Lazar MAKIVIC, Milica GLISIC, Marija BOSKOVIC, Jasna DJORDJEVIC, Radmila MARKOVIC, Milan BALTIC, Dragan SEFER

Performances, Ileal and Cecal Microbial Populations and Histological Characteristics in Broilers Fed Diets Supplemented with Lignocellulose

The effect of dietary lignocellulose on broilers performance, intestinal microbiota and morphology, pH of digesta and litter humidity after 28 and 42 days of the experiment was evaluated. A total of 384 Cobb500 chickens (initial weight: 41.88±1.56 g) were divided into 4 groups with 24 replications and fed with control diet (C), a control diet with added 0.4% of lignocellulose (T1), a diet with added 0.6% of lignocellulose at the expense of soybean meal and maize (T2), and a diet supplemented with 0.6% of lignocellulose at the expense of soybean meal (T3). T2 treatment significantly influenced body weight, weight gain (WG), feed intake (FI) and feed conversion ratio (FCR). T2 and T3 treatment increased average LAB and Bifidobacterium spp. count, and decreased the number of Escherichia coli in the ileum and cecum, while differences in cecal Clostridium perfringens count among 0.4% and 0.6% treatments were not observed. Feeding the lignocellulose diet did not affect the relative weights of empty proventriculus, gizzard or intestines, but led to a decrease in pHs. T3 treatment caused an increase of the villi heights and significantly lower moisture content in the litter. Even though the addition of lignocellulose into broilers diet positively influenced performances, changes in intestinal microbiota and villi heights, based on the results of the present study, supplementation with 0.6% lignocellulose is recommended.

Lignoselüloz İlaveli Diyetle Beslenen Etlik Piliçlerde Performans, İleal ve Sekal Mikrobiyal Popülasyon İle Histolojik Özellikler

Diyetteki lignoselülozun 28 ve 42 gün uygulama sonrası broiler tavuklarda performans, barsak mikrobiyatı, morfolojisi ve içerik pH’sı ile dışkı nemi üzerine etkileri değerlendirildi. Toplam 384 adet Cobb500 tavuk (ilk ağırlıkları: 41.88±1.56 g) 24 tekrar olmak üzere 4 gruba ayrıldı ve kontrol diyet (C), %0.4 lignoselüloz ilaveli kontrol diyet (T1), soya fasulyesi ve mısır yerine %0.6 lignoselüloz ilaveli diyet (T2), soyafasulyesi yerine %0.6 lignoselüloz ilaveli diyet (T3) ile beslendi. T2 uygulaması vücut ağırlığı, ağırlık kazanımı, yem tüketimi ve yem konversiyon oranını anlamlı derecede etkiledi. T2 ve T3 uygulamaları ileum ve sekumda ortalama LAB ve Bifidobacterium spp. sayısını artırırken Escherichia coli sayısında azalmaya neden oldu. %0.4 ile %0.6 uygulamaları arasında sekum Clostridium perfringens miktarında ise fark belirlenmedi. Lignoselüloz ilaveli diyetle besleme proventrikulus, taşlık ve barsakların orantısal organ ağırıklarını etkilemezken pH’da azalmaya neden oldu. T3 uygulaması, villus uzunluklarında artmaya ve anlamlı derecede dışkının neminde azalmaya neden oldu. Broiler diyetine lignoselüloz ilavesi performans, barsak mikrobiyatası ve villus uzunluğunda pozitif etkilere neden olmuştur. Elde edilen sonuçlar doğrultusunda %0.6 oranında lignoselüloz kullanılması önerilmektedir.

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1. JøRgensen H, Zhao XQ, Knudsen KEB, Eggum BO: The influence of dietary fibre source and level on the development of the gastrointestinal tract, digestibility and energy metabolism in broiler chickens. Br J Nutr, 75 (3): 379-395, 1996. DOI: 10.1079/BJN19960141

2. Smits CH, Veldman A, Verkade HJ, Beynen AC: The inhibitory effect of carboxymethylcellulose with high viscosity on lipid absorption in broiler chickens coincides with reduced bile salt concentration and raised microbial numbers in the small intestine. Poult Sci, 77 (10): 1534-1539, 1998. DOI: 10.1093/ps/77.10.1534

3. Iji PA, Saki AA, Tivey DR: Intestinal development and body growth of broiler chicks on diets supplemented with non-starch polysaccharides. Anim Feed Sci Technol, 89 (3-4): 175-188, 2001. DOI: 10.1016/S0377- 8401(00)00223-6

4. Farran MT, Akilian HA, Hamoud AM, Barbour GW, Saoud IP: Lignocellulose improves protein and amino acid digestibility in roosters and egg hatchability in broiler breeders. J Poult Sci, 54 (3): 197-204, 2017. DOI: 10.2141/jpsa.0160095

5. Lamid M, Kusriningrum, Al-Arif A, Warsito SH: Improving the quality of rice bran by utilization of lignocellulosic enzymes for broiler feed. J Appl Environ Biol Sci, 5 (12): 227-230, 2015.

