Leyla MİS, Serdal KURT, Funda EŞKİ, Pınar AYVAZOĞLU DEMİR

Staphylococcus aureus İle Doğal Olarak Enfekte Olan Klinik veSubklinik Mastitisli İneklerin Süt ve Serumlarında Oksidatif Stres,Bağışıklık Sistemi ve Mineral Konsantrasyonlarının Değerlendirilmesi

Bu çalışmanın amacı, Staphylococcus aureus’un mastitisli ineklerin süt ve kan serumlarında oksidatif stres durumu (TAS, TOS, OSI), bağışıklık sistemi (IL1β, IL-6, TNF-α) ve mineral (Mg, Fe, Zn, Cu, Na ve Ca) konsantrasyonları üzerine etkisini araştırmaktı. Çalışma sağlıklı (Grup 1), klinik (Grup 2) ve subklinik mastitisli (Grup 3) inekler olmak üzere üç grup üzerinde yürütüldü. Grup 1 ve Grup 3’e göre Grup 2’de kan ve sütteki IL-1β düzeyleri arttı (P

Evaluation of Oxidative Stress, Immune System and Mineral Concentrations in Milk and Serum of Cows with Clinical and Subclinical Mastitis Naturally Infected by Staphylococcus aureus

The aim of this study was to investigate effect of Staphylococcus aureus on oxidative stress status (TAS, TOS, OSI), immune system (IL-1β, IL-6, TNF-α), and mineral (Mg, Fe, Zn, Cu, Na and Ca) concentrations in milk and serum of cows with mastitis. The cows were allocated to three groups according to mammary health status as follows: healthy (Group 1), clinical (Group 2) and subclinical mastitis cows (Group 3). IL-1β levels in serum and milk increased in Group 2 compared to Group 1 and Group 3 (P

PDF

___

1. Ma W, Wang Y, Gao F, Ning M, Liu A, Li Y, Gao Y, Lu P, Chen D: Development of a monoclonal antibody against bovine α-casein to evaluate functional status of mammary epithelial cells during mastitis. Kafkas Univ Vet Fak Derg, 25, 445-450, 2019. DOI: 10.9775/kvfd.2018.20897
2. Bradley AJ: Bovine mastitis: An evolving disease. Vet J, 164, 116-128, 2002. DOI: 10.1053/tvjl.2002.0724
3. Rusenova N, Gebreyes W, Koleva M, Mitev J, Penev T, Vasilev N, Miteva T: Comparison of three methods for routine detection of Staphylococcus aureus isolated from bovine mastitis. Kafkas Univ Vet Fak Derg, 19, 709-712, 2013. DOI: 10.9775/kvfd.2013.8753
4. Kurt S, Çolakoğlu HE, Yazlık MO, Vural MR, Küplülü, Ş: Sütçü ineklerde mastitis yönünden kuru ve geçiş dönemlerinin önemi. Atatürk Üniversitesi Vet Bil Derg, 14, 107-113, 2019. DOI: 10.17094/ataunivbd.407022
5. Saidi R, Cantekin Z, Khelef D, Ergün Y, Solmaz H, Kaidi R: Antibiotic susceptibility and molecular identification of antibiotic resistance genes of staphylococci isolated from bovine mastitis in Algeria. Kafkas Univ Vet Fak Derg, 21, 513-520, 2015. DOI: 10.9775/kvfd.2014.12836
6. Peralta OA, Carrasco C, Vieytes C, Tamayo MJ, Muñoz I, Sepulveda S, Tadich T, Duchens M, Melendez P, Mella A, Torres CG: Safety and efficacy of a mesenchymal stem cell intramammary therapy in dairy cows with experimentally induced Staphylococcus aureus clinical mastitis. Sci Rep, 10:2843, 2020. DOI: 10.1038/s41598-020-59724-7
7. Pereira UP, Oliveira DGS, Mesquita LR, Costa GM, Pereira LJ: Efficacy of Staphylococcus aureus vaccines for bovine mastitis: A systematic review. Vet Microbiol, 148, 117-124, 2011. DOI: 10.1016/j.vetmic.2010.10.003
