaksızın 45 dakika bekletildi. I/R grubuna 45 dakikalık sol renal iskemiyi takiben 60 dakikalık reperfüzyon uygulandı. I/R+EA grubuna da I/R grubundakilere benzer şekilde iskemi/reperfüzyon prosedürü uygulandı, fakat iskemiden 30 dakika önce ratlara 85 mg/kg EA ağızdan verildi. Kanda ve böbrek dokusunda malondialdehit (MDA), total antioksidan kapasite (TAC), total oksidan statusu (TOS) ve oksidatif stres indeksi (OSI) bakıldı. Böbrek dokusu histopatolojik yönden incelendi. Sham grubu ile kıyaslandığında I/R grubunda serum MDA düzeyi anlamlı derecede yüksek idi. Ayrıca I/R+EA grubu ile kıyaslandığında I/R grubunda serum TAC düzeyi anlamlı derecede düşük bulundu. I/R grubunda böbrek dokusunda OSI düzeyi yüksek TAC düzeyi düşük bulunurken, I/R+EA grubunda iskemi ile oluşan etkilerin EA ile düzeldiği görüldü. EA verilmesinin tubuler nekroz skorunu azaltmada etkili olduğu bulundu. Çalışmanın sonucuna göre; EA, renal I/R hasarı sonrası meydana gelen oksidatif hasarı ve oluşan histopatolojik değişiklikleri iyileştirmektedir.

Anahtar Kelimeler:

hayvan modelleri, hastalık modelleri, ellagik asit, histopatoloji, iskemi, böbrek hastalıkları, sıçan, travma

Erkek ratlarda renal iskemi-reperfüzyon hasarına karşı elajik asitin koruyucu etkisi

The aim of this study was to evaluate the possible protective effect of ellagic acid (EA) on rats following renal ischemia–reperfusion (I/R) injury. Twenty-four Wistar rats were divided into three groups. Sham group underwent laparotomy then waited for 45 min without ischemia. I/R group were subjected to left renal ischemia for 45 minutes followed by 60 min of reperfusion. I/R+EA group were subjected to the same renal ischemia/reperfusion as the I/R group, were also given 85 mg/kg EA perorally 30 min prior to the ischemia. Malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined on the blood samples and kidney tissues. Histopathological analyses were conducted on the kidney tissues. I/R damage significantly increased serum MDA levels in the I/R group when compared with Sham group. Serum TAC level was significantly lower in I/R group than I/R+EA group. A significantly increase on OSI levels and decrease on TAC levels was found in the kidneys in I/R group. In I/R + EA group, EA reversed the negative effects of I/R injury. EA pretreatment was effective in decreasing tubular necrosis score. In conclusion; EA pretreatment ameliorated the oxidative damage and histopathological changes occurring following renal I/R injury.

Keywords:

animal models, disease models, ellagic acid, histopathology, ischaemia, kidney diseases, rats, trauma,

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