Faruk PEHLIVANOGLU, HAKAN YARDIMCI

Detection of methicillin and vancomycin resistance in staphylococcus strains ısolated from bovine milk samples with mastitis]

Bu çalışmada, klinik ve subklinik mastitisli inek süt örneklerinden izole edilen stafilokok suşlarında metisilin ve vankomisin dirençliliğinin belirlenmesi amaçlanmıştır. Burdur ilindeki süt ineği çiftliklerinden toplanan 306 meme lobundan toplanan süt örneklerinden 100 stafilokok suşu izole edildi. Metisilin ve vankomisine fenotipik dirençlilik Agar-Disk Difüzyon Tekniği (ADDT) ile ve metisilin ve vankomisin için Minimal İnhibitör Konsantrasyonu (MİK) değerleri Agar Dilüsyon Tekniği (ADT) ile Klinik ve Laboratuvar Standartları Enstitüsü (CLSI) protokollerine göre belirlendi. Suşlarda mecA ve vanA genlerinin belirlenmesi için Polimeraz Zincir Reaksiyonu (PZR) gerçekleştirildi. Hem fenotipik hem de genotipik olarak metisiline dirençli ve duyarlı olan suşların sayısı sırasıyla 20 ve 25 olarak bulundu. On adet stafilokok suşu vankomisine duyarlılığı azalmış olarak belirlendi. Suşların hiç biri vanA genine sahip değil idi. Sonuç olarak, Burdur ilinde sığır mastitislerinden sorumlu stafilokok suşlarında metisilin dirençliliği yüksektir. Vankomisin dirençliliği açısından ise, dirençli suş bulunmamakta fakat vankomisine duyarlılığı azalmış suşların varlığı dikkate alınmalıdır.

Anahtar Kelimeler:

Staphylococcus, sığır meme yangısı, saptama, genler, meme yangısı, metisilin, süt, çoklu ilaç direnci, polimeraz zincir reaksiyonu, soy, vankomisin

Mastitisli sığır sütlerinden izole edilen stafilokok suşlarında metisilin ve vankomisin dirençliliğinin belirlenmesi

In this study, determination of resistance to methicilline and vancomycin in staphylococcus strains isolated from cow milk samples with subclinical and clinical mastitis was aimed. One hundred staphylococcus strains were isolated from total 306 quarter milk samples collected from the dairy cattle farms in the city of Burdur. Phenotypic resistance to methicillin and vancomycin was determined by Agar-Disc Diffusion Technique (ADDT) and Minimal Inhibitory Concentration (MIC) values for methicillin and vancomycin were determined by Agar Dilution Technique (ADT), according to Clinical and Laboratory Standards Institute (CLSI) protocols. Polymerase Chain Reaction (PCR) was performed for determination of mecA and vanA genes in the strains. The number of strains with both phenotypically and genotypically resistant and susceptible to methicillin were found as 20 and 25, respectively. Ten staphylococcus strains with reduced susceptibility to vancomycin were determined. None of the strains had vanA gene. In conclusion, it is stated that methicillin resistance in staphylococcus strains responsible from mastitis in cattle in Burdur province is high. In terms of vancomycin resistance there is no resistant staphylococcus strains, but presence of strains with reduced susceptibility to vancomycin should be taken into account.

Keywords:

Staphylococcus, bovine mastitis, detection, genes, mastitis, methicillin, milk, multiple drug resistance, polymerase chain reaction, strains, vancomycin,

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