The effect of carbon tetrachloride $(CCl_4)$ AND ETHANOL $(C_2H_5OH)$ on the determination of levels glutathione peroxidase, catalase, glucose-6-phosphate dehidrogenase and lipid peroxidation liver and kidney in the goose

Plazma ve dokularda lipid peroksid düzeyleri çeşitli organ ve hücrelerdeki bozunmaya bağlı hastalıklar ile pozitif bir korelasyon gösterir. Çalışmada Kaz karaciğeri ve böbreklerinde karbon tetraklorür ve etil alkol ile oluşturulan doku hasarlarında lipid peroksidasyonunun bir ürünü olan malondialdehit ve antioksidan defans sistemi araştırıldı. Çalışmada 40 adet 200-250 g klinik olarak sağlıklı 3 haftalık Anser anser ırkı kaz palazı kullanıldı. Hayvanlar 4 eşit Gruba ayrıldı. Birinci grup kontrol grubunu oluşturmuştur. Bu Grup standart yem ve su ile ad libutum olarak beslendi. İkinci Gruba canlı ağırlık başına 1 ml/kg CC14, III. Gruba 1.5 ml/kg CC14 ve IV. gruba da %50'lik etil alkolden kilogram başına 5 ml/kg haftada üç kez olmak üzere 12 hafta boyunca oral yolla verildi. Bu çalışma sonunda karaciğer ve böbreklerde hasara bağlı olarak malondialdehide (MDA) düzeylerinde artış, glutatyon peroksidaz" (GSH-Px), katalaz (CAT), glukoz-6fosfat dehidrogenaz (G6PD) aktivitelerinde azalma görüldü.

Anahtar Kelimeler:

lipit peroksidasyonu, glutatyon peroksidaz, glukoz-6-fosfat dehidrogenaz, etanol, katalaz, karbon tetraklorür, kaz, toksisite, toksik maddeler, kümes hayvanları, böbrekler, karaciğer

Karbon tetraklorür $(CCl_4)$ ve etil alkol $(C_2H_5OH)$ ün kaz karaciğer ve böbreklerinde glutatyon peroksidaz, katalaz, glukoz-6-fosfat dehidrogenaz enzim aktiviteleri ve lipid peroksidaz düzeylerine etkilerinin belirlenmesi

The elevation of plasma and tissue lipid peroxide levels is an indicator of membrane disruption in various tissue and organ cells and it is positively correlated with the gravity of the disease. Changes in the liver of malondialdehyde (MDA, a degreedative product of lipid peroxidation) and in the antioxidant defence system in geese with alcohol and carbon tetrachloride-induced liver and kidney degeneration were studied. The experiments were carried out on 3 week-old clinically healthy (40 animals), geese (Anser anser) weighing 200-250 g divided into control and experimental groups of 10 animals each. The first group was used as the control group. The second group was given 1 ml/kg b.w. CC14 while the third group was given 1.5 ml/kg b.w. CC14. The fourth group was given 50 % ethanol (5 ml/kg b.w). The treatment was made tree times a week for 12 weeks. 1 and 1.5 (ml/kg b.w) doses of CC14 and ethanol increased the malondialdehyde level of the liver as a consequence of the lipid peroxidation. At the results of this study malondialdehide (MDA) levels was increase and glutathione peroxidase (GSH-Px), catalase (CAT), glucose-6 phosphate dehidrogenase (G6PD) were decrease as according to the liver and kidney tissue's degeneration

Keywords:

lipid peroxidation, glutathione peroxidase, glucose-6-phosphate dehydrogenase, ethanol, catalase, carbon tetrachloride, geese, toxicity, toxic substances, poultry, kidneys, liver,