8. Niedziela DA, Murphy MP, Grant J, Keane OM, Leonard FC: Clinical presentation and immune characteristics in first-lactation HolsteinFriesian cows following intramammary infection with genotypically distinct Staphylococcus aureus strains. J Dairy Res, 103, 8453-8466, 2020. DOI: 10.3168/jds.2019-17433
9. Zhang L, Hou X, Sun L, He T, Wei R, Pang M, Wang R: Staphylococcus aureus bacteriophage suppresses LPS-induced inflammation in MAC-T bovine mammary epithelial cells. Front Microbiol, 9:1614, 2018. DOI: 10.3389/fmicb.2018.01614
10. Alluwaimi AM, Leutenegger CM, Farver TB, Rossitto PV, Smith WL, Cullor JS: The cytokine markers in Staphylococcus aureus mastitis of bovine mammary gland. J Vet Med B Infect Dis Vet Public Health, 50, 105- 111, 2003. DOI: 10.1046/j.1439-0450.2003.00628.x
11. Alluwaimi AM: The cytokines of bovine mammary gland: Prospects for diagnosis and therapy. Res Vet Sci, 77, 211-222, 2004. DOI: 10.1016/j. rvsc.2004.04.006
12. Osman KM, Hassan HM, Ibrahim IM, Mikhail MMS: The impact of staphylococcal mastitis on the level of milk IL-6, lysozyme and nitric oxide. Comp Immunol Microbiol Infect Dis, 33, 85-93, 2010. DOI: 10.1016/j. cimid.2008.08.009
13. Ibrahim HMM, El-Seedy YY, Gomaa NA: Cytokine response and oxidative stress status in dairy cows with acute clinical mastitis. J Dairy Vet Anim Res, 3 (1): 00064, 2016. DOI: 10.15406/jdvar.2016.03.00064
14. Turk R, Piras C, Kovačić M, Samardžija M, Ahmed H, De Canio M, Urbani A, Meštrić ZF, Soggiu A, Bonizzi L, Roncada P: Proteomics of inflammatory and oxidative stress response in cows with subclinical and clinical mastitis. J Proteomics, 75, 4412-4428, 2012. DOI: 10.1016/j. jprot.2012.05.021
15. Abuelo A, Hernández J, Benedito JL, Castillo C: The importance of the oxidative status of dairy cattle in the periparturient period: Revisiting antioxidant supplementation. J Anim Physiol Anim Nutr, 99, 1003-1016, 2015. DOI: 10.1111/jpn.12273
16. Cenesiz S: The role of oxidant and antioxidant parameters in the infectious diseases: A systematic literature review. Kafkas Univ Vet Fak Derg, 26, 849-858, 2020. DOI: 10.9775/kvfd.2020.24618
17. Kleczkowski M, Kluciński W, Czerski M, Kudyba E: Association between acute phase response, oxidative status and mastitis in cows. Vet Stanica, 48, 177-186, 2017.
18. Sadek K, Saleh E, Ayoub M: Selective, reliable blood and milk biomarkers for diagnosing clinical and subclinical bovine mastitis. Trop Anim Health Prod, 49, 431-437, 2017. DOI: 10.1007/s11250-016-1190-7
19. Zhao X, Lacasse P: Mammary tissue damage during bovine mastitis: Causes and control. J Anim Sci, 86, 57-65, 2008. DOI: 10.2527/jas.2007-0302
20. Singh M, Yadav P, Sharma A, Garg VK, Mittal D: Estimation of mineral and trace element profile in bubaline milk affected with subclinical mastitis. Biol Trace Elem Res, 176, 305-310, 2017. DOI: 10.1007/s12011-016- 0842-9
21. Abd Ellah MR: Role of free radicals and antioxidants in mastitis. J Adv Vet Res, 3, 1-7, 2013.
22. Spears JW, Weiss WP: Role of antioxidants and trace elements in health and immunity of transition dairy cows. Vet J, 176, 70-76, 2008. DOI: 10.1016/j.tvjl.2007.12.015
23. Qayyum A, Khan JA, Hussain R, Avais M, Ahmad N, Khan MS: Investigation of milk and blood serum biochemical profile as an indicator of sub-clinical mastitis in Cholistani cattle. Pak Vet J, 36, 275-279, 2016.
