Ling YANG, Baoliang LIU, Xianxi YAN, Leying ZHANG, Feng GAO, Zhichaorn LIU

Expression of ISG15 in Bone Marrow During Early Pregnancy in Ewes

İnterferon-tau (IFNT) ruminantlarda gebeliğin maternal tanısındaki başlıca sinyaldir. IFNT endometrium, korpus luteum ve karaciğer üzerinde parakrin ve endokrin yollarla etki ederek luteolitik mekanizmanın gelişmesini inhibe eder ve semi-allojenik fetusun anne tarafından reddini baskılar. Kemik iliği lenfatik sistemin en önemli bileşenlerinden biri olup lenfositleri üretmek suretiyle vücudun bağışıklık sistemini desteklemektedir. Bu çalışmada, 13, 16 ve 25. günlerde gebe koyunlardan ve 16. günde gebe olmayan koyunlardan kemik iliği alınarak interferon tarafından uyarılan gen 15 kDa protein (ISG15) mRNA ve protein ekspresyonu qRT-PCR, Western blot ve immunohistokimyasal yöntemlerle araştırıldı. Elde edilen sonuçlar ISG15 mRNA, proteinler ile konjuge proteinlerin ekspresyonunun erken gebelik döneminde kemik iliğinde upregule edildiğini ve immunohistokimyasal olarak ISG15 proteinlerin kemik iliğinin stromasında farklı hücrelerin sitoplazmasında yer aldığını göstermiştir. Sonuç olarak, gebeliğe bağlı olarak üretilen IFNT; ISG15 ve konjuge proteinlerin ekspresyonunu kemik iliğinin stromasında endokrin yolla upregüle etmiş ve böylece koyunların erken gebelik döneminde maternal bağışıklık cevabın düzenlenmesinde rol almıştır

Koyunların Erken Gebelik Döneminde Kemik İliğinde ISG15 Ekspresyonu

Interferon-tau (IFNT) is the main signal for maternal recognition of pregnancy in ruminants. IFNT acts on the endometrium, corpus luteum, and liver through paracrine and endocrine style, which is involved in inhibiting the development of luteolytic mechanism and suppressing maternal immune rejection of the semi-allogeneic fetus. Bone marrow (BM) is a key component of the lymphatic system, supports the body’s immune system through producing the lymphocytes. At present study, the BM was obtained from days 13, 16 and 25 of pregnant ewes, day 16 of non-pregnant ewes to study the expression of interferon stimulated gene 15 kDa protein (ISG15) mRNA and protein through a qRT-PCR assay, Western blot, and an immunohistochemistry analysis. Our results showed that the expression of ISG15 mRNA, proteins and conjugated proteins were up-regulated in the stroma of BM during early pregnancy, and the immunohistochemistry results confirmed that the ISG15 proteins were localized in the cytoplasm of different cells in the stroma of BM. In conclusion, IFNT derived from the conceptus induced upregulated expression of ISG15 and conjugated proteins in the stroma of BM through an endocrine style, which were involved in regulating the maternal immune response during early pregnancy in ewes

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