PDF

___

1 Mason RP: Free-radical intermediates in the metabolism of toxic chemicals; Free Radicals in Biology, 5,161-222, 1982.
2 Yılmaz S, Bahçelioğlu HI: Kârbontetraklorür ile siroz oluşturan ratlarda lipid peroksidasyonu. Antioksidant enzim ile pirüvat kinaz akti viteleri. Tr J Vet Anim Sci, 24,25-28,2000.
3 Bey tut E, Güven A, Kamiloğlu NN: Alterations in erthrocyte antioxidant system after chronic administration of high dose of ethanol to mice.Kafkas ÜnivVet Fak Derg, 9,137-140,2003.
4 Güven A, Maraşlı N, Kaya N: Changes in the lipid peroxide status of geese in chronic carbon tetrachloride poisoning, Indian VetJ, 80:508-510; 2003.
5 DiLuzio NR: Antioxidants; lipid peroxidation and chemical- induced liverinjüry.FeöfProc, 32,1875-1881,1973.
6 Jacquelyn J, Maher MD: Exploring alcohol's effects on liver function. Alcohol Health and Reasearch World, 21,5-12,1997.
7 Manno M, Bertazzon A, Burlina A, Galzigna L: Interaction of low doses of Ionizing radiation an carbon tetrachloride on liver superoxide dismutase and glutatione peroxidase in mice. Enzym, 34,107-112,1985.
8 Güven A, Erginsoy S, Kaya N: Kazlarda karbon tetraklorür toksikasyonunun lipid peroksidasyonuna bağlı olarak biyokimyasal ve patolojik parametrelere etkileri. Kafkas Üniv Vet Fak Derg, 9,131-136,2003.
9 Roig R, Cascan E, Arola L, Blade C, Salvado M: Effects of chronic wine and alcohol intake on glutathione and malondialdehyde levels in rats. Nutr Res, 20,1547-1550,2000.
10 Kanbak G, inal M, Baycu C: Ethanol-induced hepatoxicity and protective effect of betaine. Cell Biochem Funct, 19, 281-285, 2001.
11 Mizui T, Docteuchi M: Lipid peroxidaton: A possible role in gastric damage induced by ethanol in rats. Life Sci, 38, 2163-67, 1986.
12 Reacknagel RO: A new direction in the study of carbon tetrachloride hepatotoxicity. Life Sci, 33,401-408,1986.
13 French SW, Nash J, Shitabata P, Kachi K, Hara C, Chedid A, Mendenhall CL: Pathology of alcoholic liver disease. AV cooperative study group. Seminars in Liver Disease, 13, 154-169, 1993.
14 Freeman BM, Vince MA: Development of the avian embryo. Chopman and Hall, London. 4,459-463,1974.
15 Tappel AL: Measurement of and protection from in vivo lipid peroxidation, Free Radicals in Biology, 4, 1-47,1980.
16 Armbrust T, Batusic, Ringe B, Ramadar G: Mascells distribution in human liver diseose and experimental ratlive fibrosis, indications for mast cell participation in development of liver fibrosis.//&/?ato/, 26,1042-1054,1997.
17 Shreve DS, Levy HR: On the molecular weight of human glucose-6-phosphate dehydrogenase. Biochemical and Biophysical Research Communications, 78,1369-1375,1977.
18 Deutsch J: Glucose-6-phosphate dehydrogenase. In Bergmeyer HU, Bergmeyer J (Eds): Methods of Enzymatic Analysis, 3, 190-196,1983.
19 Ağar NS, Sandrzadeh S M, Hallaway PE, Eaton JW: Erythrocyte catalase. A somatic oxidant defence. J Clin Invest, 11, 319-321,1986.
20 Sies H: Antioxidant activity in cells and organs. A Rev Respir Dis, 136,478-480,1987.
21 Harris ED: Regulation of antioxsidant enzymes. FASEB J, 6, 2675-2683,1992.
22 Halliwell B, Gutteridge JM: Free radicals, lipid peroxidation and cell damage. Lancet, 2(8411), 1095,1984.
23 NRC: Nutrient repuirements of geese, in nutrient requirments of poultry. 9 th. Revised Edition National Academic Press. Washington D.C. 41,1994.
24 Placer ZA, Cushman LL, Johson BG: Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal Biochem, 16,359-364,1966.
25 Beutler E: Red cell metabolism: A Manual of Biochemical Methods. Grune and Station. New York. London 67-69,1975.
26 Aebi H: Catalase, In, Bergmeyer HU(Ed): Methods of Enzymmatic Analysis, H.U. Verlag Chenie: Deerfield Beach. FLYCH. 273-286,1987.
27 Lowry OH, Rosenbrough NJ, Farr AL, Randal RJ: Protein measurement with the folinuphenol reagent J Biol Chem, 193-265,1951.
28 International Federation of Clinical Chemistry (IFFC): Eur J Clin Chem Biochem, 25, 337-342, 1987.
29 Rikans LF, Hornbrook KR, Cai Y: Carbon tetrachloride hepatotoxicity as a function of age in female 344-rats. Mech Ageging Dev, 20,89-99,1994.
30 Lee NM, Bocker CE: Alcohol. In, Katzumg BG(Ed): Basic and Clinical Pharmacology, 278-289. Appleton and Lange, USA, 1989.
31 Bondy SC, Guo SX: Effect of ethanol treatment on indices of cumulation oxidative stress. European Journal Pharmacology, 270,349-355,1994.
32 Hernandez-Munoz R. Montical-Ruiz C, Vazopen-Martiny O: Gastric mucosal cell proliferation in ethanol-induced alergic mucusal injury is related to oxidative stress and lipid peroxidation in rats. Laboratory Investigation, 80,1161-1169,2000.
33 Vajdovich P, Szilagyi A, Gall T: Evcaluating of blood lipid peroxidation parameters in carbon tetrachloride(CCl) toxicity in sheep. Ada Veterinaria Hungarica, 43,4,87,1995.
34 Aleynik I.S, Leo AM, Ma Y, Aleynik KM, Lieber SC: Polyenylphosphatidylcholine prevents carbon tetrachloride-induced lipid peroxidation while it attenuates liver fibrosis. J Hepatol, 27,554-561,1997.
35 Shimuzzu M, Morita S, Yamano-Yamada A: Relationship between hipatic glutathione cantent and carbon tetrachloride- induced hepatotxicity in vivo. Toxicology Letters, 47, 95-102,1989.
36 Güven A, Güven A, Gülmez M: The effect of kefir on the activities of GSH-Px, GST, CAT, GSH and LPO levels in carbon tetrachloride-induced mice tissues. J Vet Med B 50, 412-416,2003.
37 Schaeffer F, Stainer RY: Glucose-6-phosphate dehidrogenase, kineties and molecular properties. Arc Microbiol, 116-119,1978.