Zheng WANG, Renyue WEI, Jiaren ZHANG, Hongri RUAN, Tingtig ZHU, Jun SONG, Jiasan ZHENG

Köpeklerde Mekanik Hasar ve Alkali Yakma İle Oluşturulan Derin Korneal Ülserde Amniyon Sıvısı Süpernatantı Göz Damlası İle Birlikte Kullanılan Konjuktiva Örtüsünün Etkinliği

Bu çalışmanın amacı, alkali yakma ile birlikte mekanik hasar oluşturularak Beagle köpeklerde derin korneal ülser meydana getirmek suretiyle bir model oluşturmak ve oluşturulan bu derin korneal ülsere amniyon sıvısı süpernatantı ile birlikte korneal örtmenin etkisini araştırmaktır. Alkali yakma ve mekanik hasar ile derin korneal ülser oluşturmak amacıyla Beagle köpekler (n=15) rastgele olarak 3 gruba ayrıldı: Derin korneal ülser grubu (C grubu), konjuktival örtü grubu (T1 grubu) ve amniyon sıvısı süpernatantı göz damlası ile birlikte konjuktival örtü grubu (T2 grubu). Çalışmanın -3, 1, 7, 14, 21 ve 45. günlerinde, köpeklerin akuöz humorlarında İnterlökin-1 (IL-1), İnterlökin-8 (IL-8), Vasküler Endotelyal Büyüme Faktörü (VEGF), Matriks metalloproteinaz-1 (MMP-1), Matriks metalloproteinaz-2 (MMP-2) ve Matriks metalloproteinaz-9 (MMP-9) seviyeleri incelendi. Çalışmanın 45. gününde, her bir gruptan rastgele iki köpek seçilerek histopatolojik inceleme amacıyla korneal dokuları elde edildi. T1 ve T2 grubu ile karşılaştırıldığında C grubu köpek akuöz humorlarında IL-1, IL-8, VEGF, MMP-1, MMP-2, MMP-9 seviyelerinin anlamlı derecede azaldığı gözlemlendi (P

The Efficacy of Conjunctiva Coverage in Combination with Amnion Liquid Supernatant Eye Drop on Deep Layer Corneal Ulcer in Canine Caused by Alkali Burn Combined with Mechanical Injury

The purpose of this study was to establish the model of canine deep layer corneal ulcer by means of alkali burn combined with mechanical injury and evaluate the efficy of conjunctival covering combined with amnion liquid supernatant deep layer corneal ulcer in beagles. By using alkali burnand mechanical injury establishd the beagle deep layer corneal ulcer model (n=15) , randomly divided into 3 groups: deep layer corneal ulcer (C group), conjunctival covering group (T1 group), conjunctival covering combined amnion liquid supernatant eyedrop group (T2 group). On -3, 1, 7, 14, 21, 45 days, examine the levels of Interleukin -1 (IL-1), Interleukin-8 (IL-8), Vascular Endothelial Growth Factor (VEGF), Matrix metalloproteinase-1 (MMP-1), Matrix metalloproteinase-2 (MMP-2) and Matrix metalloproteinase-9 (MMP-9) in canine aqueous humor; On the 45 th day, two experimental canines were randomly selected from each group to collect corneal tissue for histopathological observation. C group compared to groups T1 group and T2 group , the levels of IL-1, IL-8, VEGF, MMP-1, MMP-2, MMP-9 in canine aqueous humor significantly decreased (P

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