Sevda ELİŞ YILDIZ, BUKET BAKIR, HASAN ASKER, Ebru KARADAĞ SARI

The Eff ects of the Basil (Ocimum sanctum) Treatment on the Tumor Necrosis Factor-α and Interleukin 1β Release in the Kidney Tissue of the Diabetic Rats

This study aims to examine the changes of the Ocimum sanctum treatment on the tumor necrosis factor alpha (TNF-α) and interleukin 1β (IL-1β)in the kidney tissue of the rats, in which the experimental diabetes was induced with streptozotocin (STZ). Forty Sprague Dawley male rats weredivided into 5 groups: Diabetes, Diabetes + Ocimum sanctum, Ocimum sanctum, Control, and Sham. The immunohistochemical localizationof TNF-α and IL-1β in the kidney tissue was determined by using the streptavidin-biotin-peroxidase method. Strong TNF-α immunoreactivitywas determined in the renal cortex of the rats in the Diabetes and Diabetes + Ocimum sanctum groups on 14th days, low immunoreactivitywas determined in the rats in Ocimum sanctum, Sham, and Control groups. While strong IL-1β immunoreactivity was observed in the renalcortex of the Diabetes group, moderate IL-1β immunoreactivity was observed in the renal cortex of the Diabetes + Ocimum sanctum and lowimmunoreactivity was determined in the Ocimum sanctum, Sham, and Control groups. In this study, it was assessed how the polymorphisms,occurring in the cytokine genes of Ocimum sanctum in the rats, in which experimental diabetes was induced, and TNF-α and IL-1β, which wasdemonstrated to have an important role in the complication development in the diabetic patients aff ected the renal tissue.

Diyabetik Ratlarda Fesleğen (Ocimum sanctum) Uygulamasının Böbrek Dokusunda Tümör Nekrozis Faktör-α ve İnterlökin 1β Salınımı Üzerine Etkileri

Bu çalışmada, fesleğen (Ocimum sanctum) uygulamasının, streptozotocin (STZ) ile deneysel diyabet oluşturulan ratların böbrek dokusunda tümör nekrozis faktör alfa (TNF-α) ve interlökin 1β (IL-1β) üzerine meydana getirdiği değişikleri incelemek amaçlanmıştır. Çalışmamızda toplam 40 adet Spraque Dawley cinsi erkek rat kullanıldı. Ratlar, Diyabet, Diyabet + Ocimum sanctum, Ocimum sanctum, Kontrol ve Sham olmak üzere 5 gruba ayrıldı. TNF-α ve IL-1β’in böbrek dokusundaki immunohistokimyasal lokalizasyonu streptavidin-biotin peroxidaz yöntemi ile belirlendi. Diyabet ve Diyabet + Ocimum sanctum uygulanmış grubun dışındaki grupların böbrek korteksinde zayıf düzeyde TNF-α immunoreaktivitesi tespit edildi. Diyabet grubunun böbrek korteksinde güçlü, Diyabet + Ocimum sanctum grubunda ise böbrek korteksinde orta düzeyde IL-1β immunoreaktivitesi tespit edilmesine rağmen Ocimum sanctum, Sham ve Kontrol grubundaki ratlarda ise zayıf düzeyde immunoreaktivite tespit edildi. Bu çalışma ile deneysel olarak diyabet oluşturulmuş ratlarda Ocimum sanctum’un sitokin genlerinde oluşan polimorfizmlerin diyabetli hastalarda komplikasyon gelişiminde önemli rol oynadıkları gösterilen TNF-α ve IL-1β’in böbrek dokusu üzerine ne kadar etkili olduğu değerlendirildi

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