MURAT SARIERLER, Okan BÖLÜKBAŞI, ERKUT TURAN, BÜLENT ULUTAŞ

The effects of different anaesthetic agents on electrodiagnostic parameters in Rats

Bu çalışmanın amacı, ratlarda göğüs ve solunum kasları olan rectus abdominis, diyafram ve interkostal kasların elektrodiyagnostik parametreleri üzerine farklı anestezik maddelerin olası etkilerini belirlemektir. Bu amaçla 10 adet yetişkin, erkek Sprague-Dawley rat, eter ile anesteziye alındı, konsantrik iğne elektrodlar ve yüzeysel uyarıcılar kullanılarak, rectus abdominis, interkostal ve diyafram kaslarının elektromyografik (EMG) aktiviteleri ile birlikte n. phrenicus ve n. ischiadicus’un latansları kaydedildi. Aynı uygulama, birer hafta arayla ksilazin/ketamin ve fentanil/ketamin kombinasyonları ile tekrarlandı. Aynı hayvanlar üzerinde farklı anesteziklerle ilgili elektrodiyagnostik bulgular karşılaştırıldı ve gruplar arasında farklılıkların olduğu görüldü. Sinir iletim çalışmaları, eter ve ksilazin/ketamin anestezilerinin n. ischiadicus’un motor sinir aksiyon potansiyeli latansında, istatistiksel olarak önemli (P

Anahtar Kelimeler:

anestetikler, teşhis, tanı teknikleri, sıçan

Ratlarda, farklı anesteziklerin elektrodiyagnostik parametrelere etkisi

The purpose of this study was to determine the possible effects of different anaesthetic agents on electrodiagnostic parameters in rectus abdominis, diaphragm and intercostal muscles which represent the muscles of trunk and respiratory, respectively in rat. For this purpose, ten adult, male, Sprague-Dawley rats were anaesthetized with ether, and rectus abdominis, intercostal and diaphragm muscle electromyographic (EMG) activities also with latency of n. phrenicus and n. ischiadicus were recorded in sequence by using concentric needle electrodes and surface stimulator. The same procedure was applied with xylazine/ketamine and fentanyl citrate/ketamine combinations at one week interval. Results of electrodiagnostic findings related to different anaesthetics in the same animal were compared and it was seen that parameters were different among the groups. Nerve conduction studies showed that ether and xylazine/ketamine caused significantly (P<0.01) prolongation of motor nerve action potential latency (MNAPL) of nervus ischiadicus. Although the prolongation of MNAPL of n. phrenicus was observed in xylazine/ketamine group, it was not significantly different between groups. There was no significant difference on the EMG activity of rectus abdominis, intercostal and diaphragm muscles following the administration of ether, xylazine/ketamine and fentanyl/ketamine. This study showed some evidence about the effects of different anaesthetics on some electrodiagnostic parameters in rats. In electrophysiological studies using anaesthetics to keep the animals immobile, it will be kept in mind that the different anaesthetics may have influence on the nerve conduction latency.

Keywords:

anaesthetics, diagnosis, diagnostic techniques, rats,

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1.White PF, Way WL, Trevor AJ: Ketamine-Its pharmacology and therapeutic uses. Anesthesiology, 56, 119-136, 1982.
2.Winters WD, Ferrar-Allado T, Guzman-Flores C, Alcaraz M: The cataleptic state induced by ketamine: A review of the neuropharmacology of anaesthesia. Neuropharmacol, 11, 303-315, 1972.
3.Flecknell P: Laboratory Animal Anaesthesia. Third edition. Academic Press San Diego, USA, 2009
4.Waynforth HB, Flecknell PA: Experimental and Surgical Technique in the Rat. Academic Press, San Diego, USA, 1994.
5.Wixson SK, White WJ, Hughes Jr HC, Marshall WK, Lang CM: The effects of pentobarbital, fentanyl-droperidol, ketamine-xylazine and ketamine-diazepam on noxious stimulus perception in adult male rats. Lab Anim Sci, 37 (6): 731, 1987.
6.Bailey PL, Wilbrink J, Zwanikken P, Pace NL, Stanley TH: Anaesthetic induction with fentanyl. Anesth Analg, 64, 48-53, 1985.
7.Lui PW, Lee TY, Chan SHH: Fentanyl induced muscle rigidity in unanesthetized and ketamine- or thiopental anesthetized rats. Anesthesiology, 70, 984-990, 1989.
8.Stanley TH, Philbin DM, Coggins CH: Fentanyl-oxygen anaesthesia for coronary artery surgery: Cardiovascular and antidiuretic hormone responses. Can Anaesth Soc J, 26, 168172, 1979.
9.Comstock MK, Carter JG, Moyers JR, Stevens WC: Rigidity and hypercarbia associated with high dose fentanyl induction of anesthesia. Anesth Analg, 60, 362, 1981.
10.Freud FG, Martin EW, Wong KC, Hornbein TF: Abdominal muscle rigidity induced by morphine and nitrous oxide. Anesthesiology, 38, 358-362, 1973.
11.Lai S, Lui P: Inhibition by neuropeptide Y of fentanyl-induced muscular rigidity at the locus coeruleus in rats. Neurosci Lett, 280 (3): 203-6, 2000
12.Scammon FL: Fentanyl-O2 –N2O rigidity and pulmonary compliance. Anesth Analg, 62, 332-334, 1983.
13.Benthuysen JL, Kien ND, Quam DD: Intracranial pressure increases during alfentanil-induced rigidity. Anesthesiology, 68, 438-440, 1988.
14.Benthuysen JL, Smith NT, Sanford TJ, Head N, Dec-Silver H: Physiology of alfentanil induced rigidity. Anesthesiology, 64, 440-446, 1986.
15. Janczewski WA, Onimaru H, Homma I, Feldman JL: Opioid-resistant respiratory pathway from the preinspiratory neurones to abdominal muscles: In vivo and in vitro study in the newborn rat. J Physiol, 545 (3): 1017-26, 2002.
16.Campbell C, Weinger MB, Quinn M: Alterations in diaphragm EMG activity during opiate-induced respiratory depression. Respir Physiol, 100, 107-117, 1995.
17.Tsou M, Lui PW, Lee TY, Pan JT, Chan S: Differential effects of prazosin and yohimbine on fentanyl-induced muscular rigidity in rats. Neuropharmacol, 28 (11): 1163-1168, 1989.
18.Horrow J, Abrams J, Van Ripper D, Lambson D, Storella R: Ventilatory compliance after three sufentanil-pancuranium induction sequences. Anesthesiology, 75, 969-974, 1991.