6. Svihus B, Hetland H: Ileal starch digestibility in growing broiler chickens fed on a wheat-based diet is improved by mash feeding, dilution with cellulose or whole wheat inclusion. Br Poult Sci, 42 (5): 633-637, 2001. DOI: 10.1080/00071660120088461

7. Krás RV, Kessler AM, Ribeiro AML, Henn J, Dos Santos II, Halfen DP, Bockor L: Effect of dietary fiber and genetic strain on the performance and energy balance of broiler chickens. Rev Bras Cienc Avic, 15 (1): 15-19, 2013. DOI: 10.1590/S1516-635X2013000100003

8. Bogusławska-Tryk M, Szymeczko R, Piotrowska A, Burlikowska K, Śliżewska K: Ileal and cecal microbial population and short-chain fatty acid profile in broiler chickens fed diets supplemented with lignocellulose. Pak Vet J, 35 (2): 212-216, 2015.

9. Farran MT, Pietsch M, Chabrillat T: Effect of lignocellulose on the litter quality and the ready to cook carcass yield of male broilers. Actes des 10èmes Journées de la Recherche Avicole et Palmipèdes à Foie Gras du 26 au 28 mars, 2013, La Rochelle, France, 917-921, 2013.

10. Vantress C: Cobb broiler management guide. Cobb-Vantress, Siloam Springs, AR, USA, 2012.

11. Association of Official Analytical Chemists. Official Method of Analysis. 16th ed., AOAC. Washington, DC, 1995.

12. Smirnov A, Perez R, Amit-Romach E, Sklan D, Uni Z: Mucin dynamics and microbial populations in the chicken small intestine are changed by dietary probiotic and antibiotic growth promoter supplements. J Nutr, 135 (2): 187-192, 2005. DOI: 10.1093/jn/135.2.187

13. Yamabayashi S: Periodic acid-Schiff-Alcian Blue: A method for the differential staining of glycoproteins. Histochem J, 19 (10-11): 565-571, 1987. DOI: 10.1007/BF01687364

14. Aptekmann KP, Artoni SMB, Stefanini MA, Orsi MA: Morphometric analysis of the intestine of domestic quails (Coturnix coturnix Japonica) treated with different levels of dietary calcium. Anat Histol Embryol, 30 (5): 277-280, 2001. DOI: 10.1046/j.1439-0264.2001.00331.x

15. ISO 1442:1997. Meat and meat products - Determination of moisture content (Reference method), 1997.

16. Bogusławska-Tryk M, Piotrowska A, Szymeczko R, Burlikowska K, Głowińska B: Lipid metabolism indices and fatty acids profile in the blood serum of broiler chickens fed a diet with lignocellulose. Rev Bras Cienc Avic, 18 (3): 451-456, 2016. DOI: 10.1590/1806-9061-2015-0157

17. Sarikhan M, Shahryar HA, Gholizadeh B, Hosseinzadeh MH, Beheshti B, Mahmoodnejad A: Effects of insoluble fiber on growth performance, carcass traits and ileum morphological parameters on broiler chick males. Int J Agric Biol, 12 (4): 531-536, 2010.

18. González-Alvarado JM, Jiménez-Moreno E, González-Sánchez D, Lázaro R, Mateos GG: Effect of inclusion of oat hulls and sugar beet pulp in the diet on productive performance and digestive traits of broilers from 1 to 42 days of age. Anim Feed Sci Technol, 162 (1-2): 37-46, 2010. DOI: 10.1016/j.anifeedsci.2010.08.010

19. Bogusławska‐Tryk M, Piotrowska A, Szymeczko R, Burlikowska K: Effect of dietary lignocellulose on ileal and total tract digestibility of fat and fatty acids in broiler chickens. J Anim Physiol Anim Nutr, 100 (6): 1050- 1057, 2016. DOI: 10.1111/jpn.12476

20. Jiménez-Moreno E, Chamorro S, Frikha M, Safaa HM, Lázaro R, Mateos GG: Effects of increasing levels of pea hulls in the diet on productive performance, development of the gastrointestinal tract, and nutrient retention of broilers from one to eighteen days of age. Anim Feed Sci Technol, 168 (1-2): 100-112, 2011. DOI: 10.1016/j.anifeedsci.2011.03.013

