Buket BAKIR, Ebru KARADAĞ SARI, Sevda ELİŞ YILDIZ, Şükran YEDİEL ARAS, Serpil DAĞ

Tarçın Ekstraktı İle Tedavi Edilen Diyabetik Sıçanların Mide Dokusunda Somatostatinin İmmunohistokimyasal Dağılımı

Diyabet, kronik metabolik bir bozukluk olduğu gibi aynı zamanda da artmış bir oksidatif stres durumudur. Çalışmamızda immunohistokimyasal teknik kullanarak tarçın uygulanan streptozotosin ile diabet oluşturulan ratların mide dokusundaki somatostatinin salınımını inceledik. Çalışmada 30 adet Sprague Dawley cinsi erkek rat kullanıldı. Deney grupları kontrol, sham, tarçın, diyabet ve diyabet + tarçın olarak 5 gruba ayrıldı. Kontrol grubuna herhangi bir uygulama yapılmadı, sham grubuna intraperitoneal (i.p.) olarak 50 mg/kg sodyum sitrate uygulandı. Diyabet ve diyabet + tarçın gruplarına i.p. 50 mg/kg STZ enjeksiyonu yapılarak diabet oluşturuldu. Tarçın ve diyabet + tarçın gruplarına tarçın ekstraktı 200 mg/kg olacak şekilde oral gavaj yolu ile 14 gün verildi. Somatostatinin immunoreaktivitesini belirlemek için streptavidin-biotin-peroxidase metotu uygulandı. Mide dokularının normal histolojik yapısını incelemek için Crossman’ın üçlü boyama yöntemi ve Hematoksilen-Eosin boyaması uygulandı. Kontrol, sham ve tarçın gruplarındaki somotostatin immunoreaktivitesi, diyabet ve diyabet + tarçın gruplarından daha güçlü olduğu tesbit edildi. Diyabet ve diyabet + tarçın gruplarında mide dokusunun kardia, fundus ve pilor mukozasında zayıf immunoreaktivite bulunurken tarçın, sham ve kontrol gruplarında güçlü immunoreaktivite bulundu. Ayrıca tüm gruplar parietal ve prensipal hücre sayıları bakımından karşılaştırıldığında istatiksel olarak anlamlı bulundu (P

Immunohistochemical Distribution of Somatostatin in Gastric Tissue of Diabetic Rats Treated with Cinnamon Extract

Diabetes is a chronic metabolic disorder, as well as a situation of increased oxidative stress. We examined the distribution of somatostatin in gastrictissues of cinnamon extract treated streptozotocin-induced diabetic rats using the immunohistochemistry technique. A total of 30 male SpragueDawley rats were used in the study. The rats were assigned to five groups as control, sham, cinnamon, diabetes and diabetes + cinnamon. Noapplication was made to the control group, the sham group received intraperitoneally (i.p.) 50 mg/kg sodium citrate, and diabetes was inducedby i.p. injection of 50 mg/kg STZ in diabetes and diabetes + cinnamon groups. Cinnamon extracts were then given to cinnamon and diabetes+ cinnamon groups by oral gavage at a dose of 200 mg/kg for 14 days. The streptavidin-biotin-peroxidase method was used to determine theimmunoreactivity of somatostatin. Gastric tissue sections were prepared and stained by Crossman’s triple and Hematoxylin-Eosin staining in orderto examine histological structure of the gastric tissue. We determined that somatostatin immunoreactivity of the control, sham and cinnamongroups was stronger than for the diabetes, and diabetes + cinnamon groups. While a weak immunoreactivity was found in the cardia, fundusand pyloric mucosa of the gastric tissue in the diabetes and diabetes + cinnamon groups, a strong immunoreactivity was found in the cinnamon,sham, and control groups. Also, a statistically significant was observed when all groups compared in terms of count of parietal and principal cells(P