24. Kuhla B: Review: Pro-inflammatory cytokines and hypothalamic inflammation: implications for insufficient feed intake of transition dairy cows. Animal, 14, 65-77, 2020. DOI: 10.1017/S1751731119003124
25. Amiri P, Rad AHF, Heidarpour M, Azizzadeh M, Khoramian B: Diagnostic accuracy of milk oxidation markers for detection of subclinical mastitis in early lactation dairy cows. Comp Clin Pathol, 29, 95-101, 2020. DOI: 10.1007/s00580-019-03024-8
26. Williamson JH, Lacy-Hulbert SJ: Effect of disinfecting teats post- milking or pre-and post-milking on intramammary infection and somatic cell count. N Z Vet J, 61, 262-268, 2013. DOI: 10.1080/00480169.2012.751576
27. Baştan A, Kaçar C, Acar DB, Şahin M, Cengiz M: Investigation of the incidence and diagnosis of subclinical mastitis in early lactation period cows. Turk J Vet Anim Sci, 32, 119-121, 2008.
28. Kandeel SA, Morin DE, Calloway CD, Constable PD: Association of California mastitis test scores with intramammary infection status in lactating dairy cows admitted to a veterinary teaching hospital. J Vet Intern Med, 32, 497-505, 2018. DOI: 10.1111/jvim.14876
29. Hogan JS, Gonzalez RN, Harmon RJ, Nickerson SC, Oliver SP, Pankey JW, Smith KL: Laboratory handbook on bovine mastitis. National Mastitis Council, Madison, Wisconsin, 1999.
30. Erel O: A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem, 37, 277-285, 2004. DOI: 10.1016/j.clinbiochem. 2003.11.015
31. Erel O: A new automated colorimetric method for measuring total oxidant status. Clin Biochem, 38, 1103-1111, 2005. DOI: 10.1016/j. clinbiochem.2005.08.008
32. Eşki F, Kurt S, Demir-Ayvazoğlu P: Effect of different estrus synchronization protocols on estrus and pregnancy rates, oxidative stress and some biochemical parameters in hair goats. Small Ruminant Res, 198:106348, 2021. DOI: 10.1016/j.smallrumres.2021.106348
33. Pedernera M, Celi P, García SC, Salvin HE, Barchia I, Fulkerson WJ: Effect of diet, energy balance and milk production on oxidative stress in early-lactating dairy cows grazing pasture. Vet J, 186, 352-357, 2010. DOI: 10.1016/j.tvjl.2009.09.003
34. Trevisi E, Jahan N, Bertoni G, Ferrari A, Minuti A: Pro-inflammatory cytokine profile in dairy cows: Consequences for new lactation. Ital J Anim Sci, 14 (3): 3862, 2015. DOI: 10.4081/ijas.2015.3862
35. Colakoglu HE, Kuplulu O, Vural MR, Kuplulu S, Yazlik MO, Polat IM, Oz B, Kaya U, Bayramoglu R: Evaluation of the relationship between milk glutathione peroxidase activity, milk composition and various parameters of subclinical mastitis under seasonal variations. Vet Arhiv, 87, 557-570, 2017. DOI: 10.24099/vet.arhiv.160728
36. Bannerman DD: Pathogen-dependent induction of cytokines and other soluble inflammatory mediators during intramammary infection of dairy cows. J Anim Sci, 87, 10-25, 2009. DOI: 10.2527/jas.2008-1187
37. Yang C, Liu P, Wang S, Zhao G, Zhang T, Guo S, Jiang K, Wu H, Deng G: Shikonin exerts anti-inflammatory effects in LPS-induced mastitis by inhibiting NF-κB signaling pathway. Biochem Biophys Res Commun, 505, 1-6, 2018. DOI: 10.1016/j.bbrc.2018.08.198
