Reza KHALKHALI EVRIGH, Kobra POURASAD, Reza Seyed SHARIFI, Vahid VAHEDI, Nemat HEDAYAT EVRIGH

Mitokondrial DNA Kullanılarak İran’daki Eski Dünya Develerinin (Camelus dromedaries ve Camelus Bactrianus) Genetik Farklılık ve Popülasyon Yapısının Araştırılması

Bu çalışma mitokondrial DNA kullanılarak İran develerinin genetik farklılığını araştırmak amacıyla yapılmıştır. Dört deve popülasyonundan toplam 120 devede 1052 bp cyt-b segmenti amplifiye edilmiş ve sekans analizi yapılmıştır. Elde edilen sonuçlar, çift hörgüçlü develerde 30 mutasyonun tek hörgüçlü develerde ise 9 mutasyonun olduğunu ve sırasıyla dokuz ve altı mtDNA haplotipinin bulunduğunu göstermiştir. Nükleotid farklılığı, tek ve çift hörgüçlü develer için sırasıyla 0.0015 ve 0.0121 olarak hesaplandı. Çift hörgüçlü deve popülasyonunda yüksek haplotip çeşitliliği (0.886±0.145) gözlemlenirken tek hörgüçlü deve popülasyonunda düşük genetik farklılaşma tespit edildi. Filogenetik analiz develer için farklı topluluklar gösterdi.

Genetic Diversity and Population Structure of Old World Camelids (Camelus dromedaries and Camelus Bactrianus) in Iran Using Mitochondrial DNA

This study was conducted to investigate the genetic diversity of Iranian camels using mitochondrial DNA. A fragment of 1052 bp cyt-b wasamplified and sequenced in 120 individuals from four camel populations. The results revealed that there are 30 mutations for Bactrian camelsand 9 mutations for dromedaries that lead to nine and six mtDNA haplotypes, respectively. Nucleotide diversity was calculated as 0.0015and 0.0121 for dromedaries and Bactrian camel populations respectively. We found high haplotype diversity in Bactrian camel populations(0.886±0.145) and low genetic differentiation among dromedary populations. Phylogeny analysis showed different cluster for camelids.

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