Fatemeh BASTAMPOOR, Seyed Ebrahim HOSSEINI, Mehrdad SHARIATI, Mokhtar MOKHTARI

Kurşun Toksikasyonu Oluşturulan Ratlarda Maydanoz Yapraklarının(Petroselinum crispum) Sulu-Alkollü Ekstraktı Karaciğer HasarınıHafifletir

Kurşun (Pb) zehirlenmesi ve indüklenen lezyonun potansiyeli, başta karaciğer olmak üzere vücudun diğer birçok sistemi üzerine zararlı etkileri olan küresel bir endişedir. Bu çalışmada, antioksidan özelliğinde önemli bir rol oynayabilecek flavonoidlerin bir bileşenini içeren maydanoz yapraklarının (PAE) sulu-alkollü ekstraktının, kurşun tarafından indüklenen karaciğer hasarını önlemedeki etkinliğini araştırdık. Bunun için altmış yetişkin erkek rat, faktöriyel bir düzenle; kontrol grubu; 2 mL su ile 21 gün boyunca oral gavaj yapılan sham grubu; 500 ppm kurşun uygulanan grup; kurşun ile kombine halde 100 mg/kg ve 200 mg/kg PAE uygulanan gruplar ve 200 mg/kg PAE uygulanan grup olmak üzere rastgele altı gruba ayrıldı. Kan serumlarında karaciğer stresi için karaciğer enzimleri ve oksidatif stres indeksleri hesaplandı. Karaciğer gen ekspresyonunun değerlendirilmesinde apoptoz seviyeleri ölçüldü. Veriler, kurşun uygulamasının karaciğer ağırlığını ve yem alımını azalttığını gösterdi. Kontrol grubu ile karşılaştırıldığında kurşun uygulanan grubun kan serumundaki karaciğer enzimlerinin içeriğinin önemli ölçüde arttığı saptandı. Ayrıca, kurşun uygulanan gruba ait ratların MDA içerikleri, kontrol grubundan önemli ölçüde daha fazlaydı. Benzer şekilde, T-AOC ve CAT ve SOD aktiviteleri önemli ölçüde azalmıştı. Aynı zamanda, kurşun uygulaması, ratlarda Bax ve TNF-α gen ekspresyonlarını artırıp Bcl-2 gen ekspresyonunu azaltarak karaciğer apoptozisi ile ilgili genleri indükledi. Bununla beraber, PAE uygulaması, karaciğerin oksidatif ve apoptozis değişikliklerini önemli ölçüde iyileştirdi. Bu nedenle, PAE’nın antioksidan aktivitesiyle bu çalışma, kurşun kaynaklı toksisitede yeni bir mekanistik yaklaşım sağlamaktadır.

Exposure to Aqueous-Alcoholic Extract of Parsley Leaves (Petroselinum crispum) in Lead-Treated Rats Alleviate Liver Damage

Lead (Pb) poisoning and the induced potential of the lesion is a global concern with harmful effects on multiple body systems, particularly the liver system. In this study, we have investigated the efficacy of aqueous-alcoholic extract of parsley leaves (PAE), which is a component of flavonoids that could play an important role in the antioxidant property, in preventing liver Pb-damages. For this, sixty adult male rats were randomly divided into six groups in a factorial arrangement: control; receiving oral gavage for 21 days with 2 mL water; 500 ppm Pb; 100 and 200 mg/kg PAE in combination form with Pb, and 200 mg/kg PAE. Liver enzymes and oxidative stress indexes were computed for liver stress, in blood serum. Apoptosis levels were assessed in the evaluation of liver gene expression. Data indicated that Pb reduced liver weight and feed intake. The results showed that Pb significantly increased the liver enzymes contents in the blood serum in the comparison vehicle group. Furthermore, the MDA contents of the Pb group rats were significantly more than that of the vehicle group. Likewise, T-AOC and the activities of CAT and SOD were significantly reduced. Meanwhile, Pb administration induced liver apoptosis-related genes by upregulating Bax and TNF-α genes and downregulating the Bcl-2 gene of animals. At the same time, administration of PAE significantly improved liver oxidative and apoptosis changes. Thus, this study provides a novel mechanistic approach concerning Pb-induced toxicity, due to PAE antioxidant activity.

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