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1. Robertson RP, Harmon JS: Diabetes, glucose toxicity, and oxidative stress: A case of double jeopardy for the pancreatic islet beta cell. Free Radic Biol Med, 41 (2): 177-184, 2006. DOI: 10.1016/j.freeradbiomed. 2005.04.030
2. Hlebowicz J, Darwiche G, Björgell O, Almér LO: Effct of cinnamon on postprandial blood glucose, gastric emptying, and satiety in healthy subjects. Am J Clin Nutr, 85 (6): 1552-1556, 2007. DOI: 10.1093/ ajcn/85.6.1552
3. Kardiatun T, Dibua UM, Badger-Emeka L, Ugonabo JA, Tirwomwe JF, Agwu E, Ssamula M: The effct of cinnamon on glucose control in patients with type 2 diabetes mellitus in Pontianak, Indonesia. Int J Med Med Sci, 5 (10): 434-437, 2013.
4. Alanazi AS, Khan MU: Cinnamon use ın type 2 diabetes: An updated meta-analysıs. WJPPS, 4 (5): 1838-1852, 2015.
5. Ulbricht C, Seamon E, Windsor RC, Armbruester N, Bryan JK, Costa D, Grimes Serrano, JM, Tanguay-Colucci S, Weissner W, Yoon H, Zhang J: An evidence-based systematic review of cinnamon (Cinnamomum spp.) by the Natural Standard Research Collaboration. J Diet Suppl, 8 (4): 378- 454, 2011. DOI: 10.3109/19390211.2011.627783
6. Jia Q, Liu X, Wu X, Wang R, Hu X, Li Y, Huang C: Hypoglycemic activity of a polyphenolic oligomer-rich extract of cinnamomumparthenoxylon bark in normal and streptozotocin induced diabetic rats. Pythomedicine, 16, 744-750, 2009. DOI: 10.1016/j.phymed.2008.12.012
7. Kumar SS, Mukkadan JK: Anti diabetic effct of oral administration of cinnamon in wistar albino rats. BMJ, 2 (3): 97-99, 2013.
8. Broadhurst CL, Polansky MM, Anderson RA: Insulin-like biological activity of culinary and medicinal plant aqueous extracts in vitro. J Agric Food Chem, 48, 849-852, 2000. DOI: 10.1021/jf9904517
9. Anderson RA, Broadhurst CL, Polansky MM, Schmidt WF, Kahan A, Flanagan VP, Schoene NW, Graves DJ: Isolation and characterization of polyphenol type-a polymers from cinnamon with ınsulin-like biological activity. J Agric Food Chem, 52, 65-70, 2004. DOI: 10.1021/jf034916b
10. Şimşek ÜG, Çiftçi M, Doğan G, Özçelik M: Antioxidant activity of cinnamon bark oil (Cinnamomum zeylanicum L.) in japanese quails under thermo neutral and heat stressed conditions. Kafkas Univ Vet Fak Derg, 19 (5): 889-894, 2013. DOI: 10.9775/kvfd.2013.9049
11. Narin S, Piskin İE, Üstündag G: 2014’te Somatostatin’in tıp’ta kullanımı (Oktreotid). Güncel Gastroenterol, 18 (2): 272-276, 2014.
12.Demir can C, Kapıcıoğlu S, Kuşkonmaz İ, Taşkın A, Günaydın M, Kaya N: Mekanik intestinal obstruksiyonlu ratlarda somatostatin analogu SMS 201- 995 (Octreotide) ve omeprazolun etkileri. Uludağ Üniv Tıp Fak Derg, 29 (1): 11-14, 2003.
13. Timurkaan S, Timurkaan N, Ozkan E, Girgin M: Immunohistochemical distribution of somatostatin, glucagon and gastrin in the gastric fundus of the citellus (Spermophilus xanthoprymnus). J Anim Vet Adv, 8 (11): 2210-2214, 2009.
14. Gajdosik A, Gajdosikova A, Stefek M, Navarova J, Hozova R: Streptozotocin-induced experimental diabetes in male wistar rats. Gen Physiol Biophys, 18, 54-62, 1999.
15. Kanitkar M, Bhonde R: Existence of islet regenerating factors within in pancreas. Rev Diabet Stud, 1 (4): 185-192, 2004. DOI: 10.1900/ RDS.2004.1.185
16. Luna LG: Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 3 rd ed., 72-100, Mc Graw-Hill Book Comp, 1968.
17. Tru LD: Principles of immunohistochemistry. In, Tru LD (Ed): Atlas of Diagnostic Immunohistopathology. 1-31, New York Press. New York. 1990.
18. Shu S, Ju G, Fan L: The glucose oxidase-dab-nickel in peroxidase histochemistry of the nervous system. Neurosci Lett, 85, 169-171, 1988.
19. Zhu QY: Analysis of blood vessel invasion by cells of thyroid follicular carcinoma using image processing combined with immunohistochemistry. Zhonghua Yi Xue Za Zhi, 69, 573-575, 1989.
