Identification of nonstarter ındigenous lactobacilli from kars gravier cheese
Bu çalışmanın amacı, Kars Gravyer peynirinin hakim mikroflarasını oluşturan doğal laktobasillerin tanımlanmasıdır. Araştırmada, Kars Gravyer peynirinden 120 adet laktobasil suşu izole edilmiş ve API 50 CHL sistemi ile tanımlanmıştır. Tanımlanan suşların sayı ve oranları aşağıdaki gibidir; 30 tanesi (%25) L. brevis tip 1, 22 tanesi (%18.3) Pediococcus pentosaceus tip 1, 17 tanesi (%14.1) Lactobacillus paracasei ssp paracasei, 16 tanesi (%13.3) Lactobacillus fermentum tip 1, 15 tanesi (%12.5) Lactococcus lactis ssp lactis tip 1, 9 tanesi (%7.5) Lactobacillus delbrueckii ssp lactis tip 1, 6 tanesi (%5) Lactobacillus plantarum tip 1 ve 5 tanesi (%4.16) Lactobacillus rhamnosus olarak tanımlanmıştır. Araştırmada elde edilen bulgular ışığında, Kars Gravyer peynirindeki dominant mikroflorayı Lactobacillus türlerinin oluşturduğu söylenebilir.
Kars gravyer peynirinden izole edilen doğal laktobasillerin tanımlanması
The aim of this study was to identify indigenous nonstarter lactobacilli (NSLAB) isolated from Kars Gravier cheese. In this study, 120 isolates of NSLAB from Kars Gravier cheese were isolated and identified with the API 50 CHL system. The ratio of identified NSLAB strains were as follows; 30 (25%) were Lactobacillus brevis type 1, 22 (18.3%) were Pediococcus pentosaceus type 1, 17 (14.1%) were Lactobacillus paracasei ssp. paracasei, 16 (13.3%) were Lactobacillus fermentum type 1, 15 (12.5%) were Lactococcus lactis ssp. lactis type 1, 9 (7.5%) were Lactobacillus delbrueckii ssp. lactis type 1, 6 (5%) were Lactobacillus plantarum type 1 and 5 (4.16%) of them were Lactobacillus rhamnosus. In the light of the results obtained in this study, it could be said that Lactobacillus species formed the dominant microflora of Kars Gravier cheese.
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- 1. Madiedo PR, Hugenholtz J, Zoon P: An overview of the functionality of exopolysaccharides produced by lactic acid bacteria. Int Dairy J, 12, 163-171, 2002.
- 2. Coeuret C, Dubernet MB, Gueguen M, Vernoux JP: Isolation, characterisation and identification of lactobacilli focusing mainly on cheeses and other dairy products. Lait, 83, 269-306, 2003.
- 3. Silvester ME, Dicks LMT: Identification of lactic acid bacteria isolated from human vaginal secretions. Anton van Leeuw, 83, 117-123, 2003.
- 4. Kılıç S: Süt Endüstrisinde Laktik Asit Bakterileri. I. Baskı, Ege Üniv. Ziraat Fak. Yayınları No: 542, Bornova- İzmir, 2001.
- 5. Laan H, Tan SE, Bruinenberg P, Limsowtin G, Broome M: Aminopeptidase activities of starter and non-starter lactic acid bacteria under simulated Cheddar Cheese ripening conditions. Int Dairy J, 8 (4): 267-274,1998.
- 6. Mannu L, Comunian R, Scintu MF: Mesophilic lactobacilli in Fiore Sardo cheese: PCR-identification and evolution during cheese ripening. Int Dairy J, 10, 383-389, 2000.
- 7. Banks JM, Williams AG: The role of the nonstarter lactic acid bacteria in Cheddar cheese ripening. Dairy Tech, 57 (2/3): 145-152, 2004.
- 8. Williams AG, Withers SE, Banks JM: Energy sources of non-starter lactic acid bacteria isolated from Cheddar Cheese. Int Dairy J, 10 (1-2): 17-23, 2000.
- 9. Angelis MD, Corsetti A, Tosti N, Rossi J, Corbo MR, Gobbetti M: Characterization of non-starter lactic acid bacteria from ıtalian ewe cheeses based on phenotypic, genotypic, and cell wall protein analyses. Appl Environ Microbiol, 67 (5): 2011-2020, 2001.
- 10. Hayaloglu AA, Fox PF, Guven M, Cakmakcı S: Cheeses of Turkey: 1. Varieties ripened in goat-skin bags. Lait, 87, 79-95, 2007.
- 11. Turgut T, Erdoğan A, Atasever M: Karın Kaymağı peynirinden izole edilen laktobasillerin tanımlanması. Kafkas Univ Vet Fak Derg, 18 (2): 209-213, 2012.
- 12. Kamber U, Elmalı M, Yaman H: Microbiological and chemical properties of Gravier cheeses. Atatürk Üniv Vet Bil Derg, 3 (1): 1-7, 2008.
- 13. Çetinkaya A, Yaman H, Elmalı M, Karadağoğlu G: Geleneksel Lezzetler - Kars Gravyer peyniri. Gıda Mühendisliği Derg, 22 (10): 39-40, 2006.
- 14. Ulutaş Z, Çağlar A, Kurt A: Kars Gravyer peynirinin yapılışı, duyusal, fiziksel ve kimyasal özellikleri üzerine bir araştırma. Gıda, 18 (3): 197-202, 1993.
- 15. Anonymous: Gravyer peyniri. TS 2174. Türk Standartları Enstitüsü. 100. Yıl Bulvarı, No: 99, Kat: 3, Ostim/Ankara, 1989.
- 16. Tynkkynen S, Satokari R, Saarela M, Sandholm TM, Saxelin M: Comparison of ribotyping, randomly amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis in typing of Lactobacillus rhamnosus and L. casei strains. Appl Environ Microbiol, 66 (9): 3908-3914, 1999.
- 17. Harrigan WF: Laboratory Methods in Food Microbiology, 3rd ed,, 532 p., Academic Press, London, 1998.
- 18. Özgün D, Vural Cingilli H: Identification of Lactobacillus strains isolated from faecal specimens of babies and human milk colostrum by API 50 CHL system. J Med Genet Genom, 3 (3): 46-49, 2011.
- 19. Giraffa G, DeVecchi P, Rossetti L: Note: Identification of Lactobacillus delbrueckii subspecies bulgaricus and subspecies lactis dairy isolates by amplified rDNA restriction Analysis. J Appl Microbiol, 85, 918-924, 1998.
- 20. Öner Z, O Sağdıç, Şimşek B: Lactic acid bacteria profiles and tyramine and tryptamine contents of Turkish tulum cheeses. Eur Food Res Technol, 219 (5): 455-459, 2004.
- 21. Şengül M, Çakmakçı S: Characterization of natural isolates of lactic acid bacteria from Erzincan (Savak) Tulum cheese. Milchwissenschaft, 58 (9): 510-513, 2003.
- 22. Sağdıç O, Şimşek B, Küçüköner E: Microbiological and physico-chemical characteristics of Van herby cheese, a traditional Turkish dairy product. Milchwissenschaft, 58 (7-8): 382-385, 2003.
- 23. Öksüztepe G, Patır B, Çalıcıoğlu M: Identification and distribution of lactic acid bacteria during the ripening of Şavak tulum cheese. Turk J Vet Anim Sci, 29, 873-879, 2005.