21. Mateos GG, Jiménez-Moreno E, Serrano MP, Lázaro RP: Poultry response to high levels of dietary fiber sources varying in physical and chemical characteristics. J Appl Poult Res, 21 (1): 156-174, 2012. DOI: 10.3382/japr.2011-00477

22. Yokhana JS, Parkinson G, Frankel TL: Effect of insoluble fiber supplementation applied at different ages on digestive organ weight and digestive enzymes of layer-strain poultry. Poult Sci, 95 (3): 550-559, 2015. DOI: 10.3382/ps/pev336

23. Guinotte F, Gautron J, Nys Y, Soumarmon A: Calcium solubilization and retention in the gastrointestinal tract in chicks (Gallus domesticus) as a function of gastric acid secretion inhibition and of calcium carbonate particle size. Br J Nutr, 73 (1): 125-139, 1995. DOI: 10.1079/BJN19950014

24. Jiménez-Moreno E, González-Alvarado JM, de Coca-Sinova A, Lázaro R, Mateos GG: Effects of source of fibre on the development and pH of the gastrointestinal tract of broilers. Anim Feed Sci Technol, 154 (1-2): 93-101, 2009. DOI: 10.1016/j.anifeedsci.2009.06.020

25. Sklan D, Smirnov A, Plavnik I: The effect of dietary fibre on the small intestines and apparent digestion in the turkey. Br Poult Sci, 44 (5): 735- 740, 2003. DOI: 10.1080/00071660310001643750

26. Kimiaeitalab MV, Goudarzi SM, Jiménez-Moreno E, Cámara L, Mateos GG: A comparative study on the effects of dietary sunflower hulls on growth performance and digestive tract traits of broilers and pullets fed a pullet diet from 0 to 21 days of age. Anim Feed Sci Technol, 236, 57- 67, 2018. DOI: 10.1016/j.anifeedsci.2017.11.023

27. Oke DB, Oke MO: Effects of feeding graded levels of sawdust obtained from Daniellia ogea tree on the performance and carcass characteristics of broiler chickens. Res J Poult Sci, 1 (1): 12-15, 2007.

28. Hetland H, Svihus B, Choct M: Role of insoluble fiber on gizzard activity in layers. J Appl Poult Res, 14 (1): 38-46, 2005. DOI: 10.1093/japr/14.1.38

29. Roberts T, Wilson J, Guthrie A, Cookson K, Vancraeynest D, Schaeffer J, Moody R, Clark S: New issues and science in broiler chicken intestinal health: Intestinal microbial composition, shifts, and impacts. Worlds Poult Sci J, 71 (2): 259-270, 2015. DOI: 10.1017/S0043933915000276

30. Abazari A, Navidshad B, Mirzaei Aghjehgheshlagh F, Nikbin S: The effect of rice husk as an insoluble dietary fiber source on intestinal morphology and Lactobacilli and Escherichia coli populations in broilers. Iran J Vet Med, 10 (3): 217-224, 2016. DOI: 10.22059/IJVM.2016.58684

31. Baurhoo B, Ruiz-Feria CA, Zhao X: Purified lignin: Nutritional and health impacts on farm animals-A review. Anim Feed Sci Technol, 144 (3-4): 175-184, 2008. DOI: 10.1016/j.anifeedsci.2007.10.016

32. O’Shea EF, Cotter PD, Stanton C, Ross RP, Hill C: Production of bioactive substances by intestinal bacteria as a basis for explaining probiotic mechanisms: bacteriocins and conjugated linoleic acid. Int J Food Microbiol, 152 (3): 189-205, 2012. DOI: 10.1016/j.ijfoodmicro.2011.05.025

33. Cao BH, Zhang XP, Guo YM, Karasawa Y, Kumao T: Effects of dietary cellulose levels on growth, nitrogen utilization, retention time of diets in digestive tract and cecal microflora of chickens. Asian-Australas J Anim Sci, 16 (6): 863-866, 2003. DOI: 10.5713/ajas.2003.863

34. Stappenbeck TS, Hooper LV, Gordon JI: Developmental regulation of intestinal angiogenesis by indigenous microbes via Paneth cells. Proc Natl Acad Sci U S A, 99 (24): 15451-15455, 2002. DOI: 10.1073/ pnas.202604299

35. Youssef IMI, Beineke A, Rohn K, Kamphues J: Experimental study on effects of litter material and its quality on foot pad dermatitis in growing turkeys. Int J Poult Sci, 9 (12): 1125-1135, 2010. DOI: 10.3923/ ijps.2010.1125.1135