Zafer CANTEKİN, YAŞAR ERGÜN, GÖKHAN DOĞRUER, MUSTAFA KEMAL SARIBAY, HASAN SOLMAZ

Comparison of PCR and culture methods for diagnosis of subclinical mastitis in dairy cattle

Sığır mastitisleri süt sığırcılığı endüstrisinde ekonomik kayıpların en önemli nedenidir. Mastitiste etkeninin hızlı ve kesin tanısı hastalığın tedavisi ve kontrolü açısından çok önemlidir. Bu çalışmanın amacı subklinik sığır mastitislerinde etkenin tanısı için kültür ve polimeraz zincir reaksiyonu tekniklerinin karşılaştırılmasıdır. Bu amaçla, 540 ineğin California Mastitis Test ile muayenesinden sonra, pozitif bulunan 79 adet süt örneği, klasik kültür metodu ve direkt sütten yapılan polimeraz zincir reaksiyonu ile analiz edildi. Kırk üç örnekte her iki yöntemde de mikroorganizma belirlenirken, sadece 7 örnekte iki teknik arasından fark görüldü. Bu 7 örnekte kültür yöntemiyle sadece Koagülaz Negatif Stafilok belirlenirken, polimeraz zincir reaksiyonu ile Koagülaz Negatif Stafilok dışında S. dysgalactiae yönünden de pozitif bulundu. Bu çalışmanın sonuçları polimeraz zincir reaksiyonunun kültür yönteminden daha duyarlı olduğunu ve direkt olarak süt numunesinden hedef etkenin bir kaç saat içinde saptanabileceğini gösterdi. Hızlı ve güvenilir moleküler tekniklerin kullanımı mastitiste sürüden çıkarma veya tedavi etme konusunda hızlı karar verilebilmesinde yardımcı olabilir.

Süt sığırlarında sub-klinik mastitisin tanısında kültür ve PCR yöntemlerinin karşılaştırılması

Bovine mastitis is the greatest source of economic loss in the dairy industry. Rapid and definitive detection of causative agent is very important for treatment and control of the disease. The aim of this study was to compare culture and polymerase chain reaction techniques for diagnosis of agents in subclinical bovine mastitis. For this purpose, after conducting the California Mastitis Test on 540 cows, 79 milk samples were analysed by the classical culture method and simplex polymerase chain reaction. Forty-three of samples were found positive by both methods, diferences were found only seven samples. While coagulase negative staphylococci these seven samples were determined by culture method, coagulase negative staphylococci and S. dysgalactiae were determined together by polymerase chain reaction. The results of this study indicate that the polymerase chain reaction is more sensitive than culture method and could detect pathogens at the species level within a few hours from directly milk samples. Rapid and relieable molecular techniques can be useful method in farm level detection for fast decision about the culling or treatment.

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