ELVAN ANADOL, ATİYE SEDA YAR SAĞLAM, Gul Fatma Yarim, NİLGÜN GÜLTİKEN

Serum Concentrations of Anti-Müllerian Hormone and its Expression in the Remnant Ovarian Tissue of Rats with Experimentally Induced Ovarian Remnant Syndrome

Anti-Müllerian hormon (AMH), testislerde Sertoli hücrelerinden ve ovaryumlarda granuloza hücrelerinden sentezlenmektedir. AntiMüllerian hormonun ovaryum kökenli olmasından dolayı, memelilerde ovaryum varlığının veya kalan ovaryum dokusunun tespit edilmesinde kullanılabilmektedir. Bu çalışmanın amacı, deneysel olarak ovaryum kalıntısı sendromu oluşturulan ratlarda serum AMH konsantrasyonları ve ovaryohisterektomi ile uzaklaştırılan ovaryum dokusunda ve kalan ovaryum dokusundaki AMH gen ekspresyonlarını karşılaştırmaktı. Çalışmada toplam 18 Sprague Dawley rat kullanıldı. Deneysel olarak ovaryum kalıntısı sendromu oluşturulan 6 rat grup I'i, her iki ovaryumu da uzaklaştırılan 6 rat grup II'yi ve sham operayonu yapılan 6 rat ise grup III'ü oluşturdu. Bütün gruplarda genel anestezi altında median laparotomi gerçekleştirildi. AMH mRNA ekspresyonu kantitatif gerçek zamanlı polimeraz zincir reaksiyonu (qRT-PCR) ile ölçüldü. Operasyon sonrası 30. günde, 0. güne kıyasla AMH mRNA ekspresyon düzeylerinde azalma oldu (P>0.05). Operasyondan 10 gün sonra ortalama AMH değeri grup I, II ve III'de sırasıyla 2.27±0.52 ng/ml,

Deneysel Olarak Ovaryum Kalıntısı Sendromu Oluşturulan Ratlarda Serum Anti-Müllerian Hormon Konsantrasyonu ve Kalıntı Ovaryum Dokusunda AMH mRNA Ekspresyonu

Anti-Müllerian hormone (AMH) is synthesised in the Sertoli cells of the testes and granulosa cells of the ovary. As the ovaries seem to be the primary source of AMH, it may be used for determination of the presence or absence of ovaries or ovarian remnants in mammalians. The purpose of the present study was to compare the serum AMH concentration of rats with experimentally induced ovarian remnant syndrome and the expression of AMH in the ovarian tissue removed during ovariohysterectomy and remnant ovarian tissue. A total of eighteen Sprague Dawley rats were used in the study. Group I consisted of 6 rats that were gone through ovarian remnant syndrome (ORS) experimentally, group II consisted of 6 rats in which both ovaries were removed and group III consisted of 6 rats that were sham-operated. Median laparotomy was performed in the all groups under general anaesthesia. AMH mRNA expression was determined using Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). AMH mRNA expression levels in group I were decreased on day 30 after surgery when compared to day 0 (P>0.05). Mean concentration of serum AMH on day 10 after surgery in group I, II and III were found 2.27±0.52 ng/ml, <0.312 ng/ml and 3.96±0.53 ng/ml, respectively (P<0.05). In conclusion, this finding suggests that evaluation of serum AMH concentration could be an useful method to determine the presence or absence of ovaries or ovarian remnants in the rat.

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