38. Akhtar M, Guo S, Guo YF, Zahoor A, Shaukat A, Chen Y, Umar T, Deng PG, Guo M: Upregulated-gene expression of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) via TLRs following NF-κB and MAPKs in bovine mastitis. Acta Trop, 207:105458, 2020. DOI: 10.1016/j.actatropica. 2020.105458
39. Guo YF, Xu NN, Sun W, Zhao Y, Li CY, Guo MY: Luteolin reduces inflammation in Staphylococcus aureus-induced mastitis by inhibiting NFκB activation and MMPs expression. Oncotarget, 8, 28481-28493, 2017. DOI: 10.18632/oncotarget.16092
40. Yang W, Zerbe H, Petzl W, Brunner RM, Günther J, Draing C, von Aulock S, Schuberth HJ, Seyfert HM: Bovine TLR2 and TLR4 properly transduce signals from Staphylococcus aureus and E. coli, but S. aureus fails to both activate NF-κB in mammary epithelial cells and to quickly induce TNFα and interleukin-8 (CXCL8) expression in the udder. Mol Immunol, 45, 1385-1397, 2008. DOI: 10.1016/j.molimm.2007.09.004
41. Sakemi Y, Tamura Y, Hagiwara K: Interleukin-6 in quarter milk as a further prediction marker for bovine subclinical mastitis. J Dairy Res, 78, 118-121, 2011. DOI: 10.1017/S0022029910000828
42. Nakajima Y, Mikami O, Yoshioka M, Motoi Y, Ito T, Ishikawa Y, Yasukawa K: Elevated levels of tumor necrosis factor-α, (TNF-α) and interleukin-6 (IL-6) activities in the sera and milk of cows with naturally occurring coliform mastitis. Res Vet Sci, 62, 297-298, 1997. DOI: 10.1016/ S0034-5288(97)90209-5
43. Hagiwara K, Yamanaka H, Hisaeda K, Taharaguchi S, Kirisawa R, Iwai H: Concentrations of IL-6 in serum and whey from healthy and mastitic cows. Vet Res Commun, 25, 99-108, 2001. DOI: 10.1023/a:1006400801305
44. Bochniarz M, Zdzisińska B, Wawron W, Szczubiał M, Dąbrowski R: Milk and serum IL-4, IL-6, IL-10, and amyloid A concentrations in cows with subclinical mastitis caused by coagulase-negative staphylococci. J Dairy Sci, 100, 9674-9680, 2017. DOI: 10.3168/jds.2017-13552
45. Shaheen T, Ahmad SB, Rehman MU, Muzamil S, Bhat RR, Hussain I, Bashir N, Mir MUR, Paray BA, Dawood MAO: Investigations on cytokines and proteins in lactating cows with and without naturally occurring mastitis. J King Saud Univ Sci, 32 (6): 2863-2867, 2020. DOI: 10.1016/j. jksus.2020.07.009
46. Ohtsuka H, Kudo K, Mori K, Nagai F, Hatsugaya A, Tajima M, Tamura K, Hoshi F, Koiwa M, Kawamura SI: Acute phase response in naturally occurring coliform mastitis. J Vet Med Sci, 63, 675-678, 2001. DOI: 10.1292/jvms.63.675
47. Fusco R, Cordaro M, Siracusa R, Peritore AF, D’Amico R, Licata P, Crupi R, Gugliandolo E: Effects of hydroxytyrosol against lipopolysaccharide-induced inflammation and oxidative stress in bovine mammary epithelial cells: A natural therapeutic tool for bovine mastitis. Antioxidants, 9(8):693, 2020. DOI: 10.3390/antiox9080693