20. Seidal T, Balaton AJ, Battifora H: Interpretation and quantification of immunostains. Am J Surg Pathol, 25, 1204-1207, 2001.
21. Sun FP, Song Y G, Cheng W, Zhao T, Yao YL: Gastrin, somatostatin, G and D cells of gastric ulcer in rats. World J Gastroenterol, 8 (2): 375-378, 2002.
22. Bakır B, Karadağ Sarı E, Eliş Yıldız S, Asker H: Effcts of thymoquinone supplementation on somatostatin secretion in pancreas tissue of rats. Kafkas Univ Vet Fak Derg, 23 (3): 409-413, 2017. DOI: 10.9775/ kvfd.2016.16893
23. Cheng D, M Kuhn P, Poulev A, Rojo LE, Lila MA, Raskin I: In vivo and in vitro antidiabetic effcts of aqueous cinnamon extract and cinnamon polyphenol-enhanced food matrix. Food Chem, 135 (4): 2994- 3002, 2012. DOI: 10.1016/j.foodchem.2012.06.117
24. Kim SH, Hyun SH, Choung SY: Anti-diabetic effect of cinnamon extract on blood glucose in db/db mice. J Ethnopharmacol, 104 (1): 119- 123, 2006. DOI: 10.1016/j.jep.2005.08.059
25. Shokri G, Fathi H, Sabet MJ, Nasrabadi NN, Ataee R: Evaluation of anti-diabetic effcts of hydroalcoholic extract of green tea and cinnamon on streptozotocin-induced diabetic rats. Pharm Biomed Res, 1 (2): 20-29, 2015. DOI: 10.18869/acadpub.pbr.1.2.20
26. Kumar S, Vasudeva N, Sharma S: GC-MS analysis and screening of antidiabetic, antioxidant and hypolipidemic potential of cinnamomum tamala oil in streptozotocin induced diabetes mellitus in rats. Cardiovasc Diabetol, 11:95, 2012. DOI: 10.1186/1475-2840-11-95
27. Ranasinghe P, Perera S, Gunatilake M, Abeywardene E, Gunapala N, Premakumara S, Perera K, Lokuhetty D, Katulanda P: Effcts of cinnamon zeylanicum on blood glucose and lipids in a diabetic and healthy rat model. Pharmacognosy Res, 4 (2): 73-79. 97, 2012. DOI: 10.4103/0974-8490.94719
28. Rahman EANS, Abdel-Haleem AMH, Al Mudhaffar HM: Anti diabetic effcts of cinnamon powder and cinnamon aqueous extract on serum glucose of rats. IJFSNPH, 3 (2): 183-197, 2010.
29. O’Reilly D, Long RG: Diabetes and the gastrointestinal tract. Dig Dis, 5, 57-64, 1987. DOI: 10.1159/000171163
30. Takehara K, Tashima K, Takeuchi K: Alterations in duodenal bicarbonate secretion and mucosal susceptibility to acid in diabetic rats. Gastroenterology, 112, 418-428, 1997. DOI: 10.1053/gast.1997.v112. pm9024295
31. Weber JR, Ryan JC: Effcts on the gut of systemic disease and other extraintestinal conditions. In, Scharschmidt BF, Slei-singer MH, Feldman M (Ed): Gastrointestinal and liver disease. 6th ed., 413-416, WB Saunders Co, Philadelphia, 1998.
32. Bastaki SMA, Adeghate E, Chandranath IS, Amir N, Tariq S, Hameed RS, Adem A: Effcts of streptozotocin-induced long-term diabetes on parietal cell function and morphology in rats. Mol Cell Biochem, 341 (1-2): 43-50, 2010. DOI: 10.1007/s11010-010-0435-4
33. Subash-Babu P, Alshatwi AA, Ignacimuthu S: Beneficial antioxidative and antiperoxidative effect of cinnamaldehyde protect streptozotocin-induced pancreatic β-cells damage in wistar rats. Biomol Ther, 22 (1): 47-54, 2014. DOI: 10.4062/biomolther.2013.100
34. Kasacka I, Majewski M: An immunohıstochemıcal study of endocrine cells in the stomach of hypertensive rats. J Physiol Pharmacol, 58 (3): 469- 478, 2007.
35. Kasacka I, Łebkowski W, Janiuk I, Łapińska J, Lewandowska A: Immunohistochemical identification and localisation of gastrin and somatostatin in endocrine cells of human pyloric gastric mucosa. Folia Morphol, 71 (1): 39-44, 2012.
36. Chen M, He M, Peng K, Liu T, Jin C, Cao W, Wang L, Xiao K: An immunohistochemical study of somatostatin in the stomach and the small intestine of the African ostrich (Struthio camelus). Tissue Cell, 45 (6): 363-366, 2013. DOI: 10.1016/j.tice.2013.06.002