Muhammad Abdul Rauf MALIK, Muhammad IJAZ, Amjad Islam AQIB, Shahid Hussain FAROOQI, Kashif HUSSAIN

The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk

Bu çalışma; zoonotik bir patojen olan koagülaz pozitif Staphylococcus aureus'un prevalansını ve bu patojenin yayılmasında rol oynayan risk fakörlerini tanımlamaktadır. Faisalabad'ın 3 ayrı bölgesindeki hayvanlardan toplanan toplam 900 adet süt örneğinde (n = 450 sığır ve n = 450 manda) Staphylococci identifikasyonu amacıyla mikrobiyolojik analiz ve coa geninin tespiti amacıyla PCR gerçekleştirildi. Subklinik mastitisin taranmasında sağım esnasında sörf mastitis testi uygulandı. Risk faktörlerinin mastitisle ilişkisini değerlendirmek amacıyla Ki-kare testi kullanıldı. Sığır ve manda sütlerinde subklinik mastitis prevalansı sırasıyla %54 ve %56'sı olarak hesaplanırken toplamda %55 olarak bulundu. Farklı şehirlerden örneklenen sığırlar arasında subklinik mastitis prevalansı yönünden anlamlı bir fark tespit edildi (P

İnek Sütünde Koagulaz Pozitif Staphylococcus aureus Coa Geni Prevalansı ve İlgili Risk Faktörleri

The study describes prevalence of coagulase positive Staphylococcus aureus, a zoonotic pathogen, and rule out key risk factors associated with the spread of this pathogen. A total of 900 milk samples of animals (n = 450 cattle and n = 450 buffaloes) from three subdistricts of Faisalabad were collected and processed microbiologically for staphylococci identification while, PCR for coa gene identification. Surf field mastitis test was used to screen the samples for subclinical mastitis at milking. Chi-square test was used to assess association of risk factors with mastitis. The overall prevalence of subclinical mastitis was 55% comprising 54 and 56% in cattle and buffalo’s milk, respectively. A significant difference (P

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