48. Sordillo LM, Aitken SL: Impact of oxidative stress on the health and immune function of dairy cattle. Vet Immunol Immunopathol, 128, 104- 109, 2009. DOI: 10.1016/j.vetimm.2008.10.305
49. Yang FL, Li XS: Role of antioxidant vitamins and trace elements in mastitis in dairy cows. J Adv Vet Anim Res, 2, 1-9, 2015. DOI: 10.5455/ javar.2015.b48
50. Puppel K, Kalińska A, Kot M, Slósarz J, Kunowska-Slósarz M, Grodkowski G, Kuczyńska B, Solarczyk P, Przysucha T, Gołębiewski M: The effect of Staphylococcus spp., Streptococcus spp. and Enterobacteriaceae on the development of whey protein levels and oxidative stress markers in cows with diagnosed mastitis. Animals, 10 (9):1591, 2020. DOI: 10.3390/ ani10091591
51. Tabatabaee N, Heidarpour M, Khoramian B: Milk metabolites, proteins and oxidative stress markers in dairy cows suffering from Staphylococcus aureus subclinical mastitis with or without spontaneous cure. J Dairy Res, 88, 326-329, 2021. DOI: 10.1017/S0022029921000613
52. Chen SJ, Zhang CY, Yu D, Lin CJ, Xu HJ, Hu CM: Selenium alleviates inflammation in Staphylococcus aureus-induced mastitis via MerTKdependent activation of the PI3K/Akt/mTOR pathway in mice. Biol Trace Elem Res, 2021 (in press). DOI: 10.1007/s12011-021-02794-z
53. Andrei S, Matei S, Fit N, Cernea C, Ciupe S, Bogdan S, Groza IS: Glutathione peroxidase activity and its relationship with somatic cell count, number of colony forming units and protein content in subclinical mastitis cows milk. Rom Biotechnol Lett, 16, 6209-6217, 2011.
54. Mahapatra A, Panigrahi S, Patra RC, Rout M, Ganguly S: A study on bovine mastitis related oxidative stress along with therapeutic regimen. Int J Curr Microbiol App Sci, 7, 247-256, 2018. DOI: 10.20546/ ijcmas.2018.701.027
55. Dalanezi FM, Joaquim SF, Guimaraes FF, Guerra ST, Lopes BC, Schmidt EMS, Cerri RLA, Langoni H: Influence of pathogens causing clinical mastitis on reproductive variables of dairy cows. J Dairy Sci, 103, 3648-3655, 2020. DOI: 10.3168/jds.2019-16841
56. Singh R, Bhardwaj RK, Azad MS, Beigh SA: Effect of mastitis on haemato-biochemical and plasma mineral profile in crossbred cattle. Indian J Anim Res, 48, 63-66, 2014. DOI: 10.5958/j.0976-0555.48.1.013
57. Al-Autaish HHN: Clinical, hematological and serological study of subclinical mastitis in local cows in Basrah Province. QJVMS, 18, 99-104, 2019.
58. Gera S, Guha A, Sharma A, Manocha V: Evaluation of trace element profile as an indicator of bovine sub-clinical mastitis. Intas Polivet, 12, 9-11, 2011.
59. Enjalbert F, Lebreton P, Salat O: Effects of copper, zinc and selenium status on performance and health in commercial dairy and beef herds: Retrospective study. J Anim Physiol Anim Nutr (Berl), 90, 459-466, 2006. DOI: 10.1111/j.1439-0396.2006.00627.x
60. Kandeel SA, Megahed AA, Constable PD: Evaluation of hand‐ held sodium, potassium, calcium, and electrical conductivity meters for diagnosing subclinical mastitis and intramammary infection in dairy cattle. J Vet Intern Med, 33, 2343-2353, 2019. DOI: 10.1111/